Comparison of medetomidine, thiopental and ketamine/midazolam anesthesia in chick embryos for in ovo Magnetic Resonance Imaging free of motion artifacts

Waschkies, Conny; Nicholls, Flora; Buschmann, Johanna

doi:10.1038/srep15536

Cited by 19 publications

(25 citation statements)

References 24 publications

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“…While each imaging modality comes with advantages and limitations, MRI offers detailed three dimensional anatomical information and high resolution over time in a non-invasive manner. In agreement with others, we show here, that MRI is a powerful imaging modality for the study of tumour progression [18,19] and embryonic development [20][21][22][23][24] in the chick embryo. Compared to optical imaging, it can be used to detect the presence of tumours even when they are hidden beneath the egg shell and allows the non-invasive study of tumour progression and volume over time.…”

Section: Discussionsupporting

confidence: 93%

Magnetic resonance imaging for characterisation of a chick embryo model of cancer cell metastases

Herrmann

Taylor

Murray

et al. 2017

Preprint

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Section: Discussionsupporting

confidence: 93%

Magnetic resonance imaging for characterisation of a chick embryo model of cancer cell metastases

Herrmann

Taylor

Murray

et al. 2017

Preprint

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“…Volatile anesthetics decrease endothelium-dependent vasorelaxation through the nitric oxide signaling pathway, but also injectable anesthetics have been shown to inhibit vasoconstriction to various degrees. Our anesthesia protocol, optimized in our lab previously for the chicken embryo in ovo 37 , might also affect our observations with regards to changes in T2* and T1. However, imaging requires immobilization of the chicken embryo and corresponding confounding effects cannot entirely be precluded.…”

Section: Discussionmentioning

confidence: 99%

Tumor grafts grown on the chicken chorioallantoic membrane are distinctively characterized by MRI under functional gas challenge

Waschkies

Pfiffner

Heuberger

et al. 2020

Sci Rep

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Recently, a tumor model based on the chorioallantoic membrane (cAM) was characterized structurally with Magnetic Resonance imaging (MRi). Yet, capability of MRi to assess vascular functional reserve and potential of oxygenation-sensitive MRi remain largely unexplored in this model. for this purpose, we compared MC-38 colon and A549 lung adenocarcinoma cell grafts grown on the CAM, using quantitative T1 and T2* MRi readouts as imaging markers. these are associated with vascular functionality and oxygenation status when compared between periods of air and carbogen exposure. our data show that in A549 lung adenocarcinoma cell grafts T2* values increased significantly upon carbogen exposure (p < 0.004, Wilcoxon test; no change in T1), while MC-38 grafts displayed no changes in T1 and T2*), indicating that the grafts differ in their vascular response. Heterogeneity with regard to T1 and T2* distribution within the grafts was noted. MC-38 grafts displayed larger T1 and T2* in the graft centre, while in A549 they were distributed more towards the graft surface. Finally, qualitative assessment of gadolinium-enhancement suggests that A549 grafts display more prominent enhancement compared to MC-38 grafts. Furthermore, MC-38 grafts had 65% larger volumes than A549 grafts. Histology revealed distinct underlying phenotypes of the two tumor grafts, pertaining to the proliferative status (Ki-67) and cellularity (H&E). In sum, a functional gas challenge with carbogen is feasible through gas exchange on the CAM, and it affects MRI signals associated with vascular reactivity and oxygenation status of the tumor graft planted on the CAM. Different grafts based on A549 lung adenocarcinoma and MC-38 colon carcinoma cell lines, respectively, display distinct phenotypes that can be distinguished and characterized non-invasively in ovo using MRi in the living chicken embryo. The chorioallantoic membrane (CAM) of the developing chicken embryo is an established model that is used in biomedical research in a multitude of different applications 1. For instance, it is employed in screening biomaterials 2-4 , testing microsurgical procedures 5 , drug delivery systems and biosensors 6,7 , and in toxicity and pharmacokinetic studies 8,9. Recently, the CAM model was used to asses perfusion capacities of on-planted biomaterials with Magnetic Resonance Imaging (MRI) as a non-destructive imaging readout 10. The CAM serves as a support for the respiratory capillaries outside the embryo. It is highly vascularized and allows for gas exchange between the embryo and its environment. This renders the CAM a suitable model to study angiogenesis 11-14. Notably, as a natural immunodeficient host with a rich vascular network, the CAM is particularly capable to sustain grafted tissues and implants for tissue engineering applications 15. Most importantly, it provides an advantageous environment for tumor formation and is therefore often used to study tumor development, metastasis and progression in xenotransplanted tumors 16. Another advantage is the easy acces...

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“…Briefly, eggs were sedated with 0.3 mL of 0.3 mg/kg medetomidine (Dorbene ad us. vet., Injektionslösung, Dr. E. Graeub AG, Bern BE, Switzerland) 60 . T 1 -weighted MR images were acquired with a RARE sequence of variable TR and TE for quantitative T 1 and T 2 mapping (TR 200/400/800/1500/3000/4500 ms, TE 10/30/50/70/90 ms, RARE-factor 2, image matrix size 220 × 150, field of view 45 × 30 mm 2 , spatial resolution 0.2 × 0.2 mm 2 , slice thickness 1 mm, total scan time 9 min 40 s).…”

Section: Methodsmentioning

confidence: 99%

Novel multimodal MRI and MicroCT imaging approach to quantify angiogenesis and 3D vascular architecture of biomaterials

Woloszyk

Wolint

Becker

et al. 2019

Sci Rep

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Quantitative assessment of functional perfusion capacity and vessel architecture is critical when validating biomaterials for regenerative medicine purposes and requires high-tech analytical methods. Here, combining two clinically relevant imaging techniques, (magnetic resonance imaging; MRI and microcomputed tomography; MicroCT) and using the chorioallantoic membrane (CAM) assay, we present and validate a novel functional and morphological three-dimensional (3D) analysis strategy to study neovascularization in biomaterials relevant for bone regeneration. Using our new pump-assisted approach, the two scaffolds, Optimaix (laminar structure mimicking entities of the diaphysis) and DegraPol (highly porous resembling spongy bone), were shown to directly affect the architecture of the ingrowing neovasculature. Perfusion capacity (MRI) and total vessel volume (MicroCT) strongly correlated for both biomaterials, suggesting that our approach allows for a comprehensive evaluation of the vascularization pattern and efficiency of biomaterials. Being compliant with the 3R-principles (replacement, reduction and refinement), the well-established and easy-to-handle CAM model offers many advantages such as low costs, immune-incompetence and short experimental times with high-grade read-outs when compared to conventional animal models. Therefore, combined with our imaging-guided approach it represents a powerful tool to study angiogenesis in biomaterials.

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Comparison of medetomidine, thiopental and ketamine/midazolam anesthesia in chick embryos for in ovo Magnetic Resonance Imaging free of motion artifacts

Cited by 19 publications

References 24 publications

Magnetic resonance imaging for characterisation of a chick embryo model of cancer cell metastases

Magnetic resonance imaging for characterisation of a chick embryo model of cancer cell metastases

Tumor grafts grown on the chicken chorioallantoic membrane are distinctively characterized by MRI under functional gas challenge

Novel multimodal MRI and MicroCT imaging approach to quantify angiogenesis and 3D vascular architecture of biomaterials

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