The aim of this study was to analyze the differences in terms of (1) muscle activation patterns; (2) metabolic power (MP) and energy cost (EC) estimated via two determination methods (i.e., the Global Positioning System [GPS] and electromyography-based [EMG]); and (3) the apparent efficiency (AE) of 30-m linear sprints in seventeen elite U17 male soccer players performed under different conditions (i.e., unloaded sprint [US], parachute sprint [PS], and four incremental sled loads [SS15, SS30, SS45, SS60, corresponding to 15, 30, 45 and 60 kg of additional mass]). In a single testing session, each participant executed six trials (one attempt per sprint type). The results indicated that increasing the sled loads led to a linear increase in the relative contribution of the quadriceps (R2 = 0.98) and gluteus (R2 = 0.94) and a linear decrease in hamstring recruitment (R2 = 0.99). The MP during the US was significantly different from SS15, SS30, SS45, and SS60, as determined by the GPS and EMG approaches (p-values ranging from 0.01 to 0.001). Regarding EC, significant differences were found among the US and all sled conditions (i.e., SS15, SS30, SS45, and SS60) using the GPS and EMG methods (all p ≤ 0.001). Moreover, MP and EC determined via GPS were significantly lower in all sled conditions when compared to EMG (all p ≤ 0.001). The AE was significantly higher for the US when compared to the loaded sprinting conditions (all p ≤ 0.001). In conclusion, muscle activation patterns, MP and EC, and AE changed as a function of load in sled-resisted sprinting. Furthermore, GPS-derived MP and EC seemed to underestimate the actual neuromuscular and metabolic demands imposed on youth soccer players compared to EMG.