Comparison of modified blotter and agar incubation methods for detecting fungi in Zinnia elegans seeds

Szopin´ska, D.; Tylkowska, K.; Deng, Cheng; Gao, Yangyang

doi:10.15258/sst.2012.40.1.04

Cited by 13 publications

(9 citation statements)

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“…and R. nigricans. This phenomenon was also observed by Szopińska et al (2012) in relation to zinnia seeds. The authors reported that an addition of PEG to the substrate favoured the growth of Cladosporium spp., whereas an addition of mannitol promoted the growth of Alternaria spp., B. cinerea, Cladosporium spp.…”

Section: Resultssupporting

confidence: 72%

Comparison of methods for detecting fungi in Silybum marianum (L.) Gaertn. seeds

Rosińska¹,

Jarosz²,

Szopińska³

et al. 2013

Folia Horticulturae

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Milk thistle (Silybum marianum (L.) Gaertn.) is one of the most important medicinal plants. The fungi infesting its seeds may negatively influence their germination and health-promoting properties. However, there is no standard method for S. marianum seed health testing. The aim of this study was to find a suitable method for the detection and identification of fungi in/on milk thistle seeds. The following tests were used: deep-freeze blotter test, blotter test with an addition of mannitol, blotter test with an addition of polyethylene glycol, agar test on potato-dextrose agar (PDA), agar test on PDA after seed disinfection, agar test on reduced PDA and an agar test on reduced PDA after seed disinfection. Seeds were incubated for 10 and 14 days. The most prevalent fungi were: Alternaria alternata, Botrytis cinerea, Cladosporium spp., Fusarium spp., Penicillium spp., Rhizopus nigricans and Verticillium spp. A deep-freeze blotter test followed by a blotter test with an addition of mannitol and a blotter test with an addition of polyethylene glycol performed for 14 days could be recommended for the further study of milk thistle seed health testing, as they favoured the growth of the most important fungi.

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Section: Resultssupporting

confidence: 72%

Comparison of methods for detecting fungi in Silybum marianum (L.) Gaertn. seeds

Rosińska¹,

Jarosz²,

Szopińska³

et al. 2013

Folia Horticulturae

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“…From the above, it can be clearly seen that A. alternata is a fungus that can infect many fruits, however, although it is not within the scope of this chapter, this fungus has been reported to infect other parts of plants like seeds (Szopinska et al, 2012), leaves (Harteveld et al, 2013), stems (Choi et al, 2010) and flowers (Espinoza-Verduzco et al, 2012) reducing the agricultural production either directly by infecting the fruit or indirectly by impairing the physiology of plant photosynthesis (Lagopodi and Thanassoulopoulos, 1998;Qiang et al, 2010).…”

mentioning

confidence: 94%

Alternaria alternata (Black Rot, Black Spot)

Troncoso‐Rojas

Tiznado‐Hernández

2014

Postharvest Decay

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“…The high effectiveness of the blotter test with an addition of mannitol in detection of Alternaria spp. on zinnia seeds was reported by Szopińska et al [40].…”

Section: Discussionmentioning

confidence: 64%

Incubation methods for the detection of fungi associated with caraway (Carum carvi L.) seeds

2015

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S u m m a r y Introduction: Infestation with fungi may significantly affect the quality of seeds. However, there is no standard method for caraway (Carum carvi L.) seed health testing. Objective:The aim of the present study was to determine the most efficient method of the detection of fungi associatyed with caraway seeds. Methods: Seven incubation methods for evaluation of health of these seeds were compared: deep freeze blotter test, blotter test with mannitol, blotter test with polyethylene glycol, agar tests on potato-dextrose-agar (PDA) and on reduced PDA (RPDA) without seed disinfection, and agar tests on PDA and RPDA after seed disinfection. The evaluation was performed after 10 and 14 days of incubation. Results: Thirty two fungal genera were associated with the seeds. Alternaria alternata, Cladosporium spp. and Rhizopus nigricans were identified most frequently. Prolongation of incubation time favoured growth of Fusarium spp. and R. nigricans to the highest extent. Conclusions: The greatest seed infestation with fungi, especially Alternaria spp., was observed in the deep freeze blotter test followed by the blotter test with mannitol. Both of them could be recommended for further study on caraway seed health testing.

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Comparison of modified blotter and agar incubation methods for detecting fungi in Zinnia elegans seeds

Cited by 13 publications

References 0 publications

Comparison of methods for detecting fungi in Silybum marianum (L.) Gaertn. seeds

Comparison of methods for detecting fungi in Silybum marianum (L.) Gaertn. seeds

Alternaria alternata (Black Rot, Black Spot)

Incubation methods for the detection of fungi associated with caraway (Carum carvi L.) seeds

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