Comparison of media used for the primary isolation of Mycobacteriurm bovis by veterinary and medical diagnostic laboratories

La, Corner; Nicolacopoulos, C

doi:10.1111/j.1751-0813.1988.tb14457.x

Cited by 22 publications

(18 citation statements)

References 6 publications

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“…The number of isolates and colonies were higher on B83, ST and LJp, confirming findings of previous studies (CORNER ;NICOLACOPOULOS, 1988;COUSINS et al, 1989). According to our data, seven M. bovis isolates grew only on ST, five only on LJp and four only on B83.…”

Section: Discussionsupporting

confidence: 81%

“…B83 and ST exhibited similar results in terms of faster detection, which supports Corner's (1994) data from studies using 491 samples, though other investigations showed lower time to first appearance of colonies on 7H11 (COUSINS et al, 1989;CORNER et al, 2012) and Middlebrook 7H11 medium with addition of antibiotics (GALLAGHER;HORWILL, 1977;CORNER;NICOLACOPOULOS, 1988).…”

Section: Discussionsupporting

confidence: 75%

“…Although solid media assays on this matter are rare, some studies were performed under incubation in air containing CO 2 (GALLAGHER; HORWILL, 1977;CORNER;NICOLACOPOULOS, 1988;CORNER;TRAJSTMAN, 1988;CORNER, 1994), despite no agreement or consistence on the concentration applied.…”

Section: Discussionmentioning

confidence: 99%

“…Since M. bovis field strains require enriched media for growth on primary isolation and minimal toxicity of decontaminant reagents (CORNER, 1994), there are several studies seeking the best performance medium (SCHAEFER, 1952;BIRN, 1965;GALLAGHER;HORWILL, 1977;CORNER;NICOLACOPOULOS, 1988;COUSINS et al, 1989), as well as for the best decontamination method (CORNER; TRAJSTMAN, 1988;AMBROSIO et al, 2008;CORNER et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Influence of the incubation conditions on culture media to optimize primary isolation of Mycobacterium bovis

Ikuta

Morato

Souza

et al. 2016

SCA

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The isolation of Mycobacterium bovis is critical to a surveillance system for bovine tuberculosis based on detection of lesions in abattoirs. Thus, four solid culture media and three incubation conditions were investigated to elucidate which combination overcomes the others by assessing growth, time to the first appearance of colonies and their number. Ninety-seven samples of granulomatous lesions were submitted to the decontamination procedure by 1-hexadecylpyridinium chloride at 0.75% w/v, and inoculated on two egg-based media, Stonebrink's (ST) and Löwenstein-Jensen's with sodium pyruvate (LJp), and two agar-based media, tuberculosis blood agar (B83) and Middlebrook 7H11 medium (7H11). Each medium was incubated at 37°C for 90 days in three incubation conditions: in air, in air containing 10% carbon dioxide (CO 2 ), and in air in slopes closed with burned hydrophobic cotton and subsequently plugged with a cork to create a microaerophilic atmosphere. The colonies appeared faster and in higher number when incubated in air containing 10% CO 2 (p < 0.01), independent of media. B83 showed a faster growth and detected more isolates at 30 days of incubation, when compared to ST (0.0178), LJp (p < 0.0001) and 7H11 (p < 0.0001), though there was no difference between B83, ST and LJp at 60 and 90 days of incubation. 7H11 presented the lowest number of isolates (p < 0.0001) and a longer period for the appearance of the first colony (p < 0.001). According to our findings, the concomitant use of ST and B83 media incubated in air containing 10% CO 2 increases the isolation of M. bovis in a shorter period of time, which improves bovine tuberculosis diagnosis. Key words: Primary isolation. Bovine tuberculosis. Mycobacterium bovis. Culture media. Incubation conditions. ResumoO isolamento do Mycobacterium bovis é fundamental para um sistema de vigilância para tuberculose bovina baseado na detecção de lesões em abatedouro. Assim, quatro meios de cultura sólidos e três condições de incubação foram investigados para elucidar qual combinação supera as outras através da avaliação de crescimento, tempo para o aparecimento da primeira colônia e número de colônias. Noventa e sete amostras de lesões granulomatosas foram submetidas ao processo de descontaminação por cloreto de 1-hexadecilpiridínio a 0,75%, e inoculadas em dois meios a base de ovo, Stonebrink (ST) e Löwenstein-Jensen com piruvato de sódio (LJp), e dois meios a base de ágar, ágar sangue tuberculose (B83) e Middlebrook 7H11 (7H11). Cada meio foi incubado a 37°C por 90 dias, em três condições de incubação: em atmosfera normal, em atmosfera com acréscimo de 10% de dióxido de carbono (CO 2 ), e em atmosfera normal em tubos fechados com algodão hidrófobo queimado e subsequentemente fechado com rolha para criar uma atmosfera microaerófila. As colônias apareceram mais rapidamente e em maior número quando incubadas em atmosfera com 10% de CO 2 (p < 0,01), independente dos meios. As micobactérias cresceram em maior abundância e mais rapidamente no meio B83 aos 30 dias de i...

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Section: Discussionsupporting

confidence: 81%

Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Influence of the incubation conditions on culture media to optimize primary isolation of Mycobacterium bovis

Ikuta

Morato

Souza

et al. 2016

SCA

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“…The prevalence of FGTB in infertility clinics has been reported to range from 1 to 19% (2,11,13). In addition to M. tuberculosis infection, M. bovis infection has been reported to occur in FGTB (4, 7).The failure to isolate M. bovis in the present study may be due to use of inappropriate media (5,6). Nonspecific clinical presentation, inefficacy of laboratory diagnostic tests, and inaccessibility of reproductive clinics have resulted in underreporting of FGTB.…”

mentioning

confidence: 37%

Association of Tuberculous Endometritis with Infertility and Other Gynecological Complaints of Women in India

Kumar¹,

Shah²,

Singhal³

et al. 2008

J Clin Microbiol

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Endometrial biopsy samples derived from 393 patients with assorted gynecological complaints were investigated for mycobacterial infection. By employment of four different techniques, mycobacterial pathogens were detected irrespective of the nature/type of clinical complaint. Mycobacterium tuberculosis was the predominant pathogen detected among the samples investigated.Tuberculosis occurs worldwide and causes widespread morbidity and mortality. Pulmonary and extrapulmonary sites are known to be associated with Mycobacterium tuberculosis infection. In fact, it is well known that pulmonary tuberculosis patients go on to develop extrapulmonary tuberculosis. One such manifestation is the occurrence of female genital tuberculosis (FGTB). The spread of the pathogen to fallopian tubes, endometria, and ovaries, leading to a variety of clinical conditions, has been described previously (1,8,15). The present study was undertaken to detect mycobacterial infection in endometrial biopsy (EB) samples collected from patients registered in the gynecological outpatient department of the All India Institute of Medical Sciences, New Delhi, India.Three hundred ninety-three patients attending the obstetrics and gynecology outpatient department of the All India Institute of Medical Sciences were included in the study. Of these, 285 were infertility patients, 80 had menstrual dysfunction complaints, 17 had chronic lower abdominal or pelvic pain, and the remaining 11 were patients with complaints such as ovarian cyst, fibroid, prolapsed uterus, and postrecanalization. The EB samples were processed as described by Chakravorty et al. (3). Four methods were used to detect mycobacteria in the EB samples. The processed EB extracts were microscopically examined for acid-fast bacilli (AFB), processed for isolation of mycobacteria by inoculation on Lowenstein-Jensen medium, and processed for extraction of target DNA by nested PCR (N-PCR) (12). Culture results at the time of this writing were available for 262 samples. Two hundred ninety-five EB samples were processed for histopathological examination by hematoxylin and eosin staining. The N-PCR for the hupB DNA target was carried out as described previously (10). The N-PCR products were electrophoresed on 10% polyacrylamide gel and stained with ethidium bromide. The amplicon sizes for M. tuberculosis and Mycobacterium bovis were ϳ116 bp and 89 bp, respectively. Species-level identification of the isolates obtained was done by spoligotyping (9) and by standard biochemical tests (16). Randomly selected EB samples showing dual bands (116 and 89 bp) were cloned into the pGEMT vector by using a TA cloning kit (Promega). The clones were sequenced at the DNA sequencing facility, South Campus Delhi University, New Delhi, India.The detection and identification of M. tuberculosis and M. bovis in representative EB specimens are depicted in Fig. 1. N-PCR-amplified products equivalent to 116 bp were obtained for five of the seven samples (lanes 1 to 3, 7, and 8). These samples were considered to be infecte...

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Occurrence of tuberculosis among people exposed to cattle in Bangladesh

Sarkar

Haider

Islam

et al. 2023

Veterinary Medicine & Sci

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Comparison of media used for the primary isolation of Mycobacteriurm bovis by veterinary and medical diagnostic laboratories

Cited by 22 publications

References 6 publications

Influence of the incubation conditions on culture media to optimize primary isolation of Mycobacterium bovis

Influence of the incubation conditions on culture media to optimize primary isolation of Mycobacterium bovis

Association of Tuberculous Endometritis with Infertility and Other Gynecological Complaints of Women in India

Occurrence of tuberculosis among people exposed to cattle in Bangladesh

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