Comparison of nocturnal and diurnal metabolomes of rose flowers and leaves

Zumsteg, Julie; Bossard, Elodie; Gourguillon, Lorène; Villette, Claire; Heintz, Dimitri

doi:10.1007/s11306-023-02063-1

Cited by 1 publication

(1 citation statement)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among them, MS is considered the most informative technique for component identification and has been widely adopted in plant sciences for its high resolving power and sensitivity [ 4 ], such as gas chromatography-MS (GC-MS) and liquid chromatography-MS (LC-MS). Zumsteg et al used reversed-phase ultrahigh-performance LC coupled with electrospray ionization quadrupole time-of-flight (TOF) MS or triple-quadrupole mass spectrometry (TQ MS/MS) for comparison of nocturnal and diurnal metabolomes; they compared the accurate mass spectra with those in databases for metabolite annotation [ 5 ]. Chevalier et al developed a strategy to locate the fluorescence of prenylated anthranoid in living plant cells and discriminate it from that of the other metabolites; they combined spectral imaging, confocal microscopy, and non-targeted metabolomics using mass spectrometry [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

In-Situ Metabolic Profiling of Different Kinds of Rheum palmatum L. by Laser Desorption–Dielectric Barrier Discharge Ionization Mass Spectrometry Imaging

Xiao,

Guan,

et al. 2024

Metabolites

View full text Add to dashboard Cite

With its high resolving power and sensitivity, mass spectrometry is considered the most informative technique for metabolite qualitation and quantification in the plant sciences. However, the spatial location information, which is crucial for the exploration of plant physiological mechanisms, is lost. Mass spectrometry imaging (MSI) is able to visualize the spatial distribution of a large number of metabolites from the complex sample surface in a single experiment. In this paper, a flexible and low-cost laser desorption–dielectric barrier discharge ionization-MSI (LD-DBDI-MSI) platform was constructed by combining an LD system with an in-line DBDI source, a high-precision sample translation stage, and an ambient mass spectrometer. It can be operated at a spatial resolution of 20 μm in an atmospheric environment and requires minimal sample preparation. This study presents images of in-situ metabolic profiling of two kinds of plants from different origins, a wild and a farmed Rheum palmatum L. From the screen of these two root sections, the wild one presented five more endogenous molecules than the farmed one, which provides information about the differences in metabolomics.

show abstract

Section: Introductionmentioning

confidence: 99%