Comparison of monoclonal biotin-avidin enzyme immunoassay and monoclonal time-resolved fluoroimmunoassay in detection of respiratory virus antigens

Scalia, Guido; Halonen, Pekka; Condorelli, Francesca; Mattila, Marja L.; Hierholzer, John C.

doi:10.1016/0928-0197(94)00050-5

Cited by 9 publications

(3 citation statements)

References 21 publications

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“…Several sandwich ELISA procedures (antigen capture ELISA) based on anti-HA and anti-NP antibodies have been suggested for the detection of influenza viruses (Chomel et al, 1989;Hornsleth and Jankowski, 1990;Scalia et al, 1995;Tkáčová and Varečková, 1996;Varečková et al, 2001;Zhang et al, 2006;He et al, 2007;Yang et al, 2008;Du et al, 2009;Yang et al, 2009). Various immunochemical properties and broad epitope specificity of MAbs analyzed in this study allowed us to develop several sandwich ELISAs for the detection of influenza A viruses including new pandemic H1N1 influenza viruses, for typing of AIV, and for differentiating HPAIV H5N1 of subclades 2.2 and 2.3.2.…”

Section: Discussionmentioning

confidence: 96%

Development of monoclonal antibodies to highly pathogenic avian influenza H5N1 virus and their application to diagnostics, prophylaxis, and therapy

Масалова¹,

Климова²,

Ev³

et al. 2011

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A panel of 17 monoclonal antibodies (MAbs) against highly pathogenic avian influenza virus (HPAIV) A/Duck/Novosibirsk/56/05 A/H5N1 (subclade 2.2) isolated in Russian Federation was developed. Immunoblot analysis showed that 12 MAbs were specific for the hemagglutinin (HA) and 5 MAbs for nucleoprotein (NP). All anti-HA MAbs were reactive in ELISA and immunofluorescence (IF) test and 10 of them were reactive in hemagglutination-inhibition (HI) and neutralization tests. Quantitative competitive ELISA revealed that anti-HA MAbs recognized at least 4 non-overlapping antigenic determinants and anti-NP MAbs recognized at least 3 non-overlapping antigenic determinants. Four sandwich ELISA procedures were developed using the obtained MAbs. These procedures are useful for 1) identification of avian, human, and swine influenza A viruses, 2) differentiation of avian influenza virus (AIV) from human and swine influenza viruses, 3) differentiation of AIV H5 from other AIV subtypes, and 4) differentiation between 2.2 and 2.3.2 subclades of H5N1 influenza viruses. Prophylactic and therapeutic efficacy of anti-HA MAbs with high neutralization activity was tested in BALB/c mice. A complete protection was achieved by single injection of MAbs (20 mg/kg) 24 hrs before challenge with 10 LD 50 of HPAIV H5N1. Therapeutic efficacy was 90% that was similar to those of Rimantadine and Tamiflu.

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Section: Discussionmentioning

confidence: 96%

Development of monoclonal antibodies to highly pathogenic avian influenza H5N1 virus and their application to diagnostics, prophylaxis, and therapy

Масалова¹,

Климова²,

Ev³

et al. 2011

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“…Finally, for 2018 and earlier dates, T 89 , labelled as "assay", is the nearest neighbour of T 64 . Some of the most relevant cross-domain documents for this set of dates are the use of a PCR assay to detect a wide range of human adenoviruses [60] and the comparison of two different immunoassays to detect respiratory virus antigens [61].…”

Section: Cross-domain Document Validationmentioning

confidence: 99%

Dynamic Topic Modelling for Exploring the Scientific Literature on Coronavirus: An Unsupervised Labelling Technique

Guillén-Pacho,

Badenes-Olmedo,

Corcho

2023

Preprint

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Purpose: The work presented in this article focusses on improving the interpretability of probabilistic topic models created from a large collection of scientific documents that evolve over time. Methods: Several time-dependent approaches based on topic models were compared to analyse the annual evolution of latent concepts in the CORD-19 corpus: Dynamic Topic Model, Dynamic Embedded Topic Model, and BERTopic. Then COVID-19 period (December 2019 - present) has been analysed in greater depth, month by month, to explore the evolution of what is written about the disease. Results: The evaluations suggest that the Dynamic Topic Model is the best choice to analyse the CORD-19 corpus. A novel topic labelling strategy is proposed for dynamic topic models to analyse the evolution of latent concepts. It incorporates content changes in both the annual evolution of the corpus and the monthly evolution of the COVID-19 disease. The generated labels are manually validated using two approaches: through the most relevant documents on the topic, and through the documents that share the most semantically similar label topics. Conclusions: The labelling enables the interpretation of topics. The novel method for dynamic topic labelling fits the content of each topic and supports the semantics of the topics.

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“…However, the final interpretation of the test results often requires expertise (Mclntosh et al, 1993). When TR-FIA and biotin-EIAs based on monoclonal antibodies and the one-incubation principle were developed, sensitivity was considerably improved and at the same time the tests became more practical, with fewer incubation and washing steps (Halonen et al, 1985;Walls et al, 1986;Hierholzer et al, 1990;Scalia et al, 1995). The format of the TR-FIA is shown in Fig.…”

Section: Solid-phase M~munoassays (Ria Eia Tr-fia)mentioning

confidence: 99%

Advances in the diagnosis of respiratory virus infections

Halonen

Herholzer

Ziegler

1996

Clinical and Diagnostic Virology

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The choice of diagnostic methods for respiratory virus infections depends on the type and location of the laboratory, the number of specimens tested, and the previous experience of the laboratory. Virus culture, whenever possible, should be the basic diagnostic method; the results, including identification of the virus, should be available no more than 24 h later than the results of rapid diagnostic tests. In small laboratories, especially in hospitals where specimen transportation is well organized, immunofluorescence may be the best choice for antigen detection with the provision that an experienced microscopist and a good UV microscope are available. If the laboratory receives a large number of specimens and has previous experience with EIAs, then biotin-EIAs or TR-FIAs may be the most practical techniques. Their advantages include the stability of the antigens in clinical samples since intact, exfoliated epithelial cells are not required, treatment of specimens is practical, testing of large numbers of specimens is possible, and reading the printed test result is less subjective than reading fluorescence microscopy. The larger role of PCR in the diagnosis of respiratory virus infections depends on future developments such as practical methods to extract DNA or RNA and to purify the extracts from nonspecific inhibitors, plus further improvements to minimize cross-contamination. Group-specific detection of enteroviruses and rhinoviruses is an example of the potential for PCR technology. In experienced laboratories. EIA IgG antibody tests should be available. Recombinant antigens may be a useful part of such assays.

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Comparison of monoclonal biotin-avidin enzyme immunoassay and monoclonal time-resolved fluoroimmunoassay in detection of respiratory virus antigens

Abstract: All monoclonal Biotin-EIAs can be optimized to the same sensitivity as TR-FIAs for the detection of respiratory viruses. Laboratories which have no TR-FIA expertise may use Biotin-EIA in the diagnosis of acute respiratory infections.

Cited by 9 publications

References 21 publications

Development of monoclonal antibodies to highly pathogenic avian influenza H5N1 virus and their application to diagnostics, prophylaxis, and therapy

Development of monoclonal antibodies to highly pathogenic avian influenza H5N1 virus and their application to diagnostics, prophylaxis, and therapy

Dynamic Topic Modelling for Exploring the Scientific Literature on Coronavirus: An Unsupervised Labelling Technique

Advances in the diagnosis of respiratory virus infections

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