Comparison of Metal-Based Nanoparticles and Nanowires: Solubility, Reactivity, Bioavailability and Cellular Toxicity

Wall, Johanna; Şeleci, Didem Ağ; Schworm, Feranika; Neuberger, Ronja; Link, Martin; Hufnagel, Matthias; Schumacher, Petra B.; Schulz, Florian; Heinrich, Uwe; Wohlleben, Wendel; Hartwig, Andrea

doi:10.3390/nano12010147

Cited by 9 publications

(5 citation statements)

References 47 publications

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“…The minimum detectable size was 12 nm so that 97% of the CeO 2 NM212 particles detected by TEM could be determined by (LA)–spICP–MS. The size distributions obtained by (LA)–spICP–MS were slightly broader than those determined from the micrograph most likely resulting from slight CeO 2 NM212 aggregates/agglomerates, as present in aqueous media . The mean particle size and the size distribution obtained by LA–spICP–MS compared to pneumatic nebulizer-based spICP–MS were in good agreement, supporting the fact that the particles did not undergo fragmentation during the LA.…”

Section: Resultssupporting

confidence: 51%

“…The size distributions obtained by (LA)−spICP−MS were slightly broader than those determined from the micrograph most likely resulting from slight CeO 2 NM212 aggregates/agglomerates, as present in aqueous media. 38 The mean particle size and the size distribution obtained by LA−spICP−MS compared to pneumatic nebulizer-based spICP−MS were in good agreement, supporting the fact that the particles did not undergo fragmentation . For LA−spICP−MS analysis, the particle mass was determined using liquid calibration with dissolved metal standards, and the particle size was determined assuming a cubic shape.…”

Section: ■ Results and Discussionmentioning

confidence: 55%

“…36 Particle sizes range from 10 to 60 nm with a mean particle size of 26.4 ± 10.7 nm. Previously reported particle sizes of CeO 2 NM212 ranged from 28.4 ± 10.4 nm 37 and 21.5 ± 0.3 nm 38 (both Feret maximum diameter) to 15.6 ± 1 nm 36 (Feret minimum diameter), demonstrating some disparity in the size measurement of CeO 2 NPs, which needs to be considered when particle sizes obtained by TEM are compared to spICP−MS results. Since the TEM micrograph provides a two-dimensional projection of an electron-dense structure, the obtained Feret diameter depends on the particle orientation during the measurement.…”

Section: ■ Results and Discussionmentioning

confidence: 93%

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Calibration Strategy to Size and Localize Multi-Shaped Nanoparticles in Tissue Sections Using LA–spICP–MS

et al. 2023

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In the field of nanotoxicology, the detection and size characterization of nanoparticles (NPs) in biological tissues become increasingly important. To gain information on both particle size and particle distribution in histological sections, laser ablation and single particle inductively coupled plasma–mass spectrometry (LA–spICP–MS) was used in combination with a liquid calibration of dissolved metal standards via a pneumatic nebulizer. In the first step, the particle size distribution of Ag NPs embedded in matrix-matched gelatine standards introduced via LA was compared with that of Ag NPs in a suspension and nebulizer-based ICP–MS. The data show that the particles remained intact by the ablation process as confirmed by transmission electron microscopy. Moreover, the optimized method was applied to CeO2 NPs that are highly relevant for (eco-)toxicological research but, unlike Ag NPs, are multi-shaped and have a broad particle size distribution. Upon analyzing the particle size distribution of CeO2 NPs in cryosections of rat spleen, CeO2 NPs were found to remain unchanged in size over 3 h, 3 d, and 3 weeks post-intratracheal instillation, with the fraction of smaller particles reaching the spleen first. Overall, LA–spICP–MS combined with a calibration based on dissolved metal standards is a powerful tool to simultaneously localize and size NPs in histological sections in the absence of particle standards.

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Section: Resultssupporting

confidence: 51%

Section: ■ Results and Discussionmentioning

confidence: 55%

Section: ■ Results and Discussionmentioning

confidence: 93%

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Calibration Strategy to Size and Localize Multi-Shaped Nanoparticles in Tissue Sections Using LA–spICP–MS

et al. 2023

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“…In the present study, therefore, we aimed to obtain toxicity profiles for all three particles and to compare them with water-soluble Cr(VI) and Cr(III). To this end, we applied gene expression analyses using high-throughput RT-qPCR, established previously in our group using the BioMark HD system [11], and which has been used successfully for metal-based nanomaterials, both after submersed and air-liquid interface (ALI) exposure [16][17][18]. This method enables the parallel investigation of 96 samples with regard to their impact on 95 genes of interest.…”

Section: Gene Expression Analysismentioning

confidence: 99%

Impact of Nano- and Micro-Sized Chromium(III) Particles on Cytotoxicity and Gene Expression Profiles Related to Genomic Stability in Human Keratinocytes and Alveolar Epithelial Cells

et al. 2022

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Exposure to Cr(VI) compounds has been consistently associated with genotoxicity and carcinogenicity, whereas Cr(III) is far less toxic, due to its poor cellular uptake. However, contradictory results have been published in relation to particulate Cr2O3. The aim of the present study was to investigate whether Cr(III) particles exerted properties comparable to water soluble Cr(III) or to Cr(VI), including two nano-sized and one micro-sized particles. The morphology and size distribution were determined by TEM, while the oxidation state was analyzed by XPS. Chromium release was quantified via AAS, and colorimetrically differentiated between Cr(VI) and Cr(III). Furthermore, the toxicological fingerprints of the Cr2O3 particles were established using high-throughput RT-qPCR and then compared to water-soluble Cr(VI) and Cr(III) in A549 and HaCaT cells. Regarding the Cr2O3 particles, two out of three exerted only minor or no toxicity, and the gene expression profiles were comparable to Cr(III). However, one particle under investigation released considerable amounts of Cr(VI), and also resembled the toxicity profiles of Cr(VI); this was also evident in the altered gene expression related to DNA damage signaling, oxidative stress response, inflammation, and cell death pathways. Even though the highest toxicity was found in the case of the smallest particle, size did not appear to be the decisive parameter, but rather the purity of the Cr(III) particles with respect to Cr(VI) content.

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“…Although many studies on nanotoxicity are produced every year [23,24,[34][35][36][37][38], the mechanisms that trigger cytotoxicity are not yet fully elucidated. Moreover, the cellular effects of NPs are dependent on several factors, such as particle size, surface ligands, their agglomeration state, as well as the dose and exposure time in addition to the cell line being studied [3,22,26].…”

Section: Introductionmentioning

confidence: 99%

Titanium Dioxide Nanoparticle (TiO2 NP) Induces Toxic Effects on LA-9 Mouse Fibroblast Cell Line

Fattori

Brassolatti

Feitosa

et al. 2023

Cell Physiol Biochem

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Background/Aims: Titanium dioxide nanoparticles (TiO2 NPs) are extensively applied in the industry due to their photocatalytic potential, low cost, and considerably low toxicity. However, new unrelated physicochemical properties and the wide use of nanoparticles brought concern about their toxic effects. Thereby, we evaluated the cytotoxicity of a TiO2 NP composed of anatase and functionalized with sodium carboxylate ligands in a murine fibroblast cell line (LA-9). Methods: Scanning Electron Microscopy (SEM), Dynamic Light Scattering (DLS), and ATR-FTIR spectroscopy were applied to determine nanoparticle physicochemical properties. The cell viability (MTT assay) and clonogenic survival were analyzed in fibroblasts exposed to TiO2 NP (50, 150, and 250 µg/mL) after 24h. Moreover, oxidative stress, proinflammatory state, and apoptosis were evaluated after 24h. Results: TiO2 NP characterization showed an increased hydrodynamic size (3.57 to 7.62 nm) due to solvent composition and a heterogeneity dispersion in water and cell culture media. Also, we observed a zeta potential increased from -20 to -11 mV in function of protein adsorption. TiO2 NP reduced fibroblast cell viability and induced ROS production at the highest concentrations (150 and 250 µg/mL). Moreover, TiO2 NP reduced the fibroblasts clonogenic survival at the highest concentration (250 µg/mL) on the 7th day after the 24h exposure. Nevertheless, TiO2 NP did not affect the fibroblast proinflammatory cytokines (IL-6 and TNF) secretion at any condition. Early and late apoptotic fibroblast cells were detected only at 150 µg/mL TiO2 NP after 24h. Conclusion: Probably, TiO2 NP photocatalytic activity unbalanced ROS production which induced apoptosis and consequently reduced cell viability and metabolic activity at higher concentrations.

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Comparison of Metal-Based Nanoparticles and Nanowires: Solubility, Reactivity, Bioavailability and Cellular Toxicity

Cited by 9 publications

References 47 publications

Calibration Strategy to Size and Localize Multi-Shaped Nanoparticles in Tissue Sections Using LA–spICP–MS

Calibration Strategy to Size and Localize Multi-Shaped Nanoparticles in Tissue Sections Using LA–spICP–MS

Impact of Nano- and Micro-Sized Chromium(III) Particles on Cytotoxicity and Gene Expression Profiles Related to Genomic Stability in Human Keratinocytes and Alveolar Epithelial Cells

Titanium Dioxide Nanoparticle (TiO2 NP) Induces Toxic Effects on LA-9 Mouse Fibroblast Cell Line

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