Comparison of multiplex polymerase chain reaction and immunoassay to detect Salmonella spp., S. Typhimurium, and S. Enteritidis in Thai chicken meat

Rodpai, Ekkarat; Moongkarndi, Primchanien; Tungrugsasut, Walla; Phisannoradej, Rangsichon; Kanarat, Sasitorn

doi:10.2306/scienceasia1513-1874.2013.39.150

Cited by 15 publications

(6 citation statements)

References 32 publications

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“…Typhimurium) and Enteritidis ( S . Enteritidis) are the most common serovars linked with human foodborne illness among the more than 2500 serovars [ 11 , 12 ]. Human S .…”

Section: Introductionmentioning

confidence: 99%

“…Several researchers reported variable prevalence rates of Salmonella infection in animal food sources in Bangladesh [9,10]. Salmonella enterica serovars Typhimurium (S. Typhimurium) and Enteritidis (S. Enteritidis) are the most common serovars linked with human foodborne illness among the more than 2500 serovars [11,12]. Human S. Enteritidis cases are mostly associated with the consumption of contaminated eggs and poultry meat, while S. Typhimurium cases with the consumption of contaminated pork, poultry, and beef [13,14].…”

Section: Introductionmentioning

confidence: 99%

“…Human S. Enteritidis cases are mostly associated with the consumption of contaminated eggs and poultry meat, while S. Typhimurium cases with the consumption of contaminated pork, poultry, and beef [13,14]. A diverse prevalence of Salmonella enterica serovars has been reported in many countries across the globe among the animal products and by products [12,15,16]. It is universal fact that human Salmonella illness are related with numerous diverse kinds of food, including animal origin food and food products [2,17].…”

Section: Introductionmentioning

confidence: 99%

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Virulence and Antimicrobial Resistance Profiles of Salmonella enterica Serovars Isolated from Chicken at Wet Markets in Dhaka, Bangladesh

et al. 2021

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Virulent and multi drug resistant (MDR) Salmonellaenterica is a foremost cause of foodborne diseases and had serious public health concern globally. The present study was undertaken to identify the pathogenicity and antimicrobial resistance (AMR) profiles of Salmonellaenterica serovars recovered from chicken at wet markets in Dhaka, Bangladesh. A total of 870 cecal contents of broiler, sonali, and native chickens were collected from 29 wet markets. The overall prevalence of S. Typhimurium, S. Enteritidis, and untyped Salmonella spp., were found to be 3.67%, 0.57%, and 1.95% respectively. All isolates were screened by polymerase chain reaction (PCR) for eight virulence genes, namely invA, agfA, IpfA, hilA, sivH, sefA, sopE, and spvC. S. Enteritidis isolates carried all virulence genes whilst S. Typhimurium isolates carried six virulence genes except sefA and spvC. A diverse phenotypic and genotypic AMR pattern was found. Harmonic descending trends of resistance patterns were observed among the broiler, sonali, and native chickens. Interestingly, virulent and MDR Salmonella enterica serovars were found in native chicken, although antimicrobials were not used in their production cycle. The research findings anticipate that virulent and MDR Salmonella enterica are roaming in the wet markets which can easily anchor to the vendor, consumers, and in the food chain.

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“…Typhimurium) and Enteritidis ( S . Enteritidis) are the most common serovars linked with human foodborne illness among the more than 2500 serovars [ 11 , 12 ]. Human S .…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Virulence and Antimicrobial Resistance Profiles of Salmonella enterica Serovars Isolated from Chicken at Wet Markets in Dhaka, Bangladesh

et al. 2021

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“…21 As such, several screening methods such as nucleic acid-based assays and immunology-based assays have been examined for a rapid detection of Salmonella in the recent decades. 20 , 22 , 23 , 24 In earlier studies, lipopolysaccharides (LPS) present on the bacterial surface were used in the ELISA-assay for the detection of Salmonella , but the problem with lipopolysaccharides is the cross-reactivity with other gram-negative bacteria. 25 , 26 , 27 Therefore, flagellin (FliC) was sought as an alternative for rapid diagnosis of Salmonella and, during the last decade, several studies have used it for the detection of Salmonella .…”

Section: Discussionmentioning

confidence: 99%

Production of recombinant flagellin to develop ELISA-based detection of Salmonella Enteritidis

Mirhosseini

Fooladi

Amani

et al. 2017

Brazilian Journal of Microbiology

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Food-borne diseases, caused by the pathogenic bacteria, are highly prevalent in the world. Salmonella is one of the most important bacterial genera responsible for this. Salmonella Enteritidis (SE) is one of the non-typhoid Salmonellae that can be transmitted to human from poultry products, water, and contaminated food. In recent years, new and rapid detection methods such as enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) have been developed. In this study, recombinant FliC (rFliC) was produced to be used as an antigen. The immunization was conducted in mice with the purified recombinant FliC (rFliC). The mice were subcutaneously immunized with rFliC and elicited significant rFliC specific serum IgG antibodies. An indirect ELISA system was established for the detection of Salmonella Enteritidis. Our results confirmed that the recombinant flagellin can be one of the excellent indicators for the detection of Salmonella Enteritidis.

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“…Tracking Salmonella Enteritidis in the Genomics Era: Clade Definition Using a SNP-PCR Assay… DOI: http://dx.doi.org/10.5772/intechopen.98309 operon [42]. Multiplex PCR assays that are able to detect and distinguish among multiple serovars have also been developed by including serovar-specific gene targets such as STM4449 (Typhimurium [43]), STM 4497 (Typhimurium [44], fliC (Typhimurium [45]), sdfI (Enteritidis [46]) and sefA [29]. Recent work by Nadin-Davis and colleagues showed that many of the previously identified serovar specific markers were shared by other serovars especially sefA and fliC while highlighting the limitation with the use of a plasmid encoded target [47].…”

Section: Identification Of Salmonella Enteritidismentioning

confidence: 99%

Tracking Salmonella Enteritidis in the Genomics Era: Clade Definition Using a SNP-PCR Assay and Implications for Population Structure

Ogunremi¹,

Gao²,

Slowey³

et al. 2021

Salmonella Spp. - A Global Challenge

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Salmonella enterica serovar Enteritidis (or Salmonella Enteritidis, SE) is one of the oldest members of the genus Salmonella, based on the date of first description and has only gained prominence as a significant bacterial contaminant of food over the last three or four decades. Currently, SE is the most common Salmonella serovar causing foodborne illnesses. Control measures to alleviate human infections require that food isolates be characterized and this was until recently carried out using Pulsed-Field Gel Electrophoresis (PFGE) and phage typing as the main laboratory subtyping tools for use in demonstrating relatedness of isolates recovered from infected humans and the food source. The results provided by these analytical tools were presented with easy-to-understand and comprehensible nomenclature, however, the techniques were inherently poorly discriminatory, which is attributable to the clonality of SE. The tools have now given way to whole genome sequencing which provides a full and comprehensive genetic attributes of an organism and a very attractive and superior tool for defining an isolate and for inferring genetic relatedness among isolates. A comparative phylogenomic analysis of isolates of choice provides both a visual appreciation of relatedness as well as quantifiable estimates of genetic distance. Despite the considerable information provided by whole genome analysis and development of a phylogenetic tree, the approach does not lend itself to generating a useful nomenclature-based description of SE subtypes. To this end, a highly discriminatory, cost-effective, high throughput, validated single nucleotide based genotypic polymerase chain reaction assay (SNP-PCR) was developed focussing on 60 polymorphic loci. The procedure was used to identify 25 circulating clades of SE, the largest number so far described for this organism. The new subtyping test, which exploited whole genome sequencing data, displays the attributes of an ideal subtyping test: high discrimination, low cost, rapid, highly reproducible and epidemiological concordance. The procedure is useful for identifying the subtype designation of an isolate, for defining the population structure of the organism as well as for surveillance and outbreak detection.

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Comparison of multiplex polymerase chain reaction and immunoassay to detect Salmonella spp., S. Typhimurium, and S. Enteritidis in Thai chicken meat

Cited by 15 publications

References 32 publications

Virulence and Antimicrobial Resistance Profiles of Salmonella enterica Serovars Isolated from Chicken at Wet Markets in Dhaka, Bangladesh

Virulence and Antimicrobial Resistance Profiles of Salmonella enterica Serovars Isolated from Chicken at Wet Markets in Dhaka, Bangladesh

Production of recombinant flagellin to develop ELISA-based detection of Salmonella Enteritidis

Tracking Salmonella Enteritidis in the Genomics Era: Clade Definition Using a SNP-PCR Assay and Implications for Population Structure

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