Comparison of miRNA expression profiles in individuals with chronic or aggressive periodontitis

Amaral, Sérgio Antonucci; Pereira, Thais S.; Brito, João Artur Ricieri; Cortelli, Sheila Cavalca; Cortelli, José Roberto; Gomez, Ricardo Santiago; Costa, Fernando Oliveira; Cota, Luís Otávio Miranda

doi:10.1111/odi.12994

Cited by 28 publications

(22 citation statements)

References 51 publications

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“…Luan et al found that miRNAs upregulated due to periodontal disease in both humans and mice included miRNA-15a, miRNA-29b, miRNA-125a, miRNA-146a, miRNA-148/148a and miRNA-223, while miRNA-92 was downregulated [ 10 ]. It has also been found that there are no major qualitative differences in miRNA expression in chronic or aggressive periodontitis [ 48 ]. Different microarray studies have demonstrated the over-regulation of miRNA-223 in gingival tissue biopsies from patients with periodontitis compared to healthy gingiva [ 15 , 28 , 39 ].…”

Section: Mirna Molecular Signalling and Periodontal Diseasementioning

confidence: 99%

The Emerging Role of microRNA in Periodontitis: Pathophysiology, Clinical Potential and Future Molecular Perspectives

Santonocito

Polizzi

Palazzo

et al. 2021

IJMS

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During the last few decades, it has been established that messenger ribonucleic acid (mRNA) transcription does not inevitably lead to protein translation, but there are numerous processes involved in post-transcriptional regulation, which is a continuously developing field of research. MicroRNAs (miRNAs) are a group of small non-coding RNAs, which negatively regulate protein expression and are implicated in several physiological and pathological mechanisms. Aberrant expression of miRNAs triggers dysregulation of multiple cellular processes involved in innate and adaptive immune responses. For many years, it was thought that miRNAs acted only within the cell in which they were synthesised, but, recently, they have been found outside cells bound to lipids and proteins, or enclosed in extracellular vesicles, namely exosomes. They can circulate throughout the body, transferring information between cells and altering gene expression in the recipient cells, as they can fuse with and be internalised by the recipient cells. Numerous studies on miRNAs have been conducted in order to identify possible biomarkers that can be used in the diagnosis of periodontal disease. However, as therapeutic agents, single miRNAs can target several genes and influence multiple regulatory networks. The aim of this review was to examine the molecular role of miRNAs and exosomes in the pathophysiology of periodontal disease and to evaluate possible clinical and future implications for a personalised therapeutical approach.

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Section: Mirna Molecular Signalling and Periodontal Diseasementioning

confidence: 99%

The Emerging Role of microRNA in Periodontitis: Pathophysiology, Clinical Potential and Future Molecular Perspectives

Santonocito

Polizzi

Palazzo

et al. 2021

IJMS

View full text Add to dashboard Cite

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“…Although genetics play a more important role in AP than CP [ 30 ], only a few studies have demonstrated associations between miRNA expression and AP, mostly using gingival biopsies. Amaral et al [ 31 ] compared the expression patterns of 754 miRNAs between CP and AP, finding that hsa-miR-1274b, hsa-let-7b-5p, hsa-miR-24-3p, hsa-miR-19b-3p, hsa-miR-720, hsa-miR-126-3p, hsa-miR-17-3p, and hsa-miR-21-3p were upregulated in both groups, suggesting that there were no significant differences in miRNA expression patterns between CP and AP. Ghotloo et al [ 32 ] found an association between hsa-miR-146a and generalized aggressive periodontitis (GAP) and suggested that hsa-miR-146a may serve as an indicator of disease activity.…”

Section: Discussionmentioning

confidence: 99%

“…While there are more genetic risk factors for AP than for CP, few studies have evaluated miRNA expression profiles in AP. Amaral et al compared the expression patterns of miRNAs between CP and AP using gingival biopsies and PCR arrays [ 31 ], and Ghotloo et al [ 32 ] compared expression levels of hsa-miR-146a between AP patients and healthy controls using gingival biopsies and real-time PCR. To our best knowledge, no study has utilized salivary miRNAs to identify AP.…”

Section: Introductionmentioning

confidence: 99%

Differential expression of microRNAs in the saliva of patients with aggressive periodontitis: a pilot study of potential biomarkers for aggressive periodontitis

Lee

Kim

et al. 2020

J Periodontal Implant Sci

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Purpose The aim of this study was to compare microRNA (miRNA) gene expression in saliva using miRNA polymerase chain reaction (PCR) arrays in healthy and aggressive periodontitis (AP) patients. Methods PCR arrays of 84 miRNAs related to the human inflammatory response and autoimmunity from the saliva samples of 4 patients with AP and 4 healthy controls were performed. The functions and diseases related to the miRNAs were obtained using TAM 2.0. Experimentally validated targets of differentially expressed miRNAs were obtained from mirTarBase. Gene ontology terms and pathways were analyzed using ConsensusPathDB. Results Four downregulated miRNAs (hsa-let-7a-5p, hsa-let-7f-5p, hsa-miR-181b-5p, and hsa-miR-23b-3p) were identified in patients with AP. These miRNAs are associated with cell death and innate immunity, and they target genes associated with osteoclast development and function. Conclusions This study is the first analysis of miRNAs in the saliva of patients with AP. Identifying discriminatory human salivary miRNA biomarkers reflective of periodontal disease in a non-invasive screening assay is crucial for the development of salivary diagnostics. These data provide a first step towards the discovery of key salivary miRNA biomarkers for AP.

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“…MiR‐21 has been reported to be a key factor in various infectious diseases through a variety of regulatory effects on inflammation, osteogenesis, and osteoclastogenesis 40–44 . Overexpression of miR‐21 was observed in gingival tissue, peripheral blood, and periodontal ligament stem cells from periodontitis patients, as well as in gingival tissue in a mouse model of periodontitis 21,26,45–47 . P.g LPS could induce miR‐21 upregulation, which suggested a close association between miR‐21 and periodontal pathogen‐induced inflammation 21,26 .…”

Section: Periodontitis and Mirnasmentioning

confidence: 96%

microRNAs in inflammatory alveolar bone defect: A review

Yuan

Zhang

Huang

2020

J of Periodontal Research

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Inflammatory alveolar bone defects are caused by periodontal pathogens, are one of the most common oral diseases in the clinic, and are characterized by periodontal support tissue damage. MicroRNAs (miRNAs) can participate in a variety of inflammatory lesions and modulate bone metabolism through the posttranscriptional regulation of target genes. In recent years, studies have confirmed that some miRNAs play significant roles in the development of inflammatory alveolar bone defects. Therefore, we reviewed the correlation between miRNAs and inflammatory alveolar bone defects and elucidated the underlying mechanisms to provide new ideas for the prevention and treatment of inflammatory alveolar bone defects.

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Comparison of miRNA expression profiles in individuals with chronic or aggressive periodontitis

Cited by 28 publications

References 51 publications

The Emerging Role of microRNA in Periodontitis: Pathophysiology, Clinical Potential and Future Molecular Perspectives

The Emerging Role of microRNA in Periodontitis: Pathophysiology, Clinical Potential and Future Molecular Perspectives

Differential expression of microRNAs in the saliva of patients with aggressive periodontitis: a pilot study of potential biomarkers for aggressive periodontitis

microRNAs in inflammatory alveolar bone defect: A review

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