Comparison of Oligosaccharides in Milk Specimens from Humans and Twelve Other Species

Warren, Christopher D.; Chaturvedi, Prasoon; Newburg, Adrienne; Oftedal, Olav T.; Tilden, Christopher D.; Newburg, David S.

doi:10.1007/978-1-4615-1371-1_40

Cited by 45 publications

(45 citation statements)

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“…Levels in ruminant milks are low [23], whereas primate milks typically contain more and more diverse oligosaccharides [24], as do elephants [25]. Nonetheless, there does not seem to be any clear indication of their functional importance that can be deduced from phylogenetic comparisons.…”

Section: Discussionmentioning

confidence: 99%

Isolation of Milk Oligosaccharides using Solid-Phase Extraction

Ward¹

2009

TOGLYJ

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Abstract:A method was developed to isolate mono-and disaccharide-free oligosaccharides from human and bovine milk using a combination of enzymatic digestion of lactose and solid-phase extraction. In the initial trial, 2.5 g of oligosaccharides were isolated from one liter of human milk. In subsequent trials this was increased to over 5 g of oligosaccharides per liter. Compared to filtration-based extraction methods, this procedure allows for further isolation of oligosaccharide fractions via modulation of the column rinsing solvent. Neutral monosaccharide composition of the oligosaccharide polymers was investigated using gas chromatographic analysis of the monosaccharides as alditol acetate derivatives. Results indicate oligosaccharides are approximately made up of 24% fucose, 41% galactose, 22% glucose and 13% glucosamine. Isolated bovine and human milk oligosaccharides were compared to lactose as fermentation substrates for Bifidobacterium longum biovar infantis. Lactose fermentation yielded the greatest production of biomass followed by bovine and human milk oligosaccharides.

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Section: Discussionmentioning

confidence: 99%

Isolation of Milk Oligosaccharides using Solid-Phase Extraction

Ward¹

2009

TOGLYJ

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Section: Introductory Statementmentioning

confidence: 99%

“…Several methods for the separation and quantification of neutral compounds in human milk have been described, including high-performance liquid chromatography (HPLC) [12][13][14][15] and capillary electrophoresis (CE) of derivatized HMOS [16], and HPLC with high pH amperometric detection of underivatized HMOS [17]. Acidic oligosaccharides have been resolved by anion exchange HPLC of radiolabelled derivatives [18], derivatization of the oligosaccharides followed by ion exchange LC with absorption at 200 nm [19] and capillary electrophoresis of fluorescent derivatives [20,21].…”

Section: Introductory Statementmentioning

confidence: 99%

“…Human milk oligosaccharides (HMOS), a principal component of human milk, are found in concentrations of 6 to 12 g/L, and these oligosaccharides vary in size, structure, and abundance [1]. The oligosaccharides of milks from other species vary widely, and the composition of oligosaccharides from human milk is unique [2].HMOS play a potentially protective role to nursing infants. Breastfed infants have a different microbiota than infants fed artificially, and human milk glycans are thought to act as prebiotics that promote the growth of Bifidobacterium bifidus [3].…”

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Simultaneous quantification of sialyloligosaccharides from human milk by capillary electrophoresis

Bao

Zhu

Newburg

2007

Analytical Biochemistry

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The acidic oligosaccharides of human milk are predominantly sialyloligosaccharides. Pathogens that bind sialic acid-containing glycans on their host mucosal surfaces may be inhibited by human milk sialyloligosaccharides, but testing this hypothesis requires their reliable quantification in milk. Sialyloligosaccharides have been quantified by anion exchange HPLC, reverse or normal phase HPLC, and capillary electrophoresis (CE) of fluorescent derivatives; in milk, these oligosaccharides have been analyzed by high pH anion exchange chromatography with pulsed amperometric detection, and, in our laboratory, by CE with detection at 205 nm. The novel method described herein uses a running buffer of aqueous 200 mM NaH 2 PO 4 at pH 7.05 containing 100 mM SDS made 45% (v/v) with methanol to baseline resolve five oligosaccharides, and separate all 12. This allows automated simultaneous quantification of the 12 major sialyloligosaccharides of human milk in a single 35-minute run. This method revealed differences in sialyloligosaccharide concentrations between less and more mature milk from the same donors. Individual donors also varied in expression of sialyloligosaccharides in their milk. Thus, the facile quantification of sialyloligosaccharides by this method is suitable for measuring variation in expression of specific sialyloligosaccharides in milk and their relationship to decreased risk of specific diseases in infants. Keywordshuman milk oligosaccharides; sialyloligosaccharides; capillary electrophoresis INTRODUCTORY STATEMENTHuman milk contains a large number of compounds that provide nutritional support and contribute toward defense of the newborn. Human milk oligosaccharides (HMOS), a principal component of human milk, are found in concentrations of 6 to 12 g/L, and these oligosaccharides vary in size, structure, and abundance [1]. The oligosaccharides of milks from other species vary widely, and the composition of oligosaccharides from human milk is unique [2].HMOS play a potentially protective role to nursing infants. Breastfed infants have a different microbiota than infants fed artificially, and human milk glycans are thought to act as prebiotics that promote the growth of Bifidobacterium bifidus [3]. Breast-fed infants have fewer or less severe gastrointestinal and respiratory infections during the first year of life than formula-fed

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“…Derivatives of neutral oligosaccharides were separated on normal phase columns [14] or RP columns with UV detection [15][16][17][18]. Underivatized neutral or acidic oligosaccharides have been resolved by high-pH anion-exchange chromatography and detected by pulsed amperometry [19][20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis of acidic oligosaccharides from human milk

Bao

Newburg

2008

Electrophoresis

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Interest in defining the array of oligosaccharides of human milk has been increasing. Pathogens that bind glycans on their host mucosal surfaces may be inhibited by human milk oligosaccharides. It has been postulated that acidic oligosaccharides in human milk may inhibit binding by pathogens that bind acidic glycans in the gut, but testing this hypothesis requires their reliable quantification in milk. Sialyloligosaccharides of human milk have been quantified by HPLC and CE. A recent CE technique uses the MEKC mode with direct detection at 205 nm to resolve and quantify, in the native form, the 12 most dominant sialyloligosaccharides of human milk in a single 35-min run. The method gives a linear response from 39 to 2500 microg/mL with a coefficient of variation between 2 to 9% and accuracy from 93 to 109%. This was used to detect variation in expression of specific sialyloligosaccharides in milk. Individual sialyloligosaccharide concentrations in milk differ among individual donors and between less and more mature milk. Thus, CE can be used to measure variation in sialyloligosaccharide expression in milk, and thereby test the relationship of this variation-to-variation in risk of specific diseases in breastfed infants.

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Comparison of Oligosaccharides in Milk Specimens from Humans and Twelve Other Species

Cited by 45 publications

References 6 publications

Isolation of Milk Oligosaccharides using Solid-Phase Extraction

Isolation of Milk Oligosaccharides using Solid-Phase Extraction

Simultaneous quantification of sialyloligosaccharides from human milk by capillary electrophoresis

Capillary electrophoresis of acidic oligosaccharides from human milk

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