Comparison of microbiota and allergen profile in house dust from homes of allergic and non-allergic subjects- results from the GUSTO study

Chew, Lamony Jian Ming; Zulkifli, A; Ta, Le Duc Huy; Kuo, I-Chun; Goh, Angela; Teoh, Oon Hoe; Bever, Hugo Van; Gluckman, Peter D.; Yap, Fabian; Tan, Kok Hian; Chong, Yap Seng; Lee, Bee Wah

doi:10.1186/s40413-018-0212-5

Cited by 20 publications

(19 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In homes, studies were mostly done on floors, kitchen sinks, and bathrooms [5,11]. However, very little is known about microbial communities present in beds with which humans have extended daily exposure [12].…”

Section: Introductionmentioning

confidence: 99%

Environmental shaping of the bacterial and fungal community in infant bed dust and correlations with the airway microbiota

et al. 2020

View full text Add to dashboard Cite

Background: From early life, children are exposed to a multitude of environmental exposures, which may be of crucial importance for healthy development. Here, the environmental microbiota may be of particular interest as it represents the interface between environmental factors and the child. As infants in modern societies spend a considerable amount of time indoors, we hypothesize that the indoor bed dust microbiota might be an important factor for the child and for the early colonization of the airway microbiome. To explore this hypothesis, we analyzed the influence of environmental exposures on 577 dust samples from the beds of infants together with 542 airway samples from the Copenhagen Prospective Studies on Asthma in Childhood 2010 cohort. Results: Both bacterial and fungal community was profiled from the bed dust. Bacterial and fungal diversity in the bed dust was positively correlated with each other. Bacterial bed dust microbiota was influenced by multiple environmental factors, such as type of home (house or apartment), living environment (rural or urban), sex of siblings, and presence of pets (cat and/or dog), whereas fungal bed dust microbiota was majorly influenced by the type of home (house or apartment) and sampling season. We further observed minor correlation between bed dust and airway microbiota compositions among infants. We also analyzed the transfer of microbiota from bed dust to the airway, but we did not find evidence of transfer of individual taxa. Conclusions: Current study explores the influence of environmental factors on bed dust microbiota (both bacterial and fungal) and its correlation with airway microbiota (bacterial) in early life using high-throughput sequencing. Our findings demonstrate that bed dust microbiota is influenced by multiple environmental exposures and could represent an interface between environment and child.

show abstract

Section: Introductionmentioning

confidence: 99%

Environmental shaping of the bacterial and fungal community in infant bed dust and correlations with the airway microbiota

et al. 2020

View full text Add to dashboard Cite

show abstract

“…One limitation is that this study is cross-sectional, and the results cannot determine causality. To Chinese migrants, the change to a western environment is the combination of a different diet, less air pollution, exposure to new allergens and, greater hygiene, all of which can lead to different microbiota composition/diversity, and contribute to the increased allergies in AC children 33, 34. However, in this study we could only focus on the overall influence of a western environment on the microbiome and the relation with allergy.…”

Section: Discussionmentioning

confidence: 99%

Western oropharyngeal and gut microbial profiles are associated with allergic conditions in Chinese immigrant children

Guo

Ariff

et al. 2019

World Allergy Organization Journal

View full text Add to dashboard Cite

Background The allergy epidemic resulting from western environment/lifestyles is potentially due to modifications of the human microbiome. Therefore, it is of interest to study immigrants living in a western environment as well as their counterparts in the country of origin to understand differences in their microbiomes and health status. Methods We investigated 58 Australian Chinese (AC) children from Perth, Western Australia as well as 63 Chinese-born Chinese (CC) children from a city in China. Oropharyngeal (OP) and fecal samples were collected. To assess the microbiomes, 16s ribosomal RNA (rRNA) sequencing for variable regions V3 and V4 was used. Skin prick tests (SPT) were performed to measure the children's atopic status. Information on food allergy and wheezing were acquired from a questionnaire. Results AC children had more allergic conditions than CC children. The alpha diversity (mean species diversity) of both OP and gut microbiome was lower in AC children compared to CC children for richness estimate (Chao1), while diversity evenness (Shannon index) was higher. The beta diversity (community similarity) displayed a distinct separation of the OP and gut microbiota between AC and CC children. An apparent difference in microbial abundance was observed for many bacteria. In AC children, we sought to establish consistent trends in bacterial relative abundance that are either higher or lower in AC versus CC children and higher or lower in children with allergy versus those without allergy. The majority of OP taxa showed a consistent trend while the majority of fecal taxa showed a contrasting trend. Conclusion Distinct differences in microbiome compositions were found in both oropharyngeal and fecal samples of AC and CC children. The association of the OP microbiome with allergic condition is different from that of the gut microbiome in AC children. The microbiome profiles are changed by the western environment/lifestyle and are associated with allergies in Chinese immigrant children in Australia.

show abstract

“…36 Similarly, based on the findings from the Growing Up in Singapore Towards healthy Outcomes (GUSTO) birth cohort study, our group has reported that there were differential microbiota and allergen profiles in house dust collected from homes of allergic participants compared to healthy controls. 38 Houses of allergic participants had higher levels of Bacteroidaceae, Anaplasmataceae, and Leptospiraceae, all of which are gram negative. 38 Endotoxins which are associated with gram negative bacteria have also been shown to potentially increase risk of wheezing in children from a Boston study.…”

Section: Environmental Dust and Ncdsmentioning

confidence: 96%

“…38 Houses of allergic participants had higher levels of Bacteroidaceae, Anaplasmataceae, and Leptospiraceae, all of which are gram negative. 38 Endotoxins which are associated with gram negative bacteria have also been shown to potentially increase risk of wheezing in children from a Boston study. 39 Besides this, abundance of Bacteroidaceae was higher in gut microbiota of allergic Japanese subjects at 1 and 2 months of age, as compared to the non-allergic subjects which also suggests a possible link between environmental and gut microbiota.…”

Section: Environmental Dust and Ncdsmentioning

confidence: 96%

Developmental Origins of Health and Disease: Impact of environmental dust exposure in modulating microbiome and its association with non-communicable diseases

Ooi

Tan

Tay

et al. 2020

J Dev Orig Health Dis

Self Cite

View full text Add to dashboard Cite

Non-communicable diseases (NCDs) including obesity, diabetes, and allergy are chronic, multi-factorial conditions that are affected by both genetic and environmental factors. Over the last decade, the microbiome has emerged as a possible contributor to the pathogenesis of NCDs. Microbiome profiles were altered in patients with NCDs, and shift in microbial communities was associated with improvement in these health conditions. Since the genetic component of these diseases cannot be altered, the ability to manipulate the microbiome holds great promise for design of novel therapies in the prevention and treatment of NCDs. Together, the Developmental Origins of Health and Disease concept and the microbial hypothesis propose that early life exposure to environmental stimuli will alter the development and composition of the human microbiome, resulting in health consequences. Recent studies indicated that the environment we are exposed to in early life is instrumental in shaping robust immune development, possibly through modulation of the human microbiome (skin, airway, and gut). Despite much research into human microbiome, the origin of their constituent microbiota remains unclear. Dust (also known as particulate matter) is a key determinant of poor air quality in the modern urban environment. It is ubiquitous and serves as a major source and reservoir of microbial communities that modulates the human microbiome, contributing to health and disease. There are evidence that reported significant associations between environmental dust and NCDs. In this review, we will focus on the impact of dust exposure in shaping the human microbiome and its possible contribution to the development of NCDs.

show abstract

Comparison of microbiota and allergen profile in house dust from homes of allergic and non-allergic subjects- results from the GUSTO study

Cited by 20 publications

References 47 publications

Environmental shaping of the bacterial and fungal community in infant bed dust and correlations with the airway microbiota

Environmental shaping of the bacterial and fungal community in infant bed dust and correlations with the airway microbiota

Western oropharyngeal and gut microbial profiles are associated with allergic conditions in Chinese immigrant children

Developmental Origins of Health and Disease: Impact of environmental dust exposure in modulating microbiome and its association with non-communicable diseases

Contact Info

Product

Resources

About