Comparison of Isotope Techniques for Determining True Absorption of Dietary Manganese in Growing Rats<sup>1</sup>

Weigand, E.; Kirchgeßner, M.; Helbig, Ursula

doi:10.1111/j.1439-0396.1986.tb00544.x

Cited by 9 publications

(1 citation statement)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Digestibility of organic matter, crude protein and NfE were observed to be lower while feeding a ration with 43% vinasse in DM in comparison with a ration of moderate vinasse content (16% in DM), but the difference was only significant for protein digestibility. Until now, only few investigations dealing with the digestibility of vinasse in pigs have been published (Boeve et al., 1973; Weigand and Kirchgessner, 1980a,b, 1981). Therefore, there are no German tabulated data.…”

Section: Resultsmentioning

confidence: 99%

Beet‐vinasse (condensed molasses solubles) as an ingredient in diets for cattle and pigs – nutritive value and limitations

Stemme

Gerdes

Harms

et al. 2005

Animal Physiology Nutrition

View full text Add to dashboard Cite

Sugar beet vinasse, originating as the condensed molasses residue from yeast production, was fed to bulls and pigs to determine the feed value. It contained, per kg dry matter (DM content 66.8%), 293 g crude protein, 305 g N-free extract (therefrom 34.4 g total sugar), and 395 g crude ash. The experiment was carried out with three fattening bulls (German Holstein) and four adult pigs (Gottingen Minipig). In bulls, two balance trials lasting 20 days each (10 days adaptation; 10 days collection) were conducted in which subsequently a basal ration without and with vinasse (14% in DM) was fed. In pigs, the experiment consisted of three 12-day balance trials with the final 5 days as collection period. In the first trial, pigs received a basal diet without vinasse and in the following, a mixed diet with 16 and 43% vinasse (in DM) respectively. Digestibilities of components in vinasse in bulls were: organic matter 73.5%, crude protein 72.6%, and N-free extract 52.3%. In bulls, no influence on faeces quality could be detected while feeding 14% vinasse in DM. While feeding 16% vinasse in DM to pigs, the digestibility of organic matter amounted to 72.3% (crude protein 71.8%; NfE 74.6%). Feeding higher amounts of vinasse (43% in DM) in the diet of pigs, digestibilities were reduced (organic matter: 61.6%), which might be a result of a reduced retention time of the chyme as a result of an osmotically driven diarrhoea because of unexpectedly high sulphate (136 g/kg DM) content in the vinasse. It is concluded that because of organic matter digestibility >70% for cattle and pigs, the use of vinasse in these species in small proportions is reasonable.

show abstract

Section: Resultsmentioning

confidence: 99%

Beet‐vinasse (condensed molasses solubles) as an ingredient in diets for cattle and pigs – nutritive value and limitations

Stemme

Gerdes

Harms

et al. 2005

Animal Physiology Nutrition

View full text Add to dashboard Cite

show abstract

Radioisotope-dilution technique for determining endogenous manganese in feces of the growing rat

Weigand

Helbig

Kirchgeßner

1986

Biol Trace Elem Res

View full text Add to dashboard Cite

A conventional balance study with growing rats was conducted to evaluate experimental conditions for determining endogenous fecal manganese (Mn) excretion and, hence, true Mn absorption by the isotope-dilution technique. Thirty-four rats, with a mean initial live weight of 60 g, allotted to three groups of 8 animals and one group of 10 animals, were injected intramuscularly with a(54)Mn tracer dose and sacrificed after 4, 8, 12, and 16 d, respectively.In liver and serum, the specific radioactivity of Mn was the lowest among the tissues analyzed and its exponential rate of decrease over the period of d 4-16 was the highest. During the 8-d period, from d 9-16, apparent Mn absorption averaged 14.1% of intake (128.5 μg Mn/d). Assuming that the specific activity of Mn in liver of d 11 or, alternatively, in serum of d 16, was on the average representative of that of endogenous Mn in feces of d 9-16, it was computed that Mn of endogenous origin accounted for 9.0 and 9.3% of the total fecal excretion of the metal, and that true absorption amounted to 21.9 and 22.1% of Mn intake, respectively.

show abstract

Endogenous excretion and true absorption of cobalt as affected by the oral supply of cobalt

Kirchgeßner

Reuber

Kreuzer

1994

Biol Trace Elem Res

View full text Add to dashboard Cite

At the end of a 49-d experiment with 32 growing male rats, a period of 8 d was used to determine endogenous excretion and true absorption as well as apparent absorption and retention of cobalt with the aid of the isotope dilution technique. For this purpose, a single im dose of 58Co was applied at d 35 of the experiment. After that, urine and feces were collected separately from d 8 to 15 after injection of the isotope. The specific cobalt activity of the liver was used as an endogenous reference source. The basal diet provided 5.9 ppb cobalt, the different treatment groups were obtained by supplementing the diet with 0, 10, 50, 250, or 1250 ppb cobalt. The different diets were offered from the beginning of the experiment. In the balance period, apparent and true absorption as well as fecal excretion behaved similar to cobalt intake, whereas urinary excretion increased more rapidly with increasing cobalt supply. Endogenous fecal excretion accounted for 3.5 ng Co/d in the groups fed the diets without and with 10 ppb cobalt. An increase was not observed until supplementing the diet with 50 ppb cobalt. This increase between 250 and 1250 ppb cobalt was higher than the corresponding increase in the dietary cobalt supply. This indicates that endogenous fecal excretion might be more important for homeostatic regulation at a higher dietary cobalt concentration. Endogenous renal excretion as calculated from the results of the isotope dilution technique showed a similar kind of response to increasing cobalt supply as endogenous fecal loss. Nevertheless, the elimination of excessive cobalt mainly took place by adjusting urinary excretion, whereas the variations in true absorption and endogenous fecal excretion had no quantitative importance. Apparent and true absorption were on average 28.0 and 29.8%, respectively, of the cobalt intake. In the case of retention, a marked decline was observed from 19% in the depletion group to 3% with 1250 ppb cobalt, again demonstrating the importance of urinary excretion for controlling the cobalt content of the organism.

show abstract

Comparison of Isotope Techniques for Determining True Absorption of Dietary Manganese in Growing Rats¹

Cited by 9 publications

References 7 publications

Beet‐vinasse (condensed molasses solubles) as an ingredient in diets for cattle and pigs – nutritive value and limitations

Beet‐vinasse (condensed molasses solubles) as an ingredient in diets for cattle and pigs – nutritive value and limitations

Radioisotope-dilution technique for determining endogenous manganese in feces of the growing rat

Endogenous excretion and true absorption of cobalt as affected by the oral supply of cobalt

Contact Info

Product

Resources

About

Comparison of Isotope Techniques for Determining True Absorption of Dietary Manganese in Growing Rats1

Cited by 9 publications

References 7 publications

Beet‐vinasse (condensed molasses solubles) as an ingredient in diets for cattle and pigs – nutritive value and limitations

Beet‐vinasse (condensed molasses solubles) as an ingredient in diets for cattle and pigs – nutritive value and limitations

Radioisotope-dilution technique for determining endogenous manganese in feces of the growing rat

Endogenous excretion and true absorption of cobalt as affected by the oral supply of cobalt

Contact Info

Product

Resources

About

Comparison of Isotope Techniques for Determining True Absorption of Dietary Manganese in Growing Rats¹