Comparison of multiplex PCR, enzyme immunoassay and cell culture methods for the detection of enterotoxinogenic Bacillus cereus

Wehrle, Esther; Moravek, Maximilian; Dietrich, Richard; Burk, C. John; Didier, Andrea; Märtlbauer, Erwin

doi:10.1016/j.mimet.2009.06.013

Cited by 89 publications

(81 citation statements)

References 43 publications

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“…While, 75% of isolates from diarrhea samples carried nhe and cytk genes and 50% of isolates were positive for hbl gene. In the previous studies, it was showed that the genes of the HBL complex was generally less frequent than the NHE complex (Wehrle et al, 2009 andZhou et al, 2010) and both of them are considered to be the major virulence factor of B. cereus related to food poisoning (Stenfors-Arnesen et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Detection of B. Cereus and Some of Its Virulence Genes in Some Dairy Desserts and Children Diarrhea

El‐Zamkan¹,

Mubarak²

2017

AJVS

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Key words: B. cereus, Dairy desserts, Diarrhea and virulence genesB. cereus is an opportunistic foodborne pathogen that is found in most of the cases to be responsible for food-borne outbreaks. This study aimed to detect and quantify B. cereus in dairy desserts that locally manufactured and sold in different localities in Qena city, also to take an image about the role that is may be played by these products in causing foodborne illness by detection of B. cereus in diarrheal samples collected from the same regions from which the food samples are obtained. A total of 120 samples of ice-cream, mahallbia, rice and milk and diarrhea (30 samples each) were examined for presence of B. cereus. The results obtained in this study revealed detection of B. cereus in 55.6 and 40% of dairy dessert and diarrhea samples, respectively. B. cereus could be counted in 30, 60 and 76.7% of ice-cream, mahallbia and rice and milk samples with mean values of 3.5× 10 4 ±1.5× 10 4 , 6.3×10 5 ±1.9×10 5 and 1.4×10 7 ±6.6× 10 6 CFU/g, respectively. The possibility of involvement of theses dairy dessert samples in food poisoning cases was reinforced especially after detection the virulence genes of B. cereus isolates. It was found that 80, 44 and 24% of B. cereus isolates recovered from food samples harbored nhe, hbl and cytK genes, receptively including 55.6, 33.3 and 0% for ice-cream isolates; 83.3, 50 and 44.4% for mahallbia isolates and 86.9, 43.5 and 17.4% for rice and milk isolates. While for diarrhea samples, 75% of B. cereus isolates obtained from diarrhea samples carried nhe and cytK, respectively and 50% were found to be positive for hbl gene. Neither of the isolates that obtained from dairy dessert nor those obtained from diarrhea samples was positive for presence of ces gene.

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Section: Discussionmentioning

confidence: 99%

Detection of B. Cereus and Some of Its Virulence Genes in Some Dairy Desserts and Children Diarrhea

El‐Zamkan¹,

Mubarak²

2017

AJVS

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“…Details for the B. cereus strains used are given in Table 1. All strains lacked both hbl and cytK as demonstrated by PCR, immunoassay, and cell culture assay (25). The cytotoxic strain NVH0075/95 was isolated following a large food-poisoning outbreak in Norway (14) and produces all three components of the Nhe complex.…”

Section: Methodsmentioning

confidence: 99%

Cytotoxicity of the Bacillus cereus Nhe Enterotoxin Requires Specific Binding Order of Its Three Exoprotein Components

et al. 2010

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“…The multiplex PCR technique was used to detect the presence of the hblA, hblC, hblD, nheA, nheB, nheC and ctyK1 genes of B. cereus according to the procedure provided by Wehrle et al [22] . For our study, the analysis was a combination of two multiplex PCRs and a general PCR (PCR 1-3).…”

Section: -Detection Of B Cereus Enterotoxins Production and Toxin Typementioning

confidence: 99%

Determination of Enterotoxigenic Gene Profiles of Bacillus cereus Strains Isolated from Dairy Desserts by Multiplex PCR

Onur¹

2013

Kafkas Univ Vet Fak Derg

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The aim of this study was to investigate the presence of Bacillus cereus and to detect enterotoxigenic genes in dairy dessert samples utilising a multiplex PCR technique. A total of 100 samples, 25 keskul, 12 tavuk gogsu, 12 kazandibi, 30 supangle, 4 profiteroles and 17 sutlac were analysed. B. cereus contamination was found in 7 of 100 (7.0%) samples including 3 (25%) tavuk gogsu, 2 (6.6%) supangle, 1 (4%) keskul and 1 (5.8%) sutlac. The average number of B. cereus was between 2.0×10 1 -5.0×10 2 cfu/g in these dairy dessert samples. A total of 20 isolates were collected from the 7 positive samples. The results indicated that 30% (6/20) of B. cereus isolates contain three enterotoxic HBL complex encoding genes hblA, hblC and hblD, whereas 70% (14/20) had no hbl genes. In addition, all three enterotoxic NHE complex encoding genes, nheA, nheB and nheC, were detected in 40% (8/20), two nhe genes (nheA and nheB) were found in 45% (9/20) and one nhe gene (two nheA and one nheB) was found in 15% (3/20) of the isolates. The ctyK1 gene was not detected in any sample. The presence of B. cereus and their enterotoxigenic genes in dairy desserts may be a potential risk for public health. Keywords: B.cereus, Dairy dessert, Enterotoxin genes Sütlü Tatlılardan İzole Edilen Bacillus cereus Suşlarının Enterotoksijenik Gen Profillerinin Multipleks PCR ile Belirlenmesi ÖzetBu çalışmanın amacı sütlü tatlılarda Bacillus cereus varlığını araştırmak ve enterotoksijenik genleri multipleks PCR tekniğiyle belirlemektir. Bu amaçla 25 keşkül, 12 tavuk göğsü, 12 kazandibi, 30 supangle, 4 profiterol ve 17 sütlaç olmak üzere toplamda 100 örnek analiz edilmiştir. 3'ü tavuk göğsü (%25), 2'si supangle (%6.6), 1'i keşkül (%4) ve 1'i sütlaç (%5.8) olmak üzere toplamda 7 örnek (%7.0) B. cereus varlığı açısından pozitif bulunmuştur. Analiz edilen sütlü tatlı örneklerinde ortalama B. cereus sayısı 2.

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Comparison of multiplex PCR, enzyme immunoassay and cell culture methods for the detection of enterotoxinogenic Bacillus cereus

Cited by 89 publications

References 43 publications

Detection of B. Cereus and Some of Its Virulence Genes in Some Dairy Desserts and Children Diarrhea

Detection of B. Cereus and Some of Its Virulence Genes in Some Dairy Desserts and Children Diarrhea

Cytotoxicity of the Bacillus cereus Nhe Enterotoxin Requires Specific Binding Order of Its Three Exoprotein Components

Determination of Enterotoxigenic Gene Profiles of Bacillus cereus Strains Isolated from Dairy Desserts by Multiplex PCR

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