Comparison of LC and UPLC Coupled to MS–MS for the Determination of Sulfonamides in Egg and Honey

Tamošiūnas, Vytautas; Padarauskas, Audrius

doi:10.1365/s10337-008-0579-5

Cited by 22 publications

(8 citation statements)

References 25 publications

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“…Some authors compared the performance of HPLC-MS/MS and UHPLC-MS/MS, obtaining narrower peaks and better chromatographic resolution using UHPLC-MS/MS, with separation of 10 sulfonamides in less than 20 min [48], although shorter running times can be achieved (<10 min) [8].…”

Section: Veterinary Drugsmentioning

confidence: 99%

Comprehensive analysis of toxics (pesticides, veterinary drugs and mycotoxins) in food by UHPLC-MS

Frenich¹,

Aguilera-Luiz²

2014

TrAC Trends in Analytical Chemistry

117

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Section: Veterinary Drugsmentioning

confidence: 99%

Comprehensive analysis of toxics (pesticides, veterinary drugs and mycotoxins) in food by UHPLC-MS

Frenich¹,

Aguilera-Luiz²

2014

TrAC Trends in Analytical Chemistry

117

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“…The sample matrix was defined as the leading factor. As secondary factors which might influence the analysis, the operator, the SPE cartridge, the storage period of the extracts, and the extract evaporation temperature were chosen [27]. Four concentration levels for each analyte were used in the validation study (Table 3).…”

Section: Analytical Performancementioning

confidence: 99%

Ultra performance liquid chromatography-tandem mass spectrometry for the determination of 5-nitroimidazoles and their metabolites in egg

Tamošiūnas

Padarauskas

2009

Open Chemistry

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An ultra performance liquid chromatography (UPLC) coupled to tandem mass spectrometry (MS/MS) procedure was developedfor the determination of five 5-nitroimidazoles (dimetridazole, ipronidazole, metronidazole, ronidazole and ternidazole) and three of their metabolites (1-methyl-2-hydroxymethyl-5-nitroimidazole, 1-(2-hydroxyethyl)-2-hydroxymethyl-5-nitroimidazole and 1-methyl-2-(2'-hydroxyisopropyl)-5-nitroimidazole) in egg matrices. Conditions for UPLC separation and electrospray ionization MS/MS in the positive ion mode were optimized. Samples were prepared by liquid-liquid extraction with buffered aqueous 2.5% trichloroacetic acid followed by solid-phase extraction on a Strata-X-C cartridge with reversed-phase and cation-exchange functionalities. The method's performance was evaluated in accordance with Commission Decision 2002/657/EC, applying the alternative matrix-comprehensive in-house validation approach using specially designed InterVal software. The method was robust against different sample matrix and SPE cartridges, operator change, and changes in sample extract storage. Acceptable apparent recoveries (76 to 109%) were obtained for all analytes. The decision limits (CC α ) and detection capabilities (CC β ) were within the ranges of 0.19 -2.62 and 0.26 -4.29 μg kg -1. For all compounds the calibration curve linearity was good with correlation coefficients greater than 0.99. Fifteen eggs and ten whole egg powder samples obtained commercially in Lithuania were analyzed by UPLC-MS/MS; none were found contaminated by 5-nitroimidazoles or their metabolites. Warsaw and Springer-Verlag Berlin Heidelberg. © Versita

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“…The liquid-liquid extraction (LLE) (Anderson et al, 2005) and solid-phase extraction (SPE) (Pailler et al, 2009) have been extensively applied to the pretreatment of liquid food samples. However, most of these methods are time consuming and usually toxic due to the use of organic solvents (Yang et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, analysis by liquid chromatography followed by fluorescence or ultra-violet detection systems can give low detection limits (Maudens et al, 2004;Pang et al, 2003;Posyniak et al, 2003;Sheridan et al, 2008) Recently, the basic advances in developing sensitivity and specifity of food analyses of pharmaceutical residues are due to the novel technology instrument, the application of ultra-performance liquid chromatography with tandem mass spectrometric detection (UPLC-MS/MS), which is more sophisticated technique allows a very effective isolation of analyte ions from the noise-producing matrix. The UPLC-MS/MS produces a high speed of analysis, greater resolution, higher peak capacity and sensitivity (Swartz 2005;Tamošiūnas & Padarauskas, 2008).…”

Section: Introductionmentioning

confidence: 99%

Development of a rapid method for the determination of antibiotic residues in honey using UPLC-ESI-MS/MS

Kıvrak

Harmandar

2016

Food Sci. Technol

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An accurate, reliable and fast multianalyte/multiclass ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the simultaneous analysis of 23 pharmaceuticals, belonging to different classes amphenicols, sulfonamides, tetracyclines, in honey samples. The method developed consists of ultrasonic extraction followed by UPLC-ESI-MS/MS with electrospray ionization in both positive mode and negative mode. The influence of the extraction solvents and mobile phase composition on the sensitivity of the method, and the optimum conditions for sample weight and extraction temperature in terms of analyte recovery were extensively studied. The identification of antibiotics is fulfilled by simultaneous use of chromatographic separation using an Acquity BEH C 18 (100 mm x 2.1 mm, 1.7 µm) analytical column with a gradient elution of mobile phases and tandem mass spectrometry with an electrospray ionization. Finally, the method developed was applied to the determination of target analytes in honey samples obtained from the local markets and several beekeepers in Muğla, Turkey. Ultrasonic-extraction of pharmaceuticals from honey samples is a well-established technique by UPLC-ESI-MS/MS, the uniqueness of this study lies in the simultaneous determination of a remarkable number of compounds belonging to 23 drug at the sub-nanogram per kilogram level.Keywords: antibiotics; fast method; honey; UPLC-ESI-MS/MS; chloramphenicol.Practical Application: Honey is one of the healthy foods across the world. Food safety is an important issue regarding residues of pharmaceutical drugs. The developed method proposed simultaneous analysis of analysis of twenty-three veterinary pharmaceuticals in honey. A fast and sensitive multianalyte/multiclass ultra-performance liquid chromatography-tandem mass spectrometry with electrospray ionization (UPLC-ESI-MS/MS) method provides the analysis of 50 honey samples in 50 minutes for food and laboratory sectors, and benefits safe food consumption.

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Comparison of LC and UPLC Coupled to MS–MS for the Determination of Sulfonamides in Egg and Honey

Cited by 22 publications

References 25 publications

Comprehensive analysis of toxics (pesticides, veterinary drugs and mycotoxins) in food by UHPLC-MS

Comprehensive analysis of toxics (pesticides, veterinary drugs and mycotoxins) in food by UHPLC-MS

Ultra performance liquid chromatography-tandem mass spectrometry for the determination of 5-nitroimidazoles and their metabolites in egg

Development of a rapid method for the determination of antibiotic residues in honey using UPLC-ESI-MS/MS

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