Comparison of monoclonal and polyclonal antibodies to cotinine in nonisotopic and isotopic immunoassays

Bjercke, Robert J.; Cook, Gary; Langone, John J.

doi:10.1016/0022-1759(87)90320-6

Cited by 31 publications

(23 citation statements)

References 5 publications

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“…The average value of total nicotine and cotinine, of the scalp hair samples are reported [141] ( Table 5). RIA (with 3 H and 125 I radionucleides) in fluid as well as solid phase systems, involving the use of monoclonal antibodies specific for cotinine, have been developed [137,138]. In the latter case, the cotinine polyglycine conjugate is passively adsorbed to the surface of 96-well plastic microtiter plates.…”

Section: Radioimmunoassaymentioning

confidence: 99%

See 1 more Smart Citation

Measuring tobacco smoke exposure: quantifying nicotine/cotinine concentration in biological samples by colorimetry, chromatography and immunoassay methods

Dhar

2004

Journal of Pharmaceutical and Biomedical Analysis

114

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Section: Radioimmunoassaymentioning

confidence: 99%

“…RIA involves the use of two radioisotopes-iodine ( 125 I) [133][134][135][136][137][138][139][140][141][142] and tritium ( 3 H). The technique is specific, sensitive, and easy to perform; it can be applied with appropriate dilution directly to the sample without taking recourse to extraction, evaporation, and concentration steps.…”

Section: Radioimmunoassaymentioning

confidence: 99%

Measuring tobacco smoke exposure: quantifying nicotine/cotinine concentration in biological samples by colorimetry, chromatography and immunoassay methods

Dhar

2004

Journal of Pharmaceutical and Biomedical Analysis

114

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“…Therefore, they used monoclonal antibodies to develop enzyme linked immunosorbent assays (ELISA) and a fluorescence immuno assay (FIA) (Benkirane et al, 1991). But, in 1987 he used polyclonal antibodies, which have shown a 100 -1000 fold higher sensitivity compared to monoclonal antibodies (Bjercke, 1987). This technique was later opposed by Feyerabend and Russelll in 1990, because they observed that chromatography techniques were more sensitive and specific compared to immunoassays.…”

Section: Salivary Cotinine Detectionmentioning

confidence: 99%

Detection of Cotinine in Passive Smokers Exposed to Environmental Tobacco Smoke

Ameer¹,

Kandiah²

2017

International Conference on Bioscience and Biotechnology

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Environmental tobacco smoke (ETS) is the main cause of second hand smoking (SHS) induced disease conditions such as chronic obstructive pulmonary disease (COPD), lung cancer, vascular cancer and other types of cancers. It was estimated by the Health and Social Care Information Center (HSCIC) in UK that in the year 2016, 16 million people worldwide between the ages of 16 -35 years died of smoking related diseases. Hence, different analytical techniques based on mass spectrometry (MS) and chromatography were developed, to detect the presence of tobacco metabolites in body fluids. These tobacco biomarkers in different body fluids could be used to correlate the level of ETS exposure. Among all the tobacco metabolites, cotinine was studied to be the most reliable biomarker, since it does not get affected by other environmental factors and it has along half-life f 17 hours. Therefore, by studying the levels of SHS, smoking cessation programs in public places like schools, parks and public transports could be reinforced in order to protect the innocent lives who are being exposed to tobacco smoke. This review mainly focuses on the detection of cotinine in saliva, serum, urine and hair. In conclusion, it was evident that cotinine detection in hair was the most reliable biological matrix to detect ETS exposure in passive smokers.

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“…Anti-nicotine mAb 402C10, an IgG2aK antibody, is specific for naturally occurring (-)-nicotine (6) and was used as immunogen for obtaining anti-idiotype mAbs (9). Nine antiidiotypes were selected that inhibited the binding of fluidphase anti-nicotine to immobilized nicotine-polylysine.…”

Section: Methodsmentioning

confidence: 99%

“…Nine antiidiotypes were selected that inhibited the binding of fluidphase anti-nicotine to immobilized nicotine-polylysine. These mAbs were extensively cloned, and one, anti-idiotype 422F11, has been extensively characterized as to ligand and receptor specificity (9).…”

Section: Methodsmentioning

confidence: 99%

Characterization of a purified nicotinic receptor from rat brain by using idiotypic and anti-idiotypic antibodies.

Abood¹,

Langone²,

Bjercke³

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

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The availability of an anti-nicotine monoclonal antibody has made it possible to further establish the nature of the nicotine recognition proteins purified from rat brain by affinity chromatography and to provide a highly sensitive assay for determining [ H]nicotine binding to the purified material.An enantiomeric analogue of nicotine, (-)-6-hydroxymethylnicotine, was used to prepare the affinity column. In addition, with the use of an anti-idiotypic monoclonal antibody, it was confirmed that the recognition site for nicotine resides on a protein complex composed of two components with molecular masses of 62 and 57 kDa. It was also demonstrated that the same two proteins could be purified by immunoaffinity chro- Although the nicotinic cholinergic receptors of the Torpedo electric organ and mammalian neuromuscular junction are similar in their molecular characteristics (see ref. 1 for review), the nature of the nicotinic site in mammalian brain and its relationship to the peripheral receptor remains problematical. A number of studies have described limited crossreactivity between antibodies against the electric organ and an a-bungarotoxin-binding site in rat brain (2-4); however, neither receptor-binding studies using [3H]nicotine and 3H-labeled cholinergic agents as ligands nor behavioral and electrophysiological studies indicate any similarity in the a-bungarotoxin and nicotine sites in rat brain. Recently, a monoclonal antibody (mAb) prepared against purified nicotinic receptors from chicken brain has been used to isolate, by immunoaffinity chromatography, a nicotinic receptor from rat brain (4). The receptor complex, which was composed of two proteins having molecular masses of 51 and 79 kDa, differs somewhat from a nicotine-binding complex purified by affinity chromatography using a nicotine analogue conjugated to epoxy-Sepharose (5).The present communication describes further studies on the characterization of the nicotinic receptor purified by affinity chromatography by making use of idiotypic mAbs (6) to nicotine as well as their anti-idiotypes. MATERIALS AND METHODSPreparation of Rat Brain Membranes. After adult male Sprague-Dawley rats were sacrificed by spinal dislocation, the whole brains were removed, washed, and homogenized with a Brinkmann Polytron in 20 vol of ice-cold 0.05 M sodium phosphate, pH 7.5. The membrane pellet obtained after centrifugation at 50,000 x g for 30 min was washed once, recentrifuged, and stored at -

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Comparison of monoclonal and polyclonal antibodies to cotinine in nonisotopic and isotopic immunoassays

Cited by 31 publications

References 5 publications

Measuring tobacco smoke exposure: quantifying nicotine/cotinine concentration in biological samples by colorimetry, chromatography and immunoassay methods

Measuring tobacco smoke exposure: quantifying nicotine/cotinine concentration in biological samples by colorimetry, chromatography and immunoassay methods

Detection of Cotinine in Passive Smokers Exposed to Environmental Tobacco Smoke

Characterization of a purified nicotinic receptor from rat brain by using idiotypic and anti-idiotypic antibodies.

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