“…There are several methods to calculate N of a naturally ventilated greenhouse Boulard, 2006 . The methods include the tracer gas method with CO 2 Okada and Takakura, 1973;Nederhoff et al, 1985;Boulard and Draoui, 1995 , the energy balance method Kozai et al, 1980;Fernandez and Bailey, 1993 , the water balance method Takakura et al, 2009Takakura et al, , 2017 , and the methods based on aerodynamic Kozai et al, 1980;Sase et al, 1980;Boulard and Baille, 1995;Boulard et al, 1996;Kittas et al, 1997or neural network Seginer et al, 1994Boulard et al, 1997 models. Among them, we have recently shown the potential availability of the NN models with internal and external environmental data and the extent of ventilator openings as the model inputs for real-time N estimation for a naturally ventilated uncropped greenhouse Matsuda et al, 2019 . Using data collected in an uncropped experimental greenhouse in the winter, NN models were trained and validated according to 10-fold cross-validation. The NN models tended to exhibit a higher accuracy in estimating N of the greenhouse than the aerodynamic and empirical models.…”