Comparison of laser ablation-inductively coupled plasma-mass spectrometry and micro-X-ray fluorescence spectrometry for elemental imaging in Daphnia magna

Gholap, Deepti Sahebrao; Izmer, Andrei; Samber, Björn De; Elteren, Johannes van; Šelih, Vid Simon; Evens, Roel; Schamphelaere, Karel A.C. De; Janssen, Colin R.; Balcaen, Lieve; Lindemann, Inge; Vincze, László; Vanhaecke, Frank

doi:10.1016/j.aca.2010.01.052

Cited by 65 publications

(42 citation statements)

References 19 publications

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“…Nevertheless, absolute levels of quantified metals varied between the methods in the range from 70.8 to 164%, with the highest variance seen in Fe levels, although within-brain regional differences in metal levels were accurately reflected in using both methods. Previous work using both XFM and LA-ICPMS imaging of the crustacean Daphnia magna showed that both techniques produce comparable distribution maps and demonstrated that both techniques exhibit advantages, depending on the specific analyte of interest, 43 though, in contrast to the data we present here, this study did not directly compare quantitative images from both methods. Direct comparison of quantitative approaches using both XFM and inductively coupled plasmaoptical emission spectrometry (ICP-OES) for the production of thin film reference materials for XFM showed that, while agreement was good, variation of up to 11% was still observed between the techniques for a relatively noncomplex sample matrix.…”

Section: Analytical Chemistrycontrasting

confidence: 68%

Comparative Study of Metal Quantification in Neurological Tissue Using Laser Ablation-Inductively Coupled Plasma-Mass Spectrometry Imaging and X-ray Fluorescence Microscopy

et al. 2015

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Redox-active metals in the brain mediate numerous biochemical processes and are also implicated in a number of neurodegenerative diseases. A number of different approaches are available for quantitatively measuring the spatial distribution of biometals at an image resolution approaching the subcellular level. Measured biometal levels obtained using laser ablation-inductively coupled plasma mass spectrometry (LA-ICPMS; spatial resolution 15 μm × 15 μm) were within the range of those obtained using X-ray fluorescence microscopy (XFM; spatial resolution 2 μm × 7 μm) and regional changes in metal concentration across discrete brain regions were replicated to the same degree. Both techniques are well suited to profiling changes in regional biometal distribution between healthy and diseased brain tissues, but absolute quantitation of metal levels varied significantly between methods, depending on the metal of interest. Where all possible variables affect metal levels, independent of a treatment/phenotype are controlled, either method is suitable for examining differences between experimental groups, though, as with any method for imaging post mortem brain tissue, care should be taken when interpreting the total metal levels with regard to physiological concentrations.

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Section: Analytical Chemistrycontrasting

confidence: 68%

Comparative Study of Metal Quantification in Neurological Tissue Using Laser Ablation-Inductively Coupled Plasma-Mass Spectrometry Imaging and X-ray Fluorescence Microscopy

et al. 2015

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“…An interesting comparison between the elemental imaging capabilities of LA-ICP-MS and micro X-ray fluorescence (XRF) spectrometry was carried out for the model organism Daphnia magna, in terms of detection power and spatial resolution, by Gholap et al [29]. Experimental results showed that the spatial resolution of LA-ICP-MS seems to be higher than that provided by micro-XRF.…”

Section: Optimisation Of the Laser And Icp-ms Parametersmentioning

confidence: 97%

Laser ablation ICP-MS for quantitative biomedical applications

et al. 2012

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LA-ICP-MS allows precise, relatively fast, and spatially resolved measurements of elements and isotope ratios at trace and ultratrace concentration levels with minimal sample preparation. Over the past few years this technique has undergone rapid development, and it has been increasingly applied in many different fields, including biological and medical research. The analysis of essential, toxic, and therapeutic metals, metalloids, and nonmetals in biomedical tissues is a key task in the life sciences today, and LA-ICP-MS has proven to be an excellent complement to the organic MS techniques that are much more commonly employed in the biomedical field. In order to provide an appraisal of the fast progress that is occurring in this field, this review critically describes new developments for LA-ICP-MS as well as the most important applications of LA-ICP-MS, with particular emphasis placed on the quantitative imaging of elements in biological tissues, the analysis of heteroatom-tagged proteins after their separation and purification by gel electrophoresis, and the analysis of proteins that do not naturally have ICP-MS-detectable elements in their structures, thus necessitating the use of labelling strategies.

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“…Although laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) is still not widely used in plant analysis, it provides a possibility to analyze solid samples and permits sensitive visualization of elemental distribution within the plant (126)(127)(128)). An additional advantage, especially in ionomic and genetic research, is the ability to investigate thin sections of biological tissues both quantitatively and visually.…”

Section: Instrumental Measurementmentioning

confidence: 99%

Multielement Analytical Spectroscopy in Plant Ionomics Research

Djingova

Mihaylova

Lyubomirova

et al. 2013

Applied Spectroscopy Reviews

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The study of the ionome (ionomics) is defined as quantitative and simultaneous measurement of the element composition of living organisms and changes in this composition in response to physiological stimuli, development stage, and genetic modifications (Salt et al., Ann. Rev. Plant Biol., Vol. 59, 2008). The necessity of understanding the regulation processes of elements in the organisms demands determination of many elements in the organism, tissue, and cell (Baxter, Plant Biol., Vol. 12, 2009) . A prospect for ionomics is environmental pollution where great variety of conditions and pollutants exist resulting in concentration and interelemental changes in the plant ionome. The capabilities of and problems with several multielement analytical techniques, including instrumental neutron activation analysis (INAA), X-ray fluorescence, inductively coupled plasma-atomic emission spectrometry (ICP-AES), inductively coupled plasma-mass spectrometry (ICP-MS), and atomic absorption spectrometry (AAS), which are adequate and most promising in ionomic and environmental studies of plants, are reviewed. References are confined mainly to the last 10-15 years. Information about concentrations, roles, binding forms, and pollution sources of the elements and comparison between methods with respect to limits of detection, determined elements, interferences, and economic considerations are tabulated. Some combinations of instrumental techniques supplementing each other are highly valued, namely, ICP-MS and ICP-AES and INAA and AAS or ICP-AES.

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Comparison of laser ablation-inductively coupled plasma-mass spectrometry and micro-X-ray fluorescence spectrometry for elemental imaging in Daphnia magna

Cited by 65 publications

References 19 publications

Comparative Study of Metal Quantification in Neurological Tissue Using Laser Ablation-Inductively Coupled Plasma-Mass Spectrometry Imaging and X-ray Fluorescence Microscopy

Comparative Study of Metal Quantification in Neurological Tissue Using Laser Ablation-Inductively Coupled Plasma-Mass Spectrometry Imaging and X-ray Fluorescence Microscopy

Laser ablation ICP-MS for quantitative biomedical applications

Multielement Analytical Spectroscopy in Plant Ionomics Research

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