Comparison of mixed antiglobulin reaction and direct immunobead test for detection of sperm-bound antibodies in subfertile males

Rajah, Shantil V.; Parslow, J. M.; Howell, R. J. S.; Hendry, W. F.

doi:10.1016/0020-7292(93)90882-w

Cited by 13 publications

(10 citation statements)

References 17 publications

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“…Rajah et al [ 24] compared the sMAR and IBT tests for detecting surface‐bound antibodies on spermatozoa from subfertile men. The sMAR (IgG) is a cheap and quick screening test, but IBT (IgA) provides useful additional information on the class of antibody and that may be clinically more important.…”

Section: Discussionmentioning

confidence: 99%

A comparison of the sperm mixed‐agglutination reaction test with the peroxidase‐labelled protein A test for detecting antisperm antibodies in infertile men with varicocele

Isitmangil¹,

Yildirim

Orhan

et al. 1999

BJU International

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Objective To compare the sperm mixed‐agglutination reaction (sMAR) with the peroxidase‐labelled protein A method (POPA) in infertile patients with varicocele. Patients, subjects and methods The study comprised 30 men with a history of varicocele‐associated infertility and 30 fertile men (control group). Antisperm antibodies against spermatozoa in the semen and against progenitor spermatozoa in testicular tissue were detected using the two methods. Results The tests were positive in 15 (50%) of patients with both the sMAR and the POPA methods, while no autoantibodies were detected in the control group. There were no significant differences between the methods. The sensitivity and specificity of both tests was ≈93%, with no significant difference between them (P>0.05). Conclusion Both methods may be used for detecting sperm autoantibodies in infertile patients with varicocele.

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Section: Discussionmentioning

confidence: 99%

A comparison of the sperm mixed‐agglutination reaction test with the peroxidase‐labelled protein A test for detecting antisperm antibodies in infertile men with varicocele

Isitmangil¹,

Yildirim

Orhan

et al. 1999

BJU International

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“…The MAR test is an inexpensive, quick, and sensitive screening test in which sheep erythrocytes or latex particles coated with human IgG or IgA are used to detect and localize antibody-bound sperm [12,13]. The formation of mixed agglutinates between the particles/red blood cells and motile spermatozoa indicates the presence of IgG or IgA antibodies on the spermatozoa.…”

Section: Mixed Agglutination Reaction Testmentioning

confidence: 99%

Role and Significance of Sperm Function in Men with Unexplained Infertility

Esteves¹,

Verza²,

Sharma

et al. 2015

Unexplained Infertility

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“…IBT is more time consuming but it identifies the proportion of antibodybound sperm in a given sample, the antibody class and the location of antibodies on sperm surface. On the other hand, direct MAR test is an inexpensive, quick and sensitive screening test where sheep erythrocytes are used instead of immunobeads to detect and localize antibody-bound sperm [25,26]. Frequently, antibody-coated sperm may appear as a poor postcoital test [27].…”

Section: B)mentioning

confidence: 99%

The Role of Contemporary Andrology in Unraveling the Mystery of Unexplained Male Infertility

Hamada¹,

Esteves²,

Agarwal

2011

TORSJ

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Unexplained male infertility is a condition in which infertile men have normal semen analyses on multiple occasions with no obvious physical or endocrine abnormality when female partner infertility has been ruled out. In addition to erectile problems and coital factors, immune causes and dysfunctional sperm may contribute to such condition. Contemporary andrology may help analyze the unexplained male fertility problem on the basis of cellular and sub-cellular mechanisms and select the proper management. This review highlights the concept of unexplained male infertility and discusses the potential causes and its proper management in the era of modern andrology and assisted reproductive techniques.

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Comparison of mixed antiglobulin reaction and direct immunobead test for detection of sperm-bound antibodies in subfertile males

Cited by 13 publications

References 17 publications

A comparison of the sperm mixed‐agglutination reaction test with the peroxidase‐labelled protein A test for detecting antisperm antibodies in infertile men with varicocele

A comparison of the sperm mixed‐agglutination reaction test with the peroxidase‐labelled protein A test for detecting antisperm antibodies in infertile men with varicocele

Role and Significance of Sperm Function in Men with Unexplained Infertility

The Role of Contemporary Andrology in Unraveling the Mystery of Unexplained Male Infertility

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