Comparison of metal toxicity bioassays based on inhibition of sporulation and spore release inUlva pertusa

Han, Young Seok; Kumar, Anita Suresh; Han, Taejun

doi:10.1007/bf03216460

Cited by 11 publications

(9 citation statements)

References 9 publications

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“…Bioasai Mikrotoks sangat efektif menentukan toksisitas logam dalam sampel lingkungan, walau bagaimanapun metode Mikrotoks ini memiliki sensitivitas rendah, waktu respon yang lama (15 menit hingga Jam). Hsieh et al, (2004) Pengujian toksistas bahan beracun lainnya telah dilaporkan menggunakan bioasai berbasis organisme hidup seperti bakteri Escherichia coli dimodifikasi dengan pbenzoquinone untuk menentukan ketoksikan logam Ag(I) (Yu et al, 2013), remis Perna viridis untuk menilai ketoksikan logam Ag (I) (Vijayavel, 2010), ikan Capoeta fusca untuk memantau ketoksian logam Hg(I) dan Ag(I) (Mansouri et al, 2011), algae Ulva pertusa untuk menganalisis ketoksikan logam Ag(I), As(I), Cd(II), Co(II), Cr(VI) dan Cu(II) (Han et al, 2009), copepod laut Acartia tonsa untuk menilai ketokiksian Ag(I) (Pedrosa et al, 2007) dan Pseudokirchneriella subcapitata dan Chlamydomonas rein-hardtii untuk mengevaluasi ketoksikan Ag(I) (Hiriart-Baer et al, 2006). Bioasai toksisitas ini telah berhasil menentukan ketoksikan dalam sampel lingkungan, tetapi waktu inkubasi antara organisme dan bahan toksik Ag(I) memerlukan waktu respons yang lama 6-96 jam.…”

Section: Beberapaunclassified

“…Berdasarkan data pada Tabel 2, prestasi toksisitas bioasai bakteri laut A. fisheri yang dibandingkan dengan beberapa pengujian secara bioasai menggunakan berbagai organisme. Toksisitas bioasai menggunakan bakteri A. fischeri menunjukkan prestasi yang lebih baik dalam waktu respons jika dibandingkan dengan bioasai menggunakan bakteri (Yu et al, 2013), ikan (Mansouri et al, 2011), ganggan hijau (Han et al, 2009) dan kerang hijau (Vijayavel 2010). Nilai EC50% yang hampir sama yang diperoleh dalam penelitian ini dengan hasil yang dilaporkan menggunakan E. coli yang dimodifikasi dengan benzoquinone (Yu et al, 2013).…”

Section: Hasil Dan Pembahasanunclassified

“…Nilai EC50% yang hampir sama yang diperoleh dalam penelitian ini dengan hasil yang dilaporkan menggunakan E. coli yang dimodifikasi dengan benzoquinone (Yu et al, 2013). Walaubagimanapun, hasil penelitian ini memiliki nilai EC50% lebih tinggi dibandingkan dengan ikan (Mansouri et al, 2011) ganggan hijau (Han et al, 2009) dan kerang hijau (Vijayavel 2010).…”

Section: Hasil Dan Pembahasanunclassified

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Penentuan Cepat Toksisitas Logam Perak Menggunakan Bioluminesen Bakteri Laut Aliivibrio fischeri, Beijerinck, 1889 (Gammaproteobacteria: Vibrionaceae)

2020

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Rapid determination of toxicity based on changes in the bioluminescent signal of marine bacterium Aliivibrio fischeri (A. fischeri) to evaluate toxicity of Ag(I) has been successfully developed. Assessment of toxicity was designed using inhibition of bioluminescent signal from A. fischeri bacteria, which was exposed with toxic material of Ag(I). This metal ion was utilized as a model of toxic material to evaluate the effects of cytotoxicity on bacteria cell. Measurement of bioluminescent were taken based on differences in bacterial cell signals before and after exposure to Ag(I) ion at an emission wavelength of 488±2 nm. The concentration of bacterial cell was used to assess the toxicity of Ag(I) at optical density (OD600 nm) of 0.78 Abs. The results found that the linear response of Ag toxicity was in the range of 0.05–10 mg/L, with EC50% of 8.42 mg/L for 4 minutes. The repeatability value within the relative standard deviation (RSD) was 2.5-4.7% (n=8). The results demonstrated that the marine bacteria of A. fischeri have good potential to evaluate toxicity of toxic material in environmental samples. Penentuan cepat toksisitas berdasarkan perubahan sinyal bioluminesen bakteri laut Aliivibrio fischeri (A. fischeri) untuk mengevaluasi toksisitas logam perak (Ag(I)) telah sukses dikembangkan. Penilaian toksisitas didesain berdasarkan penghambatan sinyal bioluminesen bakteri A. fischeri oleh bahan toksik. Ion logam Ag(I) digunakan sebagai model bahan toksik untuk menilai efek sitotiksisitas pada sel bakteri. Pengukuran bioluminesen diambil berdasarkan perbedaan sinyal sel bakteri sebelum dan sesudah diekspos pada ion logam Ag(I) pada panjang gelombang emisi 488±2 nm. Konsentrasi sel bakteri yang digunakan untuk menilai toksisitas Ag(I) pada optikal densitas 600 (OD 600 nm) = 0.78 Abs. Hasil kajian ditemukan bahwa respons linear toksisitas Ag(I) pada rentang 0.05–10 mg/L, dengan nilai EC50% sebesar 8.42 mg/L pada waktu respons 4 menit dan nilai repeatibilitas toksisitas diperoleh sebesar 2.5-4.7 % RSD (relatif standar deviasi, n=8). Hasil ini menunjukkan bahwa bakteri laut A. fischeri memiliki potensi yang baik untuk menilai toksisitas bahan toksik dalam sampel lingkungan.

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Section: Beberapaunclassified

Section: Hasil Dan Pembahasanunclassified

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Penentuan Cepat Toksisitas Logam Perak Menggunakan Bioluminesen Bakteri Laut Aliivibrio fischeri, Beijerinck, 1889 (Gammaproteobacteria: Vibrionaceae)

2020

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“…The test measures the inhibition of the reproduction on the margins of the thallus by quantifying the colour changes exhibited by the thallus (i.e., caused by the release of reproductive cells) [ 20 ]. Ecotoxicity testing using U. australis has some advantages over conventional chemical techniques, namely, ease of use, sensitivity, cost, efficiency, environmental relevance, repeatability, and reproducibility [ 16 , 19 , 20 , 22 , 23 , 24 , 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Metal Toxicity across Different Thallus Sections of the Green Macroalga, Ulva australis

Lee

Kim

Depuydt

et al. 2023

Toxics

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We aimed to identify functional differences between different sections of the thallus of Ulva australis and develop tissue-endpoint combinations to assess the toxicity of six metals (i.e., Ag, As, Cd, Cr, Cu, and Ni). EC50 values for these metals in three sections of the thallus of Ulva were obtained for multiple endpoints: relative growth rate (RGR), chlorophyll a fluorescence, pigment contents, and the expression of the photosynthesis-related gene, rbcL. The responses of the endpoints varied across the respective thallus sections; overall, the most toxic metals were Ag and Cu. These endpoints were the best for evaluating metal toxicity: ETRmax of the middle thallus sections for Ag toxicity; RGR of the middle thallus section for As and Cd; ETRmax of the marginal thallus section for Cr; Chl b contents of the marginal thallus section for Cu; RGR of the basal thallus section for Ni. The EC50 values for the inhibition of ETRmax in middle (0.06 mg∙L−1) and Chl b in the marginal thallus sections (0.06 mg∙L−1) were all lower than those of the quality standard for wastewater discharge values of Ag and Cu in Republic of Korea and the US, pointing to the suitability of U. australis-based endpoints for risk assessment.

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“…Guidelines for TIEs recommend a range of test organisms, but no complete protocols have been developed with macroalgae, despite their significance as bio-engineers and primary producers in coastal and estuarine waters. A macroalgal toxicity test, based on the inhibition of sporulation in the macroalga, Ulva pertusa, has recently been proposed as a new ISO standard method which has several advantages over other currently employed techniques (Han and Choi, 2005;Han et al, 2007Han et al, , 2008Han et al, , 2009). For example, this cost and time-effective test only requires a cell plate and a small volume of water.…”

Section: Introductionmentioning

confidence: 99%

Application of the Ulva pertusa bioassay for a toxicity identification evaluation and reduction of effluent from a wastewater treatment plant

Kim

Han²,

Kim

et al. 2015

Front. Environ. Sci.

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A toxicity identification evaluation (TIE) based on Ulva pertusa spore release was conducted in 3 phases for the identification of the major toxicants in effluent from a wastewater treatment plant (WTP) and the receiving water in an adjacent stream. The toxicity of the final effluent (FE), as compared with raw wastewater, and primary and secondary effluent, showed a greater change over 12-monthly sampling events and appeared to have impacts on the toxicity of the downstream water with a significant correlation (r 2 = 0.89, p < 0.01). In Phase I, toxicity characterization indicated that cations were likely to be the responsible toxicants for the FE. In Phase II, cations such as Cu, Ni, and Zn were found in the FE at higher concentrations than the EC 50 concentrations determined for the standard corresponding metals. When the concentrations of each metal in the FE samples were plotted against the respective toxicity units, only zinc showed a statistically significant correlation with toxicity (r 2 = 0.86, p < 0.01). In Phase III, using spiking and mass balance approaches, it was confirmed that Zn was the major toxicant in the effluent from the WTP. Following a change in the Fenton reagent used, to one with a lower Zn content, the toxicity of the FE greatly decreased in subsequent months. The TIE developed here enabled the toxicity of FEs of the WTP to be tracked and for Zn, originating from a reagent used for Fenton treatment, to be successfully identified as the key toxicant. The TIE method based on U. pertusa demonstrated utility as a low cost and simple tool to identify the risk factors for industrial effluents and provided information on regulatory control and management.

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Comparison of metal toxicity bioassays based on inhibition of sporulation and spore release inUlva pertusa

Cited by 11 publications

References 9 publications

Penentuan Cepat Toksisitas Logam Perak Menggunakan Bioluminesen Bakteri Laut Aliivibrio fischeri, Beijerinck, 1889 (Gammaproteobacteria: Vibrionaceae)

Penentuan Cepat Toksisitas Logam Perak Menggunakan Bioluminesen Bakteri Laut Aliivibrio fischeri, Beijerinck, 1889 (Gammaproteobacteria: Vibrionaceae)

Metal Toxicity across Different Thallus Sections of the Green Macroalga, Ulva australis

Application of the Ulva pertusa bioassay for a toxicity identification evaluation and reduction of effluent from a wastewater treatment plant

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