Comparison of Np-TLC and Rp-TLC With Densitometry to Quantitative Analysis of Ibuprofen in Pharmaceutical Preparations

Pyka, Alina; Bocheńska, Paulina

doi:10.1080/10826071003684372

Cited by 3 publications

(4 citation statements)

References 6 publications

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“…These results indicate that Lux silica gel plates with increased brightness F 254 are a good alternative to conventional silica gel plates 60F 254 and allowed to obtain low LOD and LOQ of ibuprofen using toluene:ethyl acetate:glacial acetic acid in a volume composition of 27:21:2. These chromatographic conditions have been reported to improve the sensitivity of the TLC-densitometric method for the analysis of ibuprofen in relation to previously reported work [16][17][18]. Summarizing results obtained with the normal-phase system, it can be stated that besides the mobile phase composition, certain differences of the layer thickness of silica gel and the presence of a concentration zone influence the chromatographic analysis of ibuprofen, i.e., the quality of chromatograms (spot width and R f value) as well as on the levels of detection and quantification.…”

Section: Discussionmentioning

confidence: 67%

“…This work confirmed also the utility of a popular stationary phase, namely silica gel 60F 254 plates in normal phase thin-layer chromatography (NP-TLC) and silica gel RP-18F 254 plates in reversed-phase thin-layer chromatography (RP-TLC) with LOD = 0.60 µg/spot for NP-TLC and LOD = 1.000 µg/spot for RP-TLC. The LOQ was 12.50 µg/spot in both cases [18].…”

Section: Introductionmentioning

confidence: 88%

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Estimating Limits of Detection and Quantification of Ibuprofen by TLC-Densitometry at Different Chromatographic Conditions

2020

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Ibuprofen is one of the best-known nonsteroidal anti-inflammatory and analgesic drugs. Following the previous work, the current study is focused on estimating the effect of different chromatographic conditions on the sensitivity of thin-layer chromatography in combination with UV densitometry, i.e., the detection and quantification of ibuprofen in a wide range of its concentrations including the lowest limits of detection (LOD) and quantification (LOQ). For this purpose, a reliable and easy-to-use calculation procedure for LOD and LOQ determination is presented in this work. In addition, the impact of type plates and mobile phase composition on the LOD and LOQ, respectively, of this active substance is accurately described. The results of detection and the quantification level of ibuprofen obtained under applied chromatographic conditions confirmed the utility of silica gel plates as well as silica gel bonded phases (i.e., reversed-phase (RP) plates) in the thin-layer chromatography (TLC)-densitometric analysis of ibuprofen at a low level, i.e., from several nanograms (below one microgram) to a few micrograms/spot. Among all chromatographic systems tested, the best are those consisting of silica gel 60F254 plates with concentrating zone (1.05583) and the mixture of n-hexane:ethyl acetate:glacial acetic acid in ratio 36:12:2 (v/v/v) as well as RP plates, i.e., RP-18F254 aluminum plates developed with methanol:water in volume composition 40:10 (v/v). These chromatographic systems allowed quantifying ibuprofen in the amount of 0.229 µg/spot and 0.228 µg/spot, which is less than 1 µg/spot. It can be stated that different chromatographic systems in combination with UV densitometric scanning at 224 nm proposed in this work can be successfully applied for the cost-effective and sensitive determination of ibuprofen as a widely used drug component as well as a residual in domestic wastewater. It was found that the modification of silica gel as well as the layer thickness of unmodified or modified silica gel 60 can influence the quality of chromatograms and the detection/quantification of ibuprofen in both normal phase (NP) and RP systems. Therefore, to obtain the best possible LOD and LOQ values of ibuprofen with precoated layers, suitable mobile phase and chromatographic plates are required.

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Section: Discussionmentioning

confidence: 67%

Section: Introductionmentioning

confidence: 88%

Estimating Limits of Detection and Quantification of Ibuprofen by TLC-Densitometry at Different Chromatographic Conditions

2020

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“…Tom 74 • nr 11 • 2018 spektrodensytogramów badanych substancji. Podobnie w innych pracach wykazaliśmy również, że porównanie widm wzorca danej substancji i jej odpowiednika występującego w leku może być podstawą badań jego tożsamości [6,7].…”

Section: Wstępunclassified

Application of spectrophotometry and densitometry to spectra evaluation of naproxen in UV-VIS range

Pyka¹,

Parys²,

Dołowy³

2018

Farm Pol.

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“…RP-TLC (reversed-phase thin layer chromatography) has been successfully applied for analysis of many drugs as Losartan [20], Oral Antidiabetic drugs [21][22], Ibuprofen [23],…”

Section: Molecular Formula C21h30brno4mentioning

confidence: 99%

Simultaneous Determination of Hyoscine N- Butyl Bromide and Paracetamol by RP-TLC Spectrodensitometric Method

Ali

Gamal

Abdelkawy

2013

BJPR

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Aims: A simple RP-TLC Spectrodensitometric method was developed for determination of Hyoscine N-Butyl Bromide (HBB) and Paracetamol (PAR) either in bulk powder or in their pharmaceutical preparation. Study Design: Validation study. Methodology: In this method, HBB and PAR were separated on RP-18 W/ UV 254 TLC plates using developing mobile phase consisting of methanol: citrate buffer (pH=1.5): triflouroacetic acid (70:30:0.1, by volume) at room temperature. Experimental conditions such as band size, slit width, different developing systems and scanning wavelength were carefully studied and the optimum conditions were selected. The obtained bands were then scanned at 210 nm. The two drugs were satisfactorily resolved with R F 0.60 ± 0.02 for HBB and 0.81 ± 0.02 for PAR. The validation of spectrodensitometric method was done regarding linearity, accuracy, precision, and specificity. Results: Linearity of the proposed methods was evaluated and it was found to lie within the concentration range of 2.0-12.0 µg.band -1 for HBB and 2.0-14.0 µg.band -1 for PAR.

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Comparison of Np-TLC and Rp-TLC With Densitometry to Quantitative Analysis of Ibuprofen in Pharmaceutical Preparations

Cited by 3 publications

References 6 publications

Estimating Limits of Detection and Quantification of Ibuprofen by TLC-Densitometry at Different Chromatographic Conditions

Estimating Limits of Detection and Quantification of Ibuprofen by TLC-Densitometry at Different Chromatographic Conditions

Application of spectrophotometry and densitometry to spectra evaluation of naproxen in UV-VIS range

Simultaneous Determination of Hyoscine N- Butyl Bromide and Paracetamol by RP-TLC Spectrodensitometric Method

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