Comparison of Matrigel™ and gelatin substrata for feeder-free culture of undifferentiated mouse embryonic stem cells for toxicity testing

Greenlee, Anne R.; Kronenwetter-Koepel, Tamara A.; Kaiser, Steven J; Liu, K.

doi:10.1016/j.tiv.2004.11.002

Cited by 26 publications

(16 citation statements)

References 31 publications

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“…Greenlee et al reported that mouse ES cells cultured on gelatin spread well and form flat colonies with occasional round and raised appearance [49]. In the present study too, the iPS cells on Collagen PS showed the highest cell number at 5 d (Fig.…”

supporting

confidence: 61%

“…2). Collagen or gelatin is a popular matrix for general cell culture and for the culture of ES cells [11,43,48,49] and iPS cells [11,12] under feeder-free conditions. The highest number of adherent mouse iPS cells on Collagen PS recorded in this study was in agreement with that indicating highly efficient adhesion of human ES cells on a collagen-coated surface [48].…”

Section: Discussionmentioning

confidence: 99%

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Maintenance of hemiround colonies and undifferentiated state of mouse induced pluripotent stem cells on carbon nanotube-coated dishes

Akasaka

Yokoyama

Matsuoka

et al. 2011

Carbon

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Induced pluripotent stem (iPS) cells have attracted worldwide interest. However, there have been only a few studies investigating effective culture substrates for feeder-free culturing for the maintenance of iPS cells. In this study, we cultured mouse iPS cells under feeder-free conditions on carbon nanotube (CNT)-coated dishes and then evaluated the colony morphology and differentiation state of the cells on the dishes.After 5 d of cultivation in a medium containing 15% fetal bovine serum (FBS) and leukemia inhibitory factor (LIF), the colonies on thick films of multi-walled CNTs (MWCNTs) were observed to be hemiround; further, the cells expressed early undifferentiation markers. On the other hand, the colonies on a cell culture polystyrene dish and a collagen-coated polystyrene dish showed indistinct outline and spread well, and most spreading cells only weakly expressed early undifferentiation markers. These results indicate that the thick films of MWCNTs could maintain hemiround colonies and undifferentiated state of mouse iPS cells cultured under feeder-free conditions.

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supporting

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Maintenance of hemiround colonies and undifferentiated state of mouse induced pluripotent stem cells on carbon nanotube-coated dishes

Akasaka

Yokoyama

Matsuoka

et al. 2011

Carbon

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“…Consistent with the inhibitory effects of the BM on the progression of cell differentiation, mouse and human ES cells adhering to Matrigel TM or laminin stably retain their undifferentiated state, whereas those adhering to other substrates, such as fibronectin, do not (29,30). Furthermore, cell-BM interactions have been shown to maintain the differentiation status of BM-adherent cells in other tissues.…”

Section: Lamc1mentioning

confidence: 60%

Regulation of Mesodermal Differentiation of Mouse Embryonic Stem Cells by Basement Membranes

Fujiwara

Hayashi

Sanzen

et al. 2007

Journal of Biological Chemistry

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Basement membranes (BMs) have been implicated in cell fate determination during development. Embryoid bodies (EBs) derived from mouse embryonic stem cells deficient in the laminin ␥1 chain are incapable of depositing a BM, resulting in failure of primitive ectoderm epithelialization. To elucidate the mechanisms involved in this phenomenon, we compared the gene expression profiles of EBs with or without a BM to identify the genes showing BM-dependent expression. We found that the expressions of marker genes for the epithelial-mesenchymal transition (EMT), including the transcription factor Snai2, were up-regulated in LAMC1 ؊/؊ EBs, whereas restoration of a BM to LAMC1 ؊/؊ EBs suppressed the up-regulation of these genes. Overexpression of Snai2 induced the EMT in control EBs by molecular and morphological criteria, suggesting that suppression of the EMT regulatory genes is involved in BM-dependent epithelialization of primitive ectoderm. Despite the failure of primitive ectoderm epithelialization in BM-deficient EBs, mesodermal differentiation was not compromised, but rather accelerated. Furthermore, at later stages of control EB differentiation, the BM was disrupted at the gastrulation site where mesodermal markers were strongly expressed only in cells that had lost contact with the BM. Taken together, these results indicate that the BM prevents the EMT and precocious differentiation of primitive ectoderm toward mesoderm in EBs, implying that BMs are important for the control of mammalian gastrulation.

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“…We note that other approaches, both based on matrices [5,41] and molecular [36,38] approaches have been presented. The matrix approach described here is particularly flexible, and moreover may be useful in investigating Fig.…”

Section: Discussionmentioning

confidence: 99%

Engineering integrin signaling for promoting embryonic stem cell self-renewal in a precisely defined niche

et al. 2010

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Comparison of Matrigel™ and gelatin substrata for feeder-free culture of undifferentiated mouse embryonic stem cells for toxicity testing

Cited by 26 publications

References 31 publications

Maintenance of hemiround colonies and undifferentiated state of mouse induced pluripotent stem cells on carbon nanotube-coated dishes

Maintenance of hemiround colonies and undifferentiated state of mouse induced pluripotent stem cells on carbon nanotube-coated dishes

Regulation of Mesodermal Differentiation of Mouse Embryonic Stem Cells by Basement Membranes

Engineering integrin signaling for promoting embryonic stem cell self-renewal in a precisely defined niche

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