Comparison of Manuka, Kanuka, and Black Locust Honey on the Production of Chemical Mediators by Peripheral Blood Mononuclear Cells

Shin, Seung Heon; Ye, Mi Kyung; Son, Ho Jin; Kim, Yee-Hyuk

doi:10.18787/jr.2017.24.2.104

Cited by 2 publications

(1 citation statement)

References 15 publications

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“…Consistent with the incomplete destruction of LPS, gamma irradiation doesn’t neutralise the effect of honey on cytokine secretion. Shin et al 33 reported a dose-dependent secretion of TNF-α and IL-10 by cultured peripheral blood mononuclear cells that were exposed to honey samples that were sterilized with 25 kGy gamma irradiation. Interestingly, LPS-induced secretion of interferon gamma, but not TNF-α, was suppressed by pre-treatment of cells with manuka and kanuka honeys.…”

Section: Discussionmentioning

confidence: 99%

Secretion of IL-6 by fibroblasts exposed to Australian honeys involves lipopolysaccharide and is independent of floral source

Russell

Visagie

Noll

2022

Sci Rep

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Honey stimulates cellular secretion of cytokines, which has been attributed to activation of lipopolysaccharide (LPS)-dependent and LPS-independent pathways. The objective of this study was to identify whether LPS is present in Australian honey samples at levels that can stimulate interleukin-6 (IL-6) secretion by fibroblasts and whether it can transduce cell signalling by activating toll-like receptor 4 (TLR4). IL-6 was measured in culture media of fibroblasts exposed to honey for 24 h. LPS was detected in a 0.125 mg/mL solution of grey ironbark honey (0.61 ± 0.05 ng/g honey). TLR4 signalling was observed in RAW264.7 macrophages that were exposed to honey and this was prevented by preincubating the honey with the LPS-neutralising agent, polymyxin B. Australian Eucalyptus, Leptospermum and Cyathode honeys stimulated IL-6 secretion in cultured human dermal fibroblasts. To examine whether the response was dependent on floral source, fibroblasts were exposed to four different samples of grey ironbark honey obtained from Queensland and New South Wales, Australia. The magnitude of the cytokine response to these honeys was highly varied. We conclude that Australian honeys contain endotoxin at levels that can stimulate IL-6 secretion by fibroblasts and that signalling in macrophages involves TLR4 activation. The IL-6 secretory response was independent of floral source.

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Section: Discussionmentioning

confidence: 99%