Comparison of mechanisms of hexadecane uptake among pure and mixed cultures derived from a bacterial consortium

Tzintzun-Camacho, Olivia; Loera, Octavio; Ramírez-Saad, Hugo; Gutiérrez-Rojas, M.

doi:10.1016/j.ibiod.2012.01.009

Cited by 48 publications

(21 citation statements)

References 38 publications

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“…Comparison of transcriptomic data obtained from all three carbon source additions explicated the activity of these genes during hexadecane degradation. Emulsification and membrane genes that are known to assist in the degradation of hydrophobic alkanes (Tzintzun-Camacho et al, 2012) were both present and active within Smithella. The gene encoding an apolipoprotein N-acyltransferase is of particular note, as previous studies have shown that deletion of lipoprotein synthesis genes can decrease membrane hydrophobicity in bacteria (Okugawa et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Single-cell genome and metatranscriptome sequencing reveal metabolic interactions of an alkane-degrading methanogenic community

et al. 2013

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Microbial interactions have a key role in global geochemical cycles. Although we possess significant knowledge about the general biochemical processes occurring in microbial communities, we are often unable to decipher key functions of individual microorganisms within the environment in part owing to the inability to cultivate or study them in isolation. Here, we circumvent this shortcoming through the use of single-cell genome sequencing and a novel low-input metatranscriptomics protocol to reveal the intricate metabolic capabilities and microbial interactions of an alkane-degrading methanogenic community. This methanogenic consortium oxidizes saturated hydrocarbons under anoxic conditions through a thus-far-uncharacterized biochemical process. The genome sequence of a dominant bacterial member of this community, belonging to the genus Smithella, was sequenced and served as the basis for subsequent analysis through metabolic reconstruction. Metatranscriptomic data generated from less than 500 pg of mRNA highlighted metabolically active genes during anaerobic alkane oxidation in comparison with growth on fatty acids. These data sets suggest that Smithella is not activating hexadecane by fumarate addition. Differential expression assisted in the identification of hypothetical proteins with no known homology that may be involved in hexadecane activation. Additionally, the combination of 16S rDNA sequence and metatranscriptomic data enabled the study of other prevalent organisms within the consortium and their interactions with Smithella, thus yielding a comprehensive characterization of individual constituents at the genome scale during methanogenic alkane oxidation.

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Section: Discussionmentioning

confidence: 99%

Single-cell genome and metatranscriptome sequencing reveal metabolic interactions of an alkane-degrading methanogenic community

et al. 2013

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“…This can be facilitated by exposing the hydrophilic or hydrophobic moieties of cell-bound biosurfactants external to the cell (Rosenberg et al, 1988). Microorganisms with degradation capabilities may also alter their cell hydrophobicity during growth on PHCs (Franzetti et al, 2008a; Tzintzun-Camacho et al, 2012). …”

Section: Bioremediation Of Petroleum Hydrocarbonsmentioning

confidence: 99%

The Interaction between Plants and Bacteria in the Remediation of Petroleum Hydrocarbons: An Environmental Perspective

et al. 2016

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Widespread pollution of terrestrial ecosystems with petroleum hydrocarbons (PHCs) has generated a need for remediation and, given that many PHCs are biodegradable, bio- and phyto-remediation are often viable approaches for active and passive remediation. This review focuses on phytoremediation with particular interest on the interactions between and use of plant-associated bacteria to restore PHC polluted sites. Plant-associated bacteria include endophytic, phyllospheric, and rhizospheric bacteria, and cooperation between these bacteria and their host plants allows for greater plant survivability and treatment outcomes in contaminated sites. Bacterially driven PHC bioremediation is attributed to the presence of diverse suites of metabolic genes for aliphatic and aromatic hydrocarbons, along with a broader suite of physiological properties including biosurfactant production, biofilm formation, chemotaxis to hydrocarbons, and flexibility in cell-surface hydrophobicity. In soils impacted by PHC contamination, microbial bioremediation generally relies on the addition of high-energy electron acceptors (e.g., oxygen) and fertilization to supply limiting nutrients (e.g., nitrogen, phosphorous, potassium) in the face of excess PHC carbon. As an alternative, the addition of plants can greatly improve bioremediation rates and outcomes as plants provide microbial habitats, improve soil porosity (thereby increasing mass transfer of substrates and electron acceptors), and exchange limiting nutrients with their microbial counterparts. In return, plant-associated microorganisms improve plant growth by reducing soil toxicity through contaminant removal, producing plant growth promoting metabolites, liberating sequestered plant nutrients from soil, fixing nitrogen, and more generally establishing the foundations of soil nutrient cycling. In a practical and applied sense, the collective action of plants and their associated microorganisms is advantageous for remediation of PHC contaminated soil in terms of overall cost and success rates for in situ implementation in a diversity of environments. Mechanistically, there remain biological unknowns that present challenges for applying bio- and phyto-remediation technologies without having a deep prior understanding of individual target sites. In this review, evidence from traditional and modern omics technologies is discussed to provide a framework for plant–microbe interactions during PHC remediation. The potential for integrating multiple molecular and computational techniques to evaluate linkages between microbial communities, plant communities and ecosystem processes is explored with an eye on improving phytoremediation of PHC contaminated sites.

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“…Aunque en hongos no existen reportes sobre la producción de biosurfactantes, existe la capacidad de ciertas especies fúngicas para producir proteínas de bajo peso molecular con actividad surfactante llamadas hidrofobinas (Cox y Hooley 2009). Este tipo de adaptación permite la adhesión de las gotas de hidrocarburos, lo que facilita la difusión de los sustratos hidrofóbicos al interior de las células microbianas (Bouchez 2001, Van Hamme 2001, Abbasnezhad 2011, Tzintzun-Camacho et al 2012.…”

Section: Resultados Y Discusiónunclassified

“…Los modelos matemáticos de crecimiento son empleados para describir el comportamiento de microorganismos en diferentes condiciones quími-cas, tales como temperatura o pH (Zwietering et al 1990) a través de datos de sólidos suspendidos por peso seco (Lizardi-Jiménez et al 2013) o unidades formadoras de colonias (Tzintzun-Camacho et al 2012). Sin embargo, en muchas de las aplicaciones la espectrofotometría es usada como un método directo y sencillo para conocer el comportamiento de un sistema biológico, aunque existen escasos reportes de su ajuste sobre modelos matemáticos en bioprocesos.…”

Section: Introductionunclassified

Aislamiento Y Análisis Cualitativo De Biomasa Microbiana Fúngica Degradadora De Hidrocarburos De Un Cenote De Quintana Roo

Gómez-Reyes

Medina-Moreno

Jiménez-González

et al. 2017

Rev. Int. Contam. Ambie.

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Palabras clave: hongos, biorremediación, petróleo, consumo por contacto directo RESUMENSe cultivó en biorreactores de puente aéreo un consorcio microbiano para estudios ex situ. El consorcio fue obtenido de un cenote contaminado con fenantreno y naftaleno ubicado en la ciudad de Cancún, Quintana Roo, México. En este trabajo se mostró que el consorcio microbiano está también compuesto por hongos con al menos una especie de Cladosporium, además de las bacterias previamente identificadas en trabajos anteriores del grupo de investigación (Pseudomonas, Diplococcus y Enterobacter), por lo que se conforma así un consorcio mixto. Se modeló la cinética de crecimiento de biomasa fúngica utilizando tres modelos: Gompertz, de Von Bertalanffy y logístico para los datos obtenidos por espectrofotometría. Para todos los casos, Von Bertalanffy fue el modelo que se ajustó de manera sobresaliente a los valores experimentales (σ ≤ 0.4). El análisis por estereomicroscopía contribuyó a dar una explicación cualitativa de los mecanismos de asimilación de hidrocarburo en biomasa fúngica, al hacer evidente el contacto directo entre esta biomasa fúngica y el hidrocarburo. Finalmente, cultivos en cajas petri recubiertas con petróleo crudo maya corroboraron que la biomasa fúngica obtenida, produce alguna clase de hidrofobinas que permiten romper la tensión superficial entre la interfase hidrofílica/hidrofóbica, favoreciendo así el contacto con el sustrato y los microorganismos.Key words: fungus, bioremediation, petroleum, direct contact uptake ABSTRACTMicrobial consortium has been cultured in airlift bioreactors for ex situ studies. Consortium was obtained in Cancun city from a naphthalene and phenanthrene polluted sinkhole. This work showed that the microbial consortium is further comprised of at least one species fungi (Cladosporium) additionally to bacteria identified in a previous work from our research group (Pseudomonas, Diplococcus and Enterobacter), forming thus a mixed consortium. Mathematical models were used to adjust biomass

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Comparison of mechanisms of hexadecane uptake among pure and mixed cultures derived from a bacterial consortium

Cited by 48 publications

References 38 publications

Single-cell genome and metatranscriptome sequencing reveal metabolic interactions of an alkane-degrading methanogenic community

Single-cell genome and metatranscriptome sequencing reveal metabolic interactions of an alkane-degrading methanogenic community

The Interaction between Plants and Bacteria in the Remediation of Petroleum Hydrocarbons: An Environmental Perspective

Aislamiento Y Análisis Cualitativo De Biomasa Microbiana Fúngica Degradadora De Hidrocarburos De Un Cenote De Quintana Roo

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