Comparison of Meconium Microbiome in Dizygotic and Monozygotic Twins Born by Caesarean Section (CS)

Yang, Jing; Yao, Su; Cheng, Kun; Xu, Lili; Hou, Lingling; Wei, Yuan; Feng, Huijun; Yu, Xuejian; Zhang, Xin; Tong, Xiao-Mei; Li, Zailing; Zhao, Yangyu

doi:10.3389/fmicb.2020.01139

Cited by 12 publications

(12 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, most preterm infants delivered by cesarean section have a higher abundance of Proteobacteria than do full-term infants (12,17,25,34), which is consistent with our finding that Proteobacteria was predominant during the T1 stage. It should be noted that our results differ from previous reports that the gut microbiome of newborns during early life had low diversity or was sterile (12,27,(37)(38)(39). Also, DNA extraction methods may affect the determination of microbiome diversity in low biomass samples, which should be further studied in future.…”

Section: Discussioncontrasting

confidence: 90%

Postnatal age is strongly correlated with the early development of the gut microbiome in preterm infants

Shen¹,

Qiu²,

Liu³

et al. 2021

Transl Pediatr

View full text Add to dashboard Cite

Background: The gut microbiome plays a potential role in clinical events in preterm infants and may affect their lateral development. Understanding the initial colonization of microbes in the gut, their early dynamic changes, and the major factors correlated with these changes would provide crucial information about the developmental process in early life.Methods: The present study enrolled 151 preterm infants and examined the longitudinal dynamics of their fecal microbiome profiles during the period of hospitalization using 16S ribosomal RNA gene sequencing.Random forest modeling was used to predict postnatal age (Age), postmenstrual age (PMA), and gestational age (GA), using gut microbiome features.Results: Principal coordinate analysis revealed that the gut microbiome of the preterm infants displayed an obvious time-dependent change pattern, which showed the strongest association with Age, followed by PMA, and a much weaker association with (GA). Random forest modeling further evidenced the time-dependent change pattern, with the Pearson's correlation coefficients between the actual values and the gut microbiomepredicted values being 0.68, 0.53, and 0.38 for postnatal, postmenstrual, and gestational age, respectively.The microbiome dynamism could be further divided into four Age stages, each with its own characteristic microbial taxa. The first 1-4 days (T1 stage) represented the meconium microbiome, with colonization of a high diversity of microbes before or during delivery. During 5-15 days (T2 stage), the gut microbiome of the preterm infants underwent a rapid turnover, in which microbial diversity declined, and stabilized afterward.Enterobacteriaceae, Enterococcaceae, Streptococcaceae, Staphylococcaceae, and Clostridiaceae were the major classes in the gut microbiome in the lateral stages of development (T3-T4 stage).Conclusions: Postnatal age, rather than the gestational age, is significantly correlated with the gut microbiome of preterm infants, suggesting that clinical interventions contribute more to the early dynamics of gut microbiome in preterm infants than the natural development of the gut.

show abstract

Section: Discussioncontrasting

confidence: 90%

Postnatal age is strongly correlated with the early development of the gut microbiome in preterm infants

Shen¹,

Qiu²,

Liu³

et al. 2021

Transl Pediatr

View full text Add to dashboard Cite

show abstract

“…Each infant had faecal samples from at least 3 days after birth. Sampling was conducted following a previously published protocol 14. Approximately 1–1.5 g faecal samples were sampled using a sterile plastic spoon and then placed in sterile tubes under strict aseptic conditions.…”

Section: Methodsmentioning

confidence: 99%

“…Twins, with their high genetic and environmental resemblance, are excellent model for disentangling the effect of genetic and environmental factors on shaping the gut microbiota. For example, a few twin studies have investigated the genetic determinants of the gut microbiota 14 15. However, knowledge on the impact of intrauterine environment on early-life gut microbiota remains largely unknown to date.…”

Section: Introductionmentioning

confidence: 99%

Unfavourable intrauterine environment contributes to abnormal gut microbiome and metabolome in twins

Yang

Hou

Wang

et al. 2022

Gut

Self Cite

View full text Add to dashboard Cite

ObjectiveFetal growth restriction (FGR) is a devastating pregnancy complication that increases the risk of perinatal mortality and morbidity. This study aims to determine the combined and relative effects of genetic and intrauterine environments on neonatal microbial communities and to explore selective FGR-induced gut microbiota disruption, metabolic profile disturbances and possible outcomes.DesignWe profiled and compared the gut microbial colonisation of 150 pairs of twin neonates who were classified into four groups based on their chorionicity and discordance of fetal birth weight. Gut microbiota dysbiosis and faecal metabolic alterations were determined by 16S ribosomal RNA and metagenomic sequencing and metabolomics, and the long-term effects were explored by surveys of physical and neurocognitive development conducted after 2~3 years of follow-up.ResultsAdverse intrauterine environmental factors related to selective FGR dominate genetics in their effects of elevating bacterial diversity and altering the composition of early-life gut microbiota, and this effect is positively related to the severity of selective FGR in twins. The influence of genetic factors on gut microbes diminishes in the context of selective FGR. Gut microbiota dysbiosis in twin neonates with selective FGR and faecal metabolic alterations features decreased abundances of Enterococcus and Acinetobacter and downregulated methionine and cysteine levels. Correlation analysis indicates that the faecal cysteine level in early life is positively correlated with the physical and neurocognitive development of infants.ConclusionDysbiotic microbiota profiles and pronounced metabolic alterations are associated with selective FGR affected by adverse intrauterine environments, emphasising the possible effects of dysbiosis on long-term neurobehavioural development.

show abstract

“…For example, recently it was reported that 97% of microbiome phenotypes in wild baboons were significantly heritable [142]. In humans, because the overall similarity of gut bacterial community composition between adult mono-and di-zygotic twin pairs is largely the same, it is reasonable to conclude that physical contact during and after birth has a greater influence as compared to genetic relatedness, in determining gut microbial composition [143,144]. Although it is not clear how much of the human gut microbiome is transferred from mother to infant, several groups of bacteria, such as specific strains of Bifidobacterium breve and Lactobacillus plantarum, were shown to be always present in the infant gut and their mothers' feces and milk [145][146][147], providing direct support for accurate vertical transmission of these strains.…”

Section: Physical Contactmentioning

confidence: 99%

Reconstitution and Transmission of Gut Microbiomes and Their Genes between Generations

Rosenberg

Zilber‐Rosenberg

2021

Microorganisms

View full text Add to dashboard Cite

Microbiomes are transmitted between generations by a variety of different vertical and/or horizontal modes, including vegetative reproduction (vertical), via female germ cells (vertical), coprophagy and regurgitation (vertical and horizontal), physical contact starting at birth (vertical and horizontal), breast-feeding (vertical), and via the environment (horizontal). Analyses of vertical transmission can result in false negatives (failure to detect rare microbes) and false positives (strain variants). In humans, offspring receive most of their initial gut microbiota vertically from mothers during birth, via breast-feeding and close contact. Horizontal transmission is common in marine organisms and involves selectivity in determining which environmental microbes can colonize the organism’s microbiome. The following arguments are put forth concerning accurate microbial transmission: First, the transmission may be of functions, not necessarily of species; second, horizontal transmission may be as accurate as vertical transmission; third, detection techniques may fail to detect rare microbes; lastly, microbiomes develop and reach maturity with their hosts. In spite of the great variation in means of transmission discussed in this paper, microbiomes and their functions are transferred from one generation of holobionts to the next with fidelity. This provides a strong basis for each holobiont to be considered a unique biological entity and a level of selection in evolution, largely maintaining the uniqueness of the entity and conserving the species from one generation to the next.

show abstract

Comparison of Meconium Microbiome in Dizygotic and Monozygotic Twins Born by Caesarean Section (CS)

Cited by 12 publications

References 54 publications

Postnatal age is strongly correlated with the early development of the gut microbiome in preterm infants

Postnatal age is strongly correlated with the early development of the gut microbiome in preterm infants

Unfavourable intrauterine environment contributes to abnormal gut microbiome and metabolome in twins

Reconstitution and Transmission of Gut Microbiomes and Their Genes between Generations

Contact Info

Product

Resources

About