Comparison of methods for separating fluorescently labelled placental extracellular vesicles from free stain

Dietary Lactobacillus rhamnosus GG extracellular vesicles enhance antiprogrammed cell death 1 (anti-PD-1) immunotherapy efficacy against colorectal cancer

“…LGG-EV were labeled with fluorescent dye Cy7, incubated at 37 °C for 2 h, and then centrifuged at 4 °C 150 000 g for 1.5 h to remove free dye as previously described. 21 EV sedimentation was further resuspended in PBS, and mice were administered Cy7-labeled EV by gavage (5 mg kg −1 ). The mice were imaged at 12 h using an IVIS Lamina XR-III (Caliper Life Sciences).…”

Extracellular Vesicles Derived From Stem Cells in Intervertebral Disc Degeneration

“…SEC has been utilized for the separation of EVs from a large variety of origins from both prokaryotes and eukaryotes, involving cells, plasma and serum, urine, synovial fluid, cerebrospinal fluids, and others ( Foers et al, 2018 ; Guan et al, 2020 ; Povero et al, 2020 ). Several studies have also shown the superiority of SEC, allowing purer EV preparations with easy implementation ( Allelein et al, 2021 ; Tsai et al, 2021 ). For instance, when EVs were separated from plasma via the UC technique, high-density lipoproteins (HDLs) were co-separated, because HDLs share a similar density range with EVs.…”