Comparison of Measurements of 12 Analytes in Equine Blood Samples Using the In-Practice Falcor 350 and the Reference KoneLab 30i Analysers

Abstract

Falcor 350 is a wet-reagent biochemistry analyser that is available for in-house use. The aim of this study was to compare the results produced by this analyser with those obtained by the KoneLab 30i that served as the reference instrument. Blood samples from 60 clinical cases were analysed for urea, creatinine, total proteins, albumin, creatine kinase, aspartate aminotransferase, alkaline phosphatase, total bilirubin, total calcium, phosphate, sodium, and potassium using both instruments. Good to excellent correlations (r s value) value) were identified for creatinine (0.88), total proteins (0.92), albumin (0.93), creatine kinase (0.98), aspartate aminotransferase (0.98), alkaline phosphatase (0.94), total bilirubin (0.98), phosphate (0.95), and potassium (0.97). The correlations for total calcium (0.71), sodium (0.68), and urea (0.64) were fair. For albumin, aspartate aminotransferase, creatine kinase, phosphate, potassium, total bilirubin, creatinine, and total proteins, the two instruments produce values that are closely related to each other and are sufficiently similar to allow them to be used interchangeably without the need for additional correction factor computations. Because of differences in the methodologies, the Falcor results for alkaline phosphatase, total calcium, and sodium cannot be used interchangeably and should be interpreted using reference intervals established from the Falcor analyser.

Effect of storage temperature and time on measurement of serum symmetric dimethylarginine concentration using point‐of‐care and commercial laboratory analyzers in cats and dogs

“…However, the observed meaningful bias and wide LoA's in the BA plots confirmed that interchangeable use of the POC and CL analyzers to trend serial results in a particular individual is not recommended for sSDMA. This finding is consistent with several other analytes for which bias between analyzers has been shown 9,38‐40 . The observed bias of sSDMA between analyzers may impact the use of RCV (ie, percentage increase or decrease between serial measurements representing clinically relevant biological change 18 ) or critical difference (ie, conversion of RCV in measured units 37 ).…”

“…This finding is consistent with several other analytes for which bias between analyzers has been shown. 9,[38][39][40] The observed bias of sSDMA between analyzers may impact the use of RCV (ie, percentage increase or decrease between serial measurements representing clinically relevant biological change 18 ) or critical difference (ie, conversion of RCV in measured units 37 ). Because the observed bias tends to be proportional it becomes more important with sSDMA concentrations exceeding the RI to use the same analyzer during follow-up.…”

Initial analytic quality assessment and method comparison of an immunoassay for adrenocorticotropic hormone measurement in equine samples

“…Major advances in diagnostic technology have allowed equine clinics to perform laboratory analyses in house. These are most commonly performed using bench‐top hematology and biochemistry analyzers, which aid in the diagnosis of various diseases by generating results immediately …”