Comparison of nuclear P16 immunostaining in atypical and normal endocervical glands: A descriptive analytical study

Abstract

Cervical cancer is one of the most common malignancies worldwide and a major problem in the healthcare system. Nowadays, the importance of biomarkers in the diagnosis of malignancies is proven. Some studies have pointed to the role of p16 in the diagnosis of cervical cancer. The purpose of this study was to compare the nucleic reactivity with p16 antibody in atypical versus normal endocervical glands. In this descriptive-analytical study, we enrolled 60 patients who had undergone hysterectomy due to non-endocervical causes at Alzahra teaching hospital of Tabriz University of Medical Sciences, Tabriz, Iran. We selected 25 patients with atypical endocervical glands and 35 subjects with normal glands based on the pathologic examination using hematoxylin and eosin (H&E) staining. Then, we assessed the frequency of nucleic reactivity of the tissues with p16 antibody in both groups. No p16 expression was observed in any of the samples from normal subjects. However, only 2 (8.0%) out of 25 samples from the atypical group, were not reactive to the p16 antibody. In addition, 20 samples (80.0%) were diffusely stained continuous, whereas three samples (12.0%) were stained locally. Accordingly, in the group with atypical endocervical glands, the reaction with p16 antibody was significantly higher than that of normal endocervical glands (P = 0.001). P16 biomarker may play a role in the pathogenesis and progression of cervical cancer and can be used as a diagnostic marker for this purpose.

Expression of P16 biomarker in squamous cell carcinoma of uterine cervix and its association with clinico-pathological parameters: A cross-sectional study

“…Cervical cancer is the third most common cancer in women worldwide with a global prevalence of 11.7%, and accounts as the 5 th most common cause of cancerrelated deaths. Its annual estimated global incidence is 500,000 cases, with India accounting for approximately 100,000 cases [1][2][3] . Cervical cancer is the second common cancer in underdeveloped countries among females 1 .…”

Clinicopathological correlation of invasive squamous cell carcinoma of uterine cervix: A cross-sectional study

“…Pap test for screening cervical cancer has tremendously decreased the incidence of cervical cancer. However, in developing countries, despite the existence of a screening procedure, the women present at the late stage of the disease with high mortality [1][2][3][4] .…”

“…The risk factors are early marriage, early age of first sexual intercourse, early age of first pregnancy, multiple pregnancies, increased parity, use of oral contraceptive pills, multiple sexual partners, low socioeconomic status with nutritional/vitamin deficiencies, poor genital hygiene especially penile hygiene of a male partner, rural women, African-American race, tobacco smoking, sexually transmitted disease, Human Papilloma virus infection, Herpes Simplex virus infection, co-infection with HIV, immune compromised status especially in renal transplanted cases and genetic susceptibility [2][3][4][5][6][7] . In addition, lack of awareness of screening programmes, geographical inaccessibility, lack of an oncologist, negligence of initial symptoms along with financial constraints is responsible for a late presentation and diagnosis [7][8][9] .…”

Immunohistochemical expression of p16INK4a in premalignant lesions and malignant tumours of cervix

“…With India accounting for approximately 1,00,000 cases. [1][2][3] Cervical cancer is the second most common cancer in underdeveloped countries. 1 The age range for cervical cancer is reported to be 27-80 years with mean age of 54.2 years; the maximum cases have been noted between 41-60 years of age.…”