Comparison of NSAIDs activity in COX-2 expressing and non-expressing 2D and 3D pancreatic cancer cell cultures

Čeponytė, Ugnė; Paškevičiūtė, Miglė; Petrikaitė, Vilma

doi:10.2147/cmar.s163747

Cited by 35 publications

(18 citation statements)

References 33 publications

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“…It was observed that consumption of NSAIDs can lead to high incidence of gastrointestinal irritation due to the development of life-threatening gastrointestinal ulcers and also causes abnormal renal physiology. COX-2 is overexpressed in numerous premalignant and malignant lesions con rmed the signi cant correlation between the COX-2 inhibition activity of non steroidal anti-in ammatory drugs (NSAIDs) and their antitumor e cacies (Ceponyte et al 2018). Thus, celecoxib (CXB), a selective COX-2 inhibitor, showed promising results in the prevention tumor cell growth and suppress tumor angiogenesis and metastasis Xu et al 2011).…”

Section: Discussionmentioning

confidence: 94%

Production Optimization and In Vitro Evaluation of Anti-Proliferative, Anti-Oxidant and Anti-Inflammatory Potential of the Antibacterial Peptide MAFP9

Raghavan

Pannippara²,

Kesav

et al. 2021

Preprint

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Peptides are important natural bioactive agents obtained from various sources including microbes, plants and animals. The present study involved the optimization and physicochemical characterization of an antibacterial peptide MFAP9 produced by a marine fungus Aspergillus fumigatus BTMF9 and its vitro evaluation of antiproliferative, antioxidant and anti-inflammatory potential. Antiproliferative activity was performed using A549 lung carcinoma and normal L929 fibroblast cell lines employing MTT assay method. Antioxidant activity was assessed using DPPH assay and Cox2 inhibition assay of peptide treated LPS stimulated THP1 cell lines was used for anti-inflammatory studies. The results of in vitro antiproliferative activity assessment showed that MFAP9 was cytotoxic with an IC50 value of 29 µg/mL towards A549 cells. Fluorescence microscopic analysis revealed apoptotic features such as chromatin condensation and alterations in the size and the shape of cells after treatment with MFAP9 at a concentration 50 µg/mL. The DPPH radical scavenging activity of MFAP9 exhibits reasonably higher scavenging activity compared to that of standard antioxidant at same concentration level. Moreover, MFAP9 showed significant anti-inflammatory activities. Time course experiment was conducted with the Czapek- Dox medium and optimized the rate of MFAP9 production. The study on various physico-chemical parameters on the stability revealed that the maximum active temperature of MAFP9 is 50oC, stable in basic pH and 1 mM concentration of Ni2+, Cd2+ and Mg2+ promotes the peptide activity. Hopefully, by employing the advancements in proteomics, bioinformatics, and modification strategies, MFAP9 could emerge as novel promising therapeutic drug in future for various clinical applications.

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Section: Discussionmentioning

confidence: 94%

Production Optimization and In Vitro Evaluation of Anti-Proliferative, Anti-Oxidant and Anti-Inflammatory Potential of the Antibacterial Peptide MAFP9

Raghavan

Pannippara²,

Kesav

et al. 2021

Preprint

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“…Uncontrolled migration of cancer cells is the first step towards invasion and metastasis [48] . Since many NSAIDs do possess anti‐cancer activity [49] and our main goal of the present work is to develop supramolecular metallogels for drug delivery applications, we decided to evaluate anti‐cancer behaviour of the metallogelator complexes i. e. 3‐PyMEC , 3‐PyDIC , 4‐PyNAP and 4‐PyMEC on a highly aggressive human breast cancer cell line MDA‐MB‐231 by MTT assay (for detail, see experimental). MTT assay data revealed that 3‐PyMEC possessed the best anti‐cancer property against the cell line amongst the gelator complexes studied herein; nearly 96% cancer cell death was observed at a concentration of 35 μM of 3‐PyMEC (Figure S23).…”

Section: Resultsmentioning

confidence: 99%

Designing Metallogelators Derived from NSAID‐based Zn(II) Coordination Complexes for Drug‐Delivery Applications

Mondal

Dastidar

2020

Chemistry An Asian Journal

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A crystal engineering approach has been invoked to design a new series of eight Zn(II) coordination complexes derived from various non-steroidal anti-inflammatory drugs (NSAIDs), namely diclofenac (DIC), ibuprofen (IBU), naproxen (NAP), flufenamic acid (FLU) and meclofenamic acid (MEC), and two co-ligands, namely N-phenyl-3-pyridylamide (3-Py) and N-phenyl-4-pyridylamide (4-Py), and Zn(NO 3) 2 as potential supramolecular gelators. Half of the coordination complexes thus synthesized were able to form aqueous gels (MG-3-PyMEC, MG-3-PyDIC, MG-4-PyNAP and MG-4-PyMEC). Single crystal structures of all eight complexes revealed that they possessed a gelation-inducing 1D hydrogen-bonded network including amide…amide synthon in some cases, which supported strongly the design principles based on which these complexes were synthesized. Interestingly, one such metallogelator complex, namely 3-PyMEC, showed an intriguing anticancer property against a human breast cancer cell line (MDA-MB-231), as revealed by both MTT and cell migration assays.

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“…The potential cytotoxic effect of Iris extracts on certain cell lines was determined by a MTT viability assay as described before [ 87 ]. Details can be found in the Supplementary Materials section .…”

Section: Methodsmentioning

confidence: 99%

Qualitative and Quantitative Analysis of Ukrainian Iris Species: A Fresh Look on Their Antioxidant Content and Biological Activities

et al. 2020

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The major groups of antioxidant compounds (isoflavonoids, xanthones, hydroxycinnamic acids) in the rhizome methanol extracts of four Ukrainian Iris sp. (Iris pallida, Iris hungarica, Iris sibirica, and Iris variegata) were qualitatively and quantitatively analyzed using HPLC-DAD and UPLC-MS/MS. Gallic acid, caffeic acid, mangiferin, tectoridin, irigenin, iristectorigenin B, irisolidone, 5,6-dihydroxy-7,8,3′,5′-tetramethoxyisoflavone, irisolidone-7-O-β-d-glucopyranoside, germanaism B, and nigricin were recognized by comparing their UV/MS spectra, chromatographic retention time (tR) with those of standard reference compounds. I. hungarica and I. variegata showed the highest total amount of phenolic compounds. Germanaism B was the most abundant component in the rhizomes of I. variegata (7.089 ± 0.032 mg/g) and I. hungarica (6.285 ± 0.030 mg/g). The compound analyses showed good calibration curve linearity (r2 > 0.999) and low detection and quantifications limit. These results validated the method for its use in the simultaneous quantitative evaluation of phenolic compounds in the studied Iris sp. I. hungarica and I. variegata rhizomes exhibited antioxidant activity, as demonstrated by the HPLC-ABTS system and NRF2 expression assay and anti-inflammatory activity on respiratory burst in human neutrophils. Moreover, the extracts showed anti-allergic and cytotoxic effects against cancer cells. Anti-coronavirus 229E and lipid formation activities were also evaluated. In summary, potent antioxidant marker compounds were identified in the examined Iris sp.

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Comparison of NSAIDs activity in COX-2 expressing and non-expressing 2D and 3D pancreatic cancer cell cultures

Cited by 35 publications

References 33 publications

Production Optimization and In Vitro Evaluation of Anti-Proliferative, Anti-Oxidant and Anti-Inflammatory Potential of the Antibacterial Peptide MAFP9

Production Optimization and In Vitro Evaluation of Anti-Proliferative, Anti-Oxidant and Anti-Inflammatory Potential of the Antibacterial Peptide MAFP9

Designing Metallogelators Derived from NSAID‐based Zn(II) Coordination Complexes for Drug‐Delivery Applications

Qualitative and Quantitative Analysis of Ukrainian Iris Species: A Fresh Look on Their Antioxidant Content and Biological Activities

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