Comparison of nucleolar organiser regions and DNA flow cytometry in the evaluation of pleural effusion.

Abstract

AgNOR method, which is rapid and easy to perform, may be a useful adjunct to flow cytometry, S phase fraction analysis and conventional cytology in the routine diagnosis of malignant pleural effusion.

Silver-Stained Nucleolar Organizer Regions in Normal and Dysplastic Cervical Lesions: Correlation with DNA Ploidy and S-Phase Fraction by Flow Cytometry

“…Indeed, 87% of HSIL were positive for high AgNOR counts and, interestingly, 85% for high SPF, which confirms that they could be useful proliferation markers (table 2). These results are also in accordance with previous studies conducted elsewhere [29, 30], which found that AgNOR quantitation reflects changes in DNA ploidy as well as cell proliferation. Moreover, in this study, we have also found a correlation between the increase in SPF and AgNOR counts in the ASCUS and LSIL groups (table 2).…”

“…Several studies also reported that aneuploid DI and high SPF significantly correlated with patient survival, representing a subgroup with a poor prognosis in cancer cases [30, 39, 40]. The survival of patients who had SPF >12% was significantly worse than that of those who had SPF ≤12% in cervical cancer cases [39].…”

Image Analysis and Flow Cytometric DNA Studies of Benign and Malignant Body Cavity Fluids: Reappraisal of the Role of Current Methods in the Differential Diagnosis of Reactive Versus Malignant Conditions

“…Mesothelial cells infrequently have a high S-phase fraction in reactive conditions (up to 16% by image analysis), with less variance from the average (5.4%). Similar results have been reported previously (26).…”

Diagnostic Value of p53 Protein and Flow Cytometric DNA Analysis in the Study of Serous Effusions

“…FCM DNA analysis has been shown to be useful for the detection of malignant cells in a variety of serous effusions. [13][14][15]23,24,27,28,32 The criteria for the determination of aneuploidy differs from author to author. We consider as aneuploidy only those cases with a clearly separate extra peak separable from the diploid cell population.…”

“…This is similar to that reported in other effusion studies. [13][14][15]23,24,27,28,32 In all these studies, the major dilemma in applying FCM to detecting malignant cells is the premise that an absence of aneuploid cells indicates absence of malignancy. All tumors can contain diploid-only cells, 4,10 and aneuploidy is not always synonymous with malignancy.…”