Comparison of myocardial ischemic/hypoxic staining techniques for evaluating the alleviation of exhaustive exercise-induced myocardial injury by exercise preconditioning

Huang, Yue; Pan, Shan; Guo, Yuan-Pan; Wang, Jiayin; Wan, Dong-Feng; Chen, Tianran; Yuan, Jun

doi:10.1007/s10735-021-09958-0

Cited by 5 publications

(5 citation statements)

References 16 publications

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“…There are many ways to detect myocardial ischemic/hypoxic injury. cTnI is a blood biomarker with high specificity for detecting myocardial ischemic/hypoxic injury 22 , 35 . HBFP staining is a specific staining method for ischemia/hypoxia that is often used to detect early myocardial ischemic/hypoxic injury 36 .…”

Section: Discussionmentioning

confidence: 99%

“…HBFP staining is a specific staining method for ischemia/hypoxia that is often used to detect early myocardial ischemic/hypoxic injury 36 . Normal cardiomyocytes are stained yellow by HBFP staining, but ischemic/hypoxic cardiomyocytes are stained scarlet 22 . In addition, the ST segment and T wave are ECG indexes that indicate myocardial ischemia/hypoxia; if this condition is present, the ST segment is obviously elevated or decreased, and the T wave is low, bidirectional or inverted 15 .…”

Section: Discussionmentioning

confidence: 99%

“…Hematoxylin-basic fuchsin-picric acid (HBFP) staining is an ischemia/hypoxia stain that can show high-intensity exercise-induced myocardial ischemic/hypoxic injury, with injured cardiomyocytes being stained scarlet 4 , 22 . ST segment and T wave reflect myocardial ischemia/hypoxia from the perspective of electrophysiology, while HBFP staining reflects the degree of myocardial ischemia/hypoxia from a histological perspective.…”

Section: Introductionmentioning

confidence: 99%

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Exercise preconditioning improves electrocardiographic signs of myocardial ischemic/hypoxic injury and malignant arrhythmias occurring after exhaustive exercise in rats

Guo

2022

Sci Rep

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Exercise preconditioning (EP) has a good myocardial protective effect. This study explored whether EP improves electrocardiographic (ECG) signs of myocardial ischemic/hypoxic injury and the occurrence of malignant arrhythmia after exhaustive exercise. A total of 120 male SD rats were randomly divided into the control group (group C), early exercise preconditioning group (group EEP), late exercise preconditioning group (group LEP), exhaustive exercise group (group EE), early exercise preconditioning + exhaustive exercise group (group EEP + EE) and late exercise preconditioning + exhaustive exercise group (group LEP + EE). Changes in heart rate (HR), ST segment, T wave and QT corrected (QTc) intervals on ECG; hematoxylin-basic fuchsin-picric acid (HBFP) staining; and cTnI levels were used to study myocardial injury and the protective effect of EP. Compared with those in group C, the levels of plasma markers of myocardial injury, HBFP staining and ECG in group EE were significantly increased (P < 0.05). Compared with those in group EE, the levels of plasma markers of myocardial injury, HBFP staining and ECG in group EEP + EE and group LEP + EE were significantly decreased (P < 0.05). The results suggested that EP improved ECG signs of myocardial ischemic/hypoxic injury and malignant arrhythmias that occur after exhaustive exercise. The ST segment and T wave could also serve as indexes for evaluating exhaustive exercise-induced myocardial ischemia/hypoxia.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Exercise preconditioning improves electrocardiographic signs of myocardial ischemic/hypoxic injury and malignant arrhythmias occurring after exhaustive exercise in rats

Guo

2022

Sci Rep

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“…At least 400 mitochondria were randomly selected from five regions in each sample. Image J software was used to analyse mitochondrial morphology changes and cross‐sectional areas (mitochondrial cross‐sectional integration categories were classified according to three ranges of <0.6 μm 2 , 0.6–1.0 μm 2 , >1.0 μm 2 ) 20,21 . Further mitochondrial analysis was performed by calculating the percentage of mitochondria in a given field that fell into these three size categories.…”

Section: Methodsmentioning

confidence: 99%

Endoplasmic reticulum stress induced by turbulence of mitochondrial fusion and fission was involved in stressed cardiomyocyte injury

Zhang,

Li,

Zhu

et al. 2023

J Cellular Molecular Medi

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Mitochondria are sensitive organelles that sense intrinsic and extrinsic stressors and maintain cellular physiological functions through the dynamic homeostasis of mitochondrial fusion and fission. Numerous pathological processes are associated with mitochondrial fusion and fission disorders. However, the molecular mechanism by which stress induces cardiac pathophysiological changes through destabilising mitochondrial fusion and fission is unclear. Therefore, this study aimed to investigate whether the endoplasmic reticulum stress signalling pathway initiated by the turbulence of mitochondrial fusion and fission under stressful circumstances is involved in cardiomyocyte damage. Based on the successful establishment of the classical stress rat model of restraint plus ice water swimming, we measured the content of serum lactate dehydrogenase. We used haematoxylin–eosin staining, special histochemical staining, RT‐qPCR and western blotting to clarify the cardiac pathology, ultrastructural changes and expression patterns of mitochondrial fusion and fission marker proteins and endoplasmic reticulum stress signalling pathway proteins. The results indicated that mitochondrial fusion and fission markers and proteins of the endoplasmic reticulum stress JNK signalling pathway showed significant abnormal dynamic changes with the prolongation of stress, and stabilisation of mitochondrial fusion and fission using Mdivi‐1 could effectively improve these abnormal expressions and ameliorate cardiomyocyte injury. These findings suggest that stress could contribute to pathological cardiac injury, closely linked to the endoplasmic reticulum stress JNK signalling pathway induced by mitochondrial fusion and fission turbulence.

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“…На окрашенных ГОФП-методом микропрепаратах выраженность повреждения миокарда оценивали по полуколичественной шкале (10-кратное увеличение объектива микроскопа; по 10 полям зрения) [17]: 0 баллов -интактный миокард, 1 балл -минимальная выраженность (< 26 % миокарда), 2 балла -от минимального до умеренного уровня (26-50 % миокарда), 3 балла -умеренный уровень (51-75 % миокарда), 4 балла -тяжелое повреждение (> 75 % миокарда). Также рассчитывали удельную площадь повреждения миокарда как соотношение интегральной оптической плотности фуксинофилии к площади повреждения миокарда (10-кратное увеличение объектива микроскопа; по 10 полям зрения) [18].…”

Section: материал и методыunclassified

Influence of trimetazidine on myocardium energy balance during chemotherapy with doxorubicin and cyclophosphamide

Avagimyan

2022

Sibirskij nauchnyj medicinskij zhurnal

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Aim of the study was to assess the degree of myocardial ischemia in rats on chronic in vivo model, with a simultaneous assessment of justification for the use of trimetazidine. Material and methods. The object of the study was 120 male inbred Wistar rats, randomly divided into 4 equal groups: group 1 – control (administration of 0.9% sodium chloride solution 3 times a week); group 2 – simulation of the AC chemotherapy regimen by intraperitoneal administration of doxorubicin hydrochloride at a single dose of 2.5 mg/kg and cyclophosphamide monohydrate at a single dose of 25 mg/kg 3 times a week; group 3 – simulation of the AC chemotherapy regimen with additional administration of trimetazidine dihydrochloride daily by intragastric gavage at a single dose of 3.0 mg/kg; group 4 – administration of trimetazidine dihydrochloride. The study has been carried out for two weeks. An Olympus IX51 microscope was used to assess the changes. Staining was carried out by the HBFP method (hematoxylin + basic fuchsin + picronic acid). Results and discussion. In group 2, on the background of AC chemotherapy, the level of fuchsinophilia in myocardial tissue was 87.2 and 90.9 % higher (p < 0.05) than in groups 1 and 4, respectively, the specific area of damage was 170.8 and 167. 5 %, respectively (p < 0.05). In group 3, the severity of fuchsinophilia and the specific area of myocardial damage were statistically significantly less (by 26.3 and 36.5 %, p < 0.05) than in group 2. Conclusions. Trimetazidine is a pathogenetically effective drug that protects the myocardium from damage associated with AC chemotherapy.

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Comparison of myocardial ischemic/hypoxic staining techniques for evaluating the alleviation of exhaustive exercise-induced myocardial injury by exercise preconditioning

Cited by 5 publications

References 16 publications

Exercise preconditioning improves electrocardiographic signs of myocardial ischemic/hypoxic injury and malignant arrhythmias occurring after exhaustive exercise in rats

Exercise preconditioning improves electrocardiographic signs of myocardial ischemic/hypoxic injury and malignant arrhythmias occurring after exhaustive exercise in rats

Endoplasmic reticulum stress induced by turbulence of mitochondrial fusion and fission was involved in stressed cardiomyocyte injury

Influence of trimetazidine on myocardium energy balance during chemotherapy with doxorubicin and cyclophosphamide

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