Comparison of Liquid Chromatographic and Bioassay Procedures for Determining Depletion of Intramuscularly Injected Tylosin

Moats, W. A.; Harris, Elmer W.; Steele, N. C.

doi:10.1093/jaoac/68.3.413

Cited by 4 publications

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“…Preliminary assays showed that the recovery of tylosin from pig liver and kidney progressively decreased when the spiked homogenates were maintained at room temperature pending extraction. As suggested by Moats et al 3 adequate recoveries from liver and kidney samples could be obtained only by lowering the pH of the homogenate before tylosin addition. A tentative explanation is the occurrence, in liver and kidney homogenates, of residual enzyme activity (inhibited by acidification) responsible for the degradation of tylosin.…”

Section: Discussionmentioning

confidence: 95%

“…EU legislation has established 1 a maximum residue limit (MRL) of 100 mg kg 21 for porcine edible tissues. Currently available methods [2][3][4][5] for the detection of tylosin at the MRL level are either laborious or require significant volumes of solvents (often chlorinated solvents), and are generally suitable for only one or two tissues. Hence for control purposes a reliable and simple method for the determination of tylosin in edible pig tissues is needed.…”

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confidence: 99%

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Determination of tylosin residues in pig tissues using high-performance liquid chromatography

Liguoro

Montesissa²,

Anfossi³

et al. 1998

Analyst

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In accordance with the maximum residue limit of 100 micrograms kg-1 established by EU legislation, a simple and sensitive high-performance liquid chromatographic (HPLC) method was developed for the measurement of tylosin residues in pig tissues (fat, kidney, liver and muscle). Tylosin, a macrolide antibiotic, is extracted with water-methanol and cleaned-up by solid-phase extraction (SPE) on cation-exchange cartridges using methanol elution. Tylosin was determined by reversed-phase HPLC with UV detection at 280 nm and the mean recovery from pig tissues fortified in the range 50-200 micrograms kg-1 was 70-85%, with intra- and inter-day RSDs in the ranges 3.4-9.1 and 3.9-10.1% respectively.

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Section: Discussionmentioning

confidence: 95%

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confidence: 99%