Comparison of methods for evaluating radiolabelled Annexin A5 uptake in pre-clinical PET oncological studies

Grafström, Jonas; Stone‐Elander, Sharon

doi:10.1016/j.nucmedbio.2014.07.003

Cited by 5 publications

(4 citation statements)

References 44 publications

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“…Our findings support the reliable use of both graphical analysis methods in addition to the standard 1TC model for tracer kinetic analysis of V T . These findings agree with previous studies using other PET tracers that compared various graphical models, including the Logan method, finding the results to be in agreement with standard compartment models, but computationally simpler, and potentially more robust [ 24 – 27 ].…”

Section: Discussionsupporting

confidence: 91%

Reproducible quantification of cardiac sympathetic innervation using graphical modeling of carbon-11-meta-hydroxyephedrine kinetics with dynamic PET-CT imaging

Wang

Miner

et al. 2018

EJNMMI Res

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BackgroundGraphical methods of radiotracer kinetic modeling in PET are ideal for parametric imaging and data quality assurance but can suffer from noise bias. This study compared the Logan and Multilinear Analysis-1 (MA1) graphical models to the standard one-tissue-compartment (1TC) model, including correction for partial-volume effects, in dynamic PET-CT studies of myocardial sympathetic innervation in the left ventricle (LV) using [11C]HED.MethodsTest and retest [11C]HED PET imaging (47 ± 22 days apart) was performed in 18 subjects with heart failure symptoms. Myocardial tissue volume of distribution (VT) was estimated using Logan and MA1 graphical methods and compared to the 1TC standard model values using intraclass correlation (ICC) and Bland-Altman analysis of the non-parametric reproducibility coefficient (NPC).ResultsA modeling start-time of t* = 5 min gave the best fit for both Logan and MA1 (R2 = 0.95) methods. Logan slightly underestimated VT relative to 1TC (p = 0.002), whereas MA1 did not (p = 0.96). Both the MA1 and Logan models exhibited good-to-excellent agreement with the 1TC (MA1-1TC ICC = 0.96; Logan-1TC ICC = 0.93) with no significant differences in NPC between the two comparisons (p = 0.92). All methods exhibited good-to-excellent test-retest repeatability with no significant differences in NPC (p = 0.57).ConclusionsLogan and MA1 models exhibited similar agreement and variability compared to the 1TC for modeling of [11C]HED kinetics. Using t* = 5 min and partial-volume correction produced accurate estimates of VT as an index of myocardial sympathetic innervation.Electronic supplementary materialThe online version of this article (10.1186/s13550-018-0421-5) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 91%

Reproducible quantification of cardiac sympathetic innervation using graphical modeling of carbon-11-meta-hydroxyephedrine kinetics with dynamic PET-CT imaging

Wang

Miner

et al. 2018

EJNMMI Res

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“…5), with a modest 40% increase in radioactivity accumulation following drug treatment detectable by average counts in excised tumors. Non-specific tumor retention of Annexin V in the vascular space has recently been attributed to changes in enhanced permeability and retention (EPR) effects at baseline and post therapy (39). Despite advances in both site-specific and PET labeling strategies little has been achieved to improve the in vivo profile and tumor binding of this radiotracer (40).…”

Section: Discussionmentioning

confidence: 99%

A Systematic Comparison of 18F-C-SNAT to Established Radiotracer Imaging Agents for the Detection of Tumor Response to Treatment

Witney

Hoehne

Reeves

et al. 2015

Clinical Cancer Research

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Purpose An early readout of tumor response to therapy through measurement of drug or radiation-induced cell death may provide important prognostic indications and improved patient management. It has been shown that the uptake of 18F-C-SNAT can be used to detect early response to therapy in tumors by positron emission tomography via a mechanism of caspase 3-triggered nanoaggregation. Experimental Design Here, we compared the preclinical utility of 18F-C-SNAT for the detection of drug-induced cell death to clinically evaluated radiotracers, 18F-FDG, 99mTc-Annexin V and 18F-ML-10 in tumor cells in culture, and in tumor-bearing mice in vivo. Results In drug-treated lymphoma cells, 18F-FDG, 99mTc-Annexin V and 18F-C-SNAT cell-associated radioactivity correlated well to levels of cell death (R2 > 0.8; P < 0.001), with no correlation measured for 18F-ML-10 (R2 = 0.05; P > 0.05). A similar pattern of response was observed in two human NSCLC cell lines following carboplatin treatment. EL-4 tumor uptake of 99mTc-Annexin V and 18F-C-SNAT were increased 1.4- and 2.1-fold, respectively in drug-treated versus naïve control animals (P < 0.05), although 99mTc-Annexin V binding did not correlate to ex vivo TUNEL staining of tissue sections. A differential response was not observed with either 18F-FDG or 18F-ML-10. Conclusions We have demonstrated here that 18F-C-SNAT can sensitively detect drug-induced cell death in murine lymphoma and human NSCLC. Despite favorable image contrast obtained with 18F-C-SNAT, the development of next generation derivatives, using the same novel and promising uptake mechanism, but displaying improved biodistribution profiles, are warranted for maximum clinical utility.

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“…6d – f ) by subtracting the TACs of the non-targeting ligand from that of the targeting to give a measure of the specific uptake. Compartmental modeling can be used to further quantify the binding and non-specific uptakes (e.g., [ 29 , 30 ] reviewed in [ 7 ]). However, TACs from several of these studies (e.g., Figs.…”

Section: Discussionmentioning

confidence: 99%

Preclinical PET imaging of EGFR levels: pairing a targeting with a non-targeting Sel-tagged Affibody-based tracer to estimate the specific uptake

Cheng

Wållberg²,

Grafström

et al. 2016

EJNMMI Res

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Background: Though overexpression of epidermal growth factor receptor (EGFR) in several forms of cancer is considered to be an important prognostic biomarker related to poor prognosis, clear correlations between biomarker assays and patient management have been difficult to establish. Here, we utilize a targeting directly followed by a non-targeting tracer-based positron emission tomography (PET) method to examine some of the aspects of determining specific EGFR binding in tumors.

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Comparison of methods for evaluating radiolabelled Annexin A5 uptake in pre-clinical PET oncological studies

Abstract: These results provide additional support for the recognition that more detailed investigations on the effects of the tumour microenvironment on the targeting capability of imaging radiopharmaceuticals are needed.

Cited by 5 publications

References 44 publications

Reproducible quantification of cardiac sympathetic innervation using graphical modeling of carbon-11-meta-hydroxyephedrine kinetics with dynamic PET-CT imaging

Reproducible quantification of cardiac sympathetic innervation using graphical modeling of carbon-11-meta-hydroxyephedrine kinetics with dynamic PET-CT imaging

A Systematic Comparison of 18F-C-SNAT to Established Radiotracer Imaging Agents for the Detection of Tumor Response to Treatment

Preclinical PET imaging of EGFR levels: pairing a targeting with a non-targeting Sel-tagged Affibody-based tracer to estimate the specific uptake

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