Comparison of MB-Check, BACTEC, and egg-based media for recovery of mycobacteria

Abe, Chiyoji; Hosojima, S; Fukasawa, Yutaka; Kazumi, Yuko; Takahashi, Machiko; Hirano, Kazuyuki; Mori, Toru

doi:10.1128/jcm.30.4.878-881.1992

Cited by 92 publications

(59 citation statements)

References 10 publications

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“…Two hundred and fifty patients were recruited into 22 treatment arms with either experimental compounds (TMC207, OPC-67683, PA-824) or control regimens (H, R or HRZE). Median group size was 12 subjects (mean, 11.4; range, [6][7][8][9][10][11][12][13][14][15][16]. Sputum was sampled at baseline before drug treatment and daily or every 2 days thereafter.…”

Section: Patients and Sputum Samplesmentioning

confidence: 99%

Time to liquid culture positivity can substitute for colony counting on agar plates in early bactericidal activity studies of antituberculosis agents

Diacon

Maritz

Venter

et al. 2012

Clinical Microbiology and Infection

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The measurement of early bactericidal activity (EBA) is the first step in the clinical investigation of antituberculosis agents. EBA is determined by quantifying the viable sputum mycobacterial load on consecutive days of treatment. To investigate whether time to positivity (TTP) in mycobacterial liquid culture can substitute for colony forming unit (CFU) counting on agar plates we compared the error variation of TTP and CFU in 2115 pooled sputum samples collected overnight from 250 individuals included in five EBA studies. We found that the technical variation between duplicate laboratory measurements and the within-subject or day-to-day variation were similar for TTP (8.5% and 27.4% of total variation, respectively) and CFU (6.7% and 29.3% of total variation). The ability of the measurements to separate the EBA of 22 treatment arms was determined with group rank correlation of means and one-way analysis of variance. Except for the EBA over 0-2 days, individual and group EBAs measured with TTP and CFU were highly correlated. Treatment group means rank correlation coefficients were r=0.472, r=0.910 and r=0.818, respectively, for EBA 0-2 days, EBA 0-7 days and EBA 0-14 days. Analysis of variance significantly favoured TTP over CFU for discrimination between groups with F values of 6.58 and 1.87, 7.77 and 4.58, and 8.71 and 3.56, respectively. We conclude that TTP is an acceptable alternative to CFU counting for the determination of the viable sputum mycobacterial load in EBA studies of up to 14 days duration.

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Section: Patients and Sputum Samplesmentioning

confidence: 99%

Time to liquid culture positivity can substitute for colony counting on agar plates in early bactericidal activity studies of antituberculosis agents

Diacon

Maritz

Venter

et al. 2012

Clinical Microbiology and Infection

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“…Detection by microscopy is useful as a rapid screening test, but its sensitivity is low (10). Culture on solid medium can take up to 8 weeks to yield a negative result (1,13). The radiometric BACTEC 460 TB system (Becton Dickinson Diagnostic Instrument Systems, Sparks, Md.)…”

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confidence: 99%

“…The radiometric BACTEC 460 TB system (Becton Dickinson Diagnostic Instrument Systems, Sparks, Md.) has been an important addition to culture methods; however, this technique requires an average of 13 to 15 days to detect positive specimens (1,13,19).…”

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Evaluation of the semiautomated Abbott LCx Mycobacterium tuberculosis assay for direct detection of Mycobacterium tuberculosis in respiratory specimens

et al. 1997

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Five hundred twenty processed respiratory specimens from 326 patients received for the diagnosis of tuberculosis or other mycobacterial infections were tested by means of the LCx Mycobacterium tuberculosis Assay from Abbott Laboratories, which uses ligase chain reaction technology for the direct detection of M. tuberculosis complex in respiratory specimens. The results of the LCx M. tuberculosis Assay were compared with the results of culture and staining techniques. After a combination of culture results and the patient's clinical data, a total of 195 specimens were collected from 110 patients who were positively diagnosed as having pulmonary tuberculosis. Twenty-three of these 195 specimens which corresponded to 10 patients with a history of pulmonary tuberculosis (TB) and anti-TB treatment ranging from 1 to 6 months were culture negative. The other 172 specimens were culture positive for M. tuberculosis. With an overall positivity rate of 37.5% (195 of 520 specimens), the sensitivity, specificity, and positive and negative predictive values were 90.8, 100, 100, and 94.7%, respectively, for the LCx M. tuberculosis Assay; 88.2, 100, 100, and 93.4%, respectively, for culture; and 82.6, 92, 72.9, and 97.6%, respectively, for acid-fast staining. For 161 specimens (82.6%) from patients smear positive for the disease and 34 specimens (17.4%) from patients smear negative for the disease, the sensitivity values for the LCx M. tuberculosis Assay were 98.8 and 53%, respectively. There were no statistically significant differences in the sensitivities and specificities between the LCx M. tuberculosis Assay and culture (P > 0.05). Conclusively, the LCx M. tuberculosis Assay has proved to have an acceptable sensitivity and a high specificity in detecting M. tuberculosis and has the potential of reducing the diagnosis time to an 8-h working day.Semiquantitative reporting modified from a previous report (22); 0, no acid-fast bacilli seen; 1ϩ, a few acid-fast bacilli seen; 2ϩ, moderate numbers of acid-fast bacilli seen; 3ϩ, many acid-fast bacilli seen. c

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“…Traditional methods for isolating M. tuberculosis from human specimens by culture can take up to 8 weeks. A radiometric method (BACTEC 460 TB) has reduced the culture time to less than 2 weeks (1,2,7,8). More recently, a new, fully automated, nonradiometric method, fluorescent BACTEC 9000 MB (Becton Dickinson), which uses an oxygen-quenched fluorescence indicator for the rapid detection of Mycobacterium spp., has been introduced.…”

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Evaluation of a nonradiometric system (BACTEC 9000 MB) for detection of mycobacteria in human clinical samples

et al. 1997

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This study was carried out to evaluate the rate of recovery and time required for detection of mycobacteria from pulmonary and extrapulmonary human clinical samples, by using a fluorescence-quenching-based oxygen sensor (BACTEC 9000 MB; Becton Dickinson Microbiology Systems, Sparks, Md.). The results were compared with those obtained by microscopy, conventional culture in Lowenstein-Jensen (LJ) medium, and a BACTEC radiometric system (BACTEC 460 TB; Becton Dickinson). Of the 779 clinical samples processed, 364 from pulmonary sites and 415 from extrapulmonary sites, 62 (7.9%) were positive for mycobacterial isolates; of the positive samples, 59 (95.1%) were detected with the fluorescent BACTEC 9000 MB system, 57 (91.9%) were detected with the radiometric system (BACTEC 460 TB), and 43 (69.3%) were detected with LJ conventional culture. The mean times to detection of all mycobacteria with BACTEC 9000 MB and BACTEC 460 TB were similar (10.3 and 10.0 days, respectively). The results obtained indicate that the nonradiometric BACTEC (BACTEC 9000 MB) system is as efficient as Bactec 460 TB and significantly more efficient than LJ for the rapid recovery of mycobacteria from both pulmonary and extrapulmonary clinical specimens. Though the BACTEC 9000 MB system is recommended for respiratory specimens, we demonstrated that it can be successfully used also for recovery of mycobacteria from clinical specimens from various extrapulmonary sites.

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Comparison of MB-Check, BACTEC, and egg-based media for recovery of mycobacteria

Cited by 92 publications

References 10 publications

Time to liquid culture positivity can substitute for colony counting on agar plates in early bactericidal activity studies of antituberculosis agents

Time to liquid culture positivity can substitute for colony counting on agar plates in early bactericidal activity studies of antituberculosis agents

Evaluation of the semiautomated Abbott LCx Mycobacterium tuberculosis assay for direct detection of Mycobacterium tuberculosis in respiratory specimens

Evaluation of a nonradiometric system (BACTEC 9000 MB) for detection of mycobacteria in human clinical samples

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