Comparison of NS-1 Antigen Detection by ICT and ELISA  for Evaluating Acute Dengue

Kumar, Suresh; Sheethal, S

doi:10.20546/ijcmas.2018.702.434

Cited by 2 publications

(3 citation statements)

References 15 publications

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“…A confirmed acute dengue case was defined by the detection of the DENV NS1 protein alone or DENV NS1 protein with IgM antibodies or DENV NS1 protein with IgG antibodies against DENV in plasma samples of children with fever illness and at least one of the following symptoms: head ache, joint pain, back ache, abdominal pain, vomiting, fatigue, anorexia and diarrhea [26, 31, 32].…”

Section: Methodsmentioning

confidence: 99%

“…NS1 antigen is detectable in blood from first day after the onset of fever up to day 7, and still detectable in the presence of IgM antibodies, and when viral RNA is negative by RT-PCR [13, 21]. Simultaneous detection of NS1protein and IgM antibodies markers is useful to diagnose acute dengue infection [7, 25, 26]. This study therefore aims to determine the frequency of acute dengue infection among febrile children under 15 years attending hospitals in some selected areas of Cameroon.…”

Section: Introductionmentioning

confidence: 99%

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A cross-sectional study of acute dengue infection in paediatric clinics in Cameroon

et al. 2019

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Background Dengue fever is the world’s fastest spreading mosquito borne viral infection. It is prevalent throughout both subtropical and tropical region, and affects over 128 countries. Dengue virus (DENV) infection poses a serious global public health challenge to three billion people, resulting in approximately 200 million cases of morbidity and 50,000 cases of mortality annually. In Cameroon like in most sub-Saharan African countries, DENV infection occur concurrently with other infectious diseases whose symptoms often overlap, rendering differential diagnosis challenging. This study aims at determining the frequency of acute dengue among febrile children under 15 years attending hospitals in some areas of Cameroon. Methods A total of 961 children under the age of 15 were recruited in a cross-sectional study using systematic sampling technique and by selecting each subject out of the three. The study was conducted in 10 public health centers in Cameroon. Demographic data and risk factors of the subjects were obtained using well-structured questionnaires. Dengue virus NS1 antigen, IgM and IgG were analysed using a Tell me fast® Combo Dengue NS1-IgG/IgM Rapid Test. An in-house ELISA test for dengue specific IgM antibody was equally performed for confirmation. Descriptive statistical analysis was performed using Graph pad version 6.0. Results A prevalence of 6.14% acute dengue virus infection was observed among children with febrile illness with a significant difference ( p = 0.0488) between males (4.7%) and females (7.7%). In addition, children who reportedly were unprotected from vectors, showed a comparatively higher prevalence of the disease seropositivity than those practicing protective measures. Conclusion DENV infection therefore is an important cause of fever among children in Cameroon. Thus, there is a need to include differential screening for DENV infections as a tool in the management of fever in children in the country. Electronic supplementary material The online version of this article (10.1186/s12889-019-7252-9) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A cross-sectional study of acute dengue infection in paediatric clinics in Cameroon

et al. 2019

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“…Kumar et al and Chakraverti et al found no significant difference in sensitivity and specificity between ICT and ELISA tests for detection of dengue infection. 46,47 Serum is the most common sample type. In DENV infections the Dengue Virus NS1 ELISA is an important early screening test, which is possible from day 1 to approximately day 18 after onset of symptoms that helps to minimize the diagnostic gap between RT-PCR and antibody positivity.…”

Section: Isothermal Amplification Methodmentioning

confidence: 99%

Dilemma in laboratory diagnosis of Dengue, Chikungunya and Zika viruses

Ahmed¹,

Mahboob²,

Mamun³

et al. 2019

Bangla J Med

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Dengue, Chikungunya and Zika are the vector-borne diseases that constitute a potential epidemiological risk due to the recent increase in cases, complications, and severity. The co-circulation of the three diseases is a matter of public health interest due to their transmission by the same vector as well as the increase in the number of cases of severe dengue hemorrhagic fever, post-chikungunya chronic joint disease and microcephaly related to Zika virus. Therefore, it is important to be familiar with the various clinical presentations and laboratory methods to make the differential diagnosis, start appropriate treatment, and prevent the associated complications. Bangladesh J Medicine July 2019; 30(2) : 83-92

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Comparison of NS-1 Antigen Detection by ICT and ELISA for Evaluating Acute Dengue

Cited by 2 publications

References 15 publications

A cross-sectional study of acute dengue infection in paediatric clinics in Cameroon

A cross-sectional study of acute dengue infection in paediatric clinics in Cameroon

Dilemma in laboratory diagnosis of Dengue, Chikungunya and Zika viruses

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