Comparison of nylon-flocked swab and cellulose sponge methods for carbapenem-resistant <i>Enterobacteriaceae</i> and gram-negative organism recovery from high-touch surfaces in patient rooms

Rock, Clare; Anderson, Mary B.; Lewis, Shawna; Scheeler, Verna; Nowakowski, Elaine; Hsu, Yea‐Jen; Milstone, Aaron M.; Carroll, Karen C.; Maragakis, Lisa L.; Simner, Patricia J.; Program, Cdc Prevention Epicenters

doi:10.1017/ice.2018.182

Cited by 7 publications

(7 citation statements)

References 9 publications

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“…The hospital environment can facilitate transmission of pathogens responsible for HAIs. The inanimate environment can be a MDRO reservoir [19,20], with environmental contamination responsible for approximately 10-30% of patient MDRO acquisitions [21]. Table 1 summarizes select key studies on the role of the hospital environment in MDRO and other pathogen transmission.…”

Section: The Hospital Environmentmentioning

confidence: 99%

One Health in hospitals: how understanding the dynamics of people, animals, and the hospital built-environment can be used to better inform interventions for antimicrobial-resistant gram-positive infections

Dalton

Rock

Carroll

et al. 2020

Antimicrob Resist Infect Control

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Despite improvements in hospital infection prevention and control, healthcare associated infections (HAIs) remain a challenge with significant patient morbidity, mortality, and cost for the healthcare system. In this review, we use a One Health framework (human, animal, and environmental health) to explain the epidemiology, demonstrate key knowledge gaps in infection prevention policy, and explore improvements to control Gram-positive pathogens in the healthcare environment. We discuss patient and healthcare worker interactions with the hospital environment that can lead to transmission of the most common Gram-positive hospital pathogens – methicillin-resistant Staphylococcus aureus, Clostridioides (Clostridium) difficile, and vancomycin-resistant Enterococcus – and detail interventions that target these two One Health domains. We discuss the role of animals in the healthcare settings, knowledge gaps regarding their role in pathogen transmission, and the absence of infection risk mitigation strategies targeting animals. We advocate for novel infection prevention and control programs, founded on the pillars of One Health, to reduce Gram-positive hospital-associated pathogen transmission.

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Section: The Hospital Environmentmentioning

confidence: 99%

One Health in hospitals: how understanding the dynamics of people, animals, and the hospital built-environment can be used to better inform interventions for antimicrobial-resistant gram-positive infections

Dalton

Rock

Carroll

et al. 2020

Antimicrob Resist Infect Control

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“…der Sensitivität waren Nylon-Flockfaser-Abstrichtupfer den Celluloseschwämmchen zum Nachweis Gram-negativer Bakterien überlegen [356] bzw. bei CRE gleichwertig [357]. Für den Nachweis Gram-nega-tiver Bakterien sind Abstrichtupfer Kontaktplatten überlegen, für Gram-positive Kokken ist es umgekehrt [358]…”

Section: Durchführung Des Monitoringsunclassified

Anforderungen an die Hygiene bei der Reinigung und Desinfektion von Flächen

2022

Bundesgesundheitsbl

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“…Choice of transport medium is important, and the choice should vary depending on the target organism, time taken to transport to the laboratory, and post-test storage conditions and storage time [29,30]. Neutralizing broths help to keep microbial cells intact while also neutralizing any chemical cleaning substances that may have been collected along with the microbiological sample [31,32]. Some transport media allow inhibition of growth to enable more accurate estimation of counts [29].…”

Section: Transport Media and Neutralizersmentioning

confidence: 99%

“…This method is able to identify a range of bacteria, mycobacteria and fungi by looking at their protein fingerprint, based on the charge and size of the proteins. A number of the studies included in this review used MALDI-TOF to confirm species identification after using selective media and phenotypic tests [7,31,43].…”

Section: Molecular Biology Processingmentioning

confidence: 99%

How to carry out microbiological sampling of healthcare environment surfaces? A review of current evidence

Rawlinson

Ciric

Cloutman-Green

2019

Journal of Hospital Infection

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There is increasing evidence that the hospital surface environment contributes to the spread of pathogens. However, evidence on how best to sample these surfaces is inconsistent and there is no guidance or legislation in place on how to do this. The aim of this review was to assess current literature on surface sampling methodologies, including the devices used, processing methods, and the environmental and biological factors that might influence results. Studies published prior to March 2019 were selected using relevant keywords from ScienceDirect, Web of Science, and PubMed. Abstracts were reviewed and all data-based studies in peer-reviewed journals in the English language were included. Microbiological air and water sampling in the hospital environment were not included. Although the numbers of cells or virions recovered from hospital surface environments were generally low, the majority of surfaces sampled were microbiologically contaminated. Of the organisms detected, multidrug-resistant organisms and clinically significant pathogens were frequently isolated and could, therefore, present a risk to vulnerable patients. Great variation was found between methods and the available data were incomplete and incomparable. Available literature on sampling methods demonstrated deficits with potential improvements for future research. Many of the studies included in the review were laboratory-based and not undertaken in the real hospital environment where sampling recoveries could be affected by the many variables present in a clinical environment. It was therefore difficult to draw overall conclusions; however, some recommendations for the design of routine protocols for surface sampling of healthcare environments can be made.

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Comparison of nylon-flocked swab and cellulose sponge methods for carbapenem-resistant Enterobacteriaceae and gram-negative organism recovery from high-touch surfaces in patient rooms

Cited by 7 publications

References 9 publications

One Health in hospitals: how understanding the dynamics of people, animals, and the hospital built-environment can be used to better inform interventions for antimicrobial-resistant gram-positive infections

One Health in hospitals: how understanding the dynamics of people, animals, and the hospital built-environment can be used to better inform interventions for antimicrobial-resistant gram-positive infections

Anforderungen an die Hygiene bei der Reinigung und Desinfektion von Flächen

How to carry out microbiological sampling of healthcare environment surfaces? A review of current evidence

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