Comparison of malondialdehyde levels and superoxide dismutase activity in resveratrol and resveratrol/donepezil combination treatment groups in Alzheimer’s disease induced rat model

Rao, Y. Lakshmisha; Ganaraja, B; Marathe, Aradhana; Manjrekar, Poornima; Joy, Teresa; Ullal, Sonali; Pai, Mángala M.; Nayak, Vanishri S.

doi:10.1007/s13205-021-02879-5

Cited by 35 publications

(25 citation statements)

References 41 publications

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“…After the last dose and fasting for 12 hours, take blood from the mouse abdominal aorta, centrifuge, and take serum and plasma; then, take pancreatic tissue, homogenize, and determine glycosylated hemoglobin (HbAlc) [ 12 ], serum resistin (RESISTEIN) [ 13 ], and serum insulin (FINS) [ 14 ] according to the instructions of the enzyme linked immunosorbent assay (ELISA) kit, and calculate the islet sensitivity index (ISI) and insulin resistance index (HOMA-IR) according to the following formulas: ISI = ln[1/(FBG × FINS)]; HOMA-IR = FBG × FINS/22.5 [ 15 ]. Then, determine superoxide dismutase (SOD) [ 16 ], malondialdehyde (MDA) [ 17 ], and nitric oxide (NO) [ 18 ] in pancreatic tissue content.…”

Section: Experimental Methodsmentioning

confidence: 99%

Study on the Effects of Chinese Materia Medica Processing on the Hypoglycemic Activity and Chemical Composition of Anemarrhenae Rhizoma

Wang

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Purpose. To compare the hypoglycemic effects of different extracts of Anemarrhenae Rhizoma (AR) before and after being stir-baked with salt water on the diabetic mice and to detect the contents of 8 components in the corresponding active parts simultaneously using the UPLC-MS method, in order to screen the better extracts for diabetes and to clear the material basis for enhancing hypoglycemic activity of Anemarrhenae Rhizoma stir-baked with salt water (SAR). Methods. Taking spontaneous type II diabetic db/db mice as models and fasting blood glucose (FBG), oral glucose tolerance test (OGTT), glycated hemoglobin or glycosylated hemoglobin (HbAlc), serum resistin (RESISTEIN), fasting insulin (FINS), superoxide dismutase (SOD), malondialdehyde (MDA), and nitric oxide (NO) as indicators, the hypoglycemic effects of different active parts of Anemarrhenae Rhizoma were evaluated. The chromatographic separation was performed on a Waters BEH C18 (2.1 mm × 50 mm, 1.7 μm) column using acetonitrile (B) and 0.1% formic acid in water (A) as mobile phases, and the flow rate was 0.3 ml/min. The column temperature was set as 28°C, and the injection volume was 10 μL. A mass spectrometer was connected to the UPLC system via an electrospray ionization (ESI) interface. Full-scan data acquisition was performed in the negative ion mode. Result. In the study of pharmacodynamics, the hypoglycemic effect of Anemarrhenae Rhizoma stir-baked with salt water is better than that of Anemarrhenae Rhizoma and the hypoglycemic effect of ethanol extract of Anemarrhenae Rhizoma is more remarkable than that of the decoction. The measured components all have a good linear relationship within their respective linear ranges (r ≥ 0.9990); the average recovery rates are 98.86%–100.69%, RSD <2.90%. Compared with the raw Anemarrhenae Rhizoma, the contents of Timosaponin AIII, Timosaponin BII, Timosaponin BIII, Anemarrhenasaponin I, Anemarrhenasaponin Ia, and Mangiferin of Anemarrhenae Rhizoma stir-baked with salt water are all higher, the changes of Timosaponin AI and Anemarrhenasaponin AII are not obvious, and all the contents of chemical composition in the ethanol extract of Anemarrhenae Rhizoma and Anemarrhenae Rhizoma stir-baked with salt water were obviously higher compared with the water decoction. Conclusion. The processing method, stir-baking with salt water, can increase the contents of active compositions in Anemarrhenae Rhizoma and strengthen the hypoglycemic effect. The ethanol extract of Anemarrhenae Rhizoma stir-baked with salt water is the better active site.

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Section: Experimental Methodsmentioning

confidence: 99%

Study on the Effects of Chinese Materia Medica Processing on the Hypoglycemic Activity and Chemical Composition of Anemarrhenae Rhizoma

Wang

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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“…Given this, and since the swimming speed was the same among the groups, it could be assumed that the observed differences in memory function may not be related to confounding factors such as speci c differences in sensorimotor integration or motivation of the animals. Donepezil in combination with a variety of agents such as piperine (Nazi et al, 2021), resveratrol (Rao et al, 2021), estradiol (Gibbs et al, 2011), and manual acupuncture (Jiang et al, 2019) has been examined to achieve a superior bene t for neurobehavioral de cits in AD animal models. To date, there have been no attempts to address the potentials of donepezil administration and EE in combination for the prevention of cognitive de cits in AD patients or experimentally neurodegenerative status.…”

Section: Discussionmentioning

confidence: 99%

“…The combination of different interventions for achieving superior cognitive enhancement has appeared as a promising therapeutic approach in health and disease. In the case of cognitive de cits in AD and other experimental models, the combination of donepezil with some treatments including piperine (Nazi et al, 2021), resveratrol (Rao et al, 2021), estradiol (Gibbs et al, 2011), and manual acupuncture (Jiang et al, 2019) has been explored. However, to date, no study has addressed the combination of donepezil and EE for cognitive impairments in experimentally neurodegenerative status.…”

Section: Ee Increases the Expression Of Neurotrophic Factors And Othe...mentioning

confidence: 99%

Combination Therapy of Donepezil and Environmental Enrichment on Memory Deficits in Amyloid-Beta-Induced Alzheimer's Disease Rats

Gholami

Negah

Rajabian

et al. 2021

Preprint

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Alzheimer's disease (AD) is progressive neurodegeneration known as the most common cause of dementia, and it is the sixth leading cause of death in older people. Given the promising data on the additive effect of combination therapy with donepezil (Aricept), an acetylcholinesterase inhibitor (AChEI), and regarding the similar neuronal mechanisms through them donepezil and environmental enrichment (EE) exert their enhancing effects on cognition; we asked whether simultaneous treatment with two paradigms in amyloid-beta-induced AD rats may lead to greater cognitive improvements than either treatment individually. AD was induced by intrahippocampal injection of amyloid-beta (1-42, 6 µg), and donepezil was orally administrated (4 mg/kg) for 21 days. Environmental enrichment consisted of housing animals in large cages (50× 50× 50 cm) containing a running wheel and differently shaped objects for 21 days. Spatial learning and memory were assessed in the Morris water maze (MWM) and Real-time PCR was performed to assess the expression of brain-derived neurotrophic factor (BDNF) and M1 muscarinic acetylcholine receptor (AchM1R) within the hippocampus. Spatial memory was impaired in AD animals, and while neither pretreatment with donepezil nor EE alone could significantly restore spatial memory scores in AD rats, combination therapy was effective. BDNF expression was suppressed in AD rats and pretreatment with donepezil plus EE could increase it to the saline levels. The data suggest that a cholinesterase inhibitor and cognitive stimulation can be used effectively in combination to improve cognitive loss in an AD rat model.

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“…Besides, oxidative stress, mediated by reactive oxygen species (ROS)-induced damage, plays a devastating role in stroke pathogenesis. Malondialdehyde (MDA) would accelerate the chain reaction of ROS formation and is used as a biomarker of the oxidative stress level corelated with cognitive function ( 15 ).…”

Section: Introductionmentioning

confidence: 99%

The Effects of Enriched Rehabilitation on Cognitive Function and Serum Glutamate Levels Post-stroke

et al. 2022

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AimThis study aimed to explore the effect of enriched rehabilitation (ER) on cognitive function and serum glutamate levels in patients with stroke.MethodsForty patients diagnosed with post-stroke cognitive impairment (PSCI), according to the inclusion criteria, and undergoing inpatient rehabilitation were enrolled in the study. Patients were randomly assigned to receive 8 weeks of ER treatment (ER group; n = 20) or conventional medical treatment (CM group; n = 20). In addition, 20 age-matched healthy subjects who were outpatients in our hospital during the same period formed the healthy control (HC) group. In- and between-group differences in cognitive function were assessed during pre-intervention and post-intervention based on the Montreal Cognitive Assessment (MoCA), the Symbol Digit Modalities Test (SDMT), and the Trail Making Test (TMT). The serum levels of glutamate, tumor necrosis factor (TNF), and malondialdehyde (MDA) levels were also detected pre-intervention and post-intervention.ResultsPre-intervention cognitive function and the levels of all the serum parameters assessed significant difference between the HC group and the PSCI group (both ER and CM groups) (p < 0.05), but not between the two groups of patients with PSCI (p > 0.05). Significant improvements were observed in cognitive function in both the ER and the CM groups post-intervention compared with pre-intervention, as evidenced by the measured improvement in MoCA, SDMT, and TMT scores. Similar improvements were seen for serum glutamate, the degree of oxidative damage, and the level of inflammation in both the treatment groups (p < 0.05). More enhancements in cognitive function, including MoCA, SDMT, TMT scores, and the serum levels of glutamate, the degree of oxidative damage, and the level of inflammation were shown in the ER group compared with the CM group post-intervention (p < 0.05).ConclusionsER can improve cognitive function in patients with PSCI. The associated mechanism may be related to the negative regulatory effect of ER on serum glutamate, TNF, and MDA levels, which is likely to enhance synaptic plasticity and alleviate oxidative stress- and inflammation-related damage, at least to some extent.

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Comparison of malondialdehyde levels and superoxide dismutase activity in resveratrol and resveratrol/donepezil combination treatment groups in Alzheimer’s disease induced rat model

Cited by 35 publications

References 41 publications

Study on the Effects of Chinese Materia Medica Processing on the Hypoglycemic Activity and Chemical Composition of Anemarrhenae Rhizoma

Study on the Effects of Chinese Materia Medica Processing on the Hypoglycemic Activity and Chemical Composition of Anemarrhenae Rhizoma

Combination Therapy of Donepezil and Environmental Enrichment on Memory Deficits in Amyloid-Beta-Induced Alzheimer's Disease Rats

The Effects of Enriched Rehabilitation on Cognitive Function and Serum Glutamate Levels Post-stroke

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