The NH 4 HCO 3 -DTPA (AB-DTPA), 1 MNH 4 HCO 3 , 0.005 M DTPA, pH=7.6, was proposed as a multi-element extractant, for evaluating macro and micronutrients availability to plants. AB-DTPA was also evaluated as a soil test, for assessing boron availability and toxicity to alfalfa. In a pot experiment, ten soils of Northern Greece were used to assess AB-DTPA as an extractant of available boron to wheat (Triticum aestivum L., cv. Yecora), in comparison with hot water and saturation extract. Boron (B) was added as borax (Na 2 B 4 O 7 *10H 2 O) to the ten soils, at rates equal to 0, 3, and 5 mg B kg -1 . Wheat was grown in pots containing the boron amended soils to the stage of tillering, and dry aboveground biomass, B concentration and B uptake by wheat were determined. AB-DTPA extractable B was significantly greater than saturation extract and similar to hot water at each B application rate, and was correlated significantly with hot water (r=0.84), or with saturation extract (r=0.48). Extractable boron by all extractants, boron concentration in wheat and boron uptake were significantly affected by the soil x B application rate interaction. In assessing B availability to wheat using AB-DTPA as a soil 669 670 MATSI, ANTONIADIS, AND BARBAYIANNIS test, CEC should be included in the regression equation for B concentration, or pH for B uptake. However, the corresponding adjusted coefficients of determination for B concentration (adjusted R 2 =0.46) and B uptake (adjusted R 2 =0.48) were similar or lower to those of hot water (adjusted R 2 =0.45 and 0.60, respectively) and the saturation extract (adjusted R 2 =0.70 and 0.49, respectively), when the latter two soil tests were used in the regression equations without the inclusion of any soil property.