Comparison of ortho respiratory syncytial virus enzyme-linked immunosorbent assay and HEp-2 cell culture

Bromberg, Kenneth; Tannis, G; Daidone, B; Clarke, Lorraine; Sierra, Marielys Figueroa

doi:10.1128/jcm.22.6.1071-1072.1985

Cited by 32 publications

(13 citation statements)

References 7 publications

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“…In other RSV studies 10 to 20% of the (5,11,12,15,37,44,46,49,52). The reported sensitivity of enzyme-linked immunosorbent assay and radioimmunoassays when compared with culture or immunofluorescence methods have ranged from 61 to 96% (1,7,8,10,12,14,16,18,28,29,35,37,39,44,48,49,53).…”

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Detection of respiratory syncytial virus in clinical specimens by viral culture, direct and indirect immunofluorescence, and enzyme immunoassay

1988

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We evaluated prospectively the detection of respiratory syncytial virus (RSV) by culture and by direct antigen detection using an indirect immunofluorescence assay (IFA), a direct monoclonal immunofluorescence assay (DFA), and a monoclonal enzyme immunoassay (EIA). Of 221 specimens, 95 (43%) were culture positive for RSV, 4 (1.8%) contained more than one virus, and 17 (7.6%) contained a virus other than RSV. Overall, HEp-2 and Flow 6000 cells grew significantly more RSV isolates (82 and 72%, respectively) than A549 cells, which grew only 29% of the isolates. The mean time for RSV detection with HEp-2 cells was 2.9 days. This was significantly less than the mean time for RSV detection with either Flow 6000 cells (6.1 days) or A549 cells (6.4 days). Of 221 specimens, 129 were tested simultaneously by culture, IFA, and DFA. Of these 129 specimens, 62 (48%) were positive by culture, 69 (53%) were positive by IFA, and 70 (54%) were positive by DFA. For 92 specimens screened simultaneously by culture, IFA, and EIA, positive results were obtained for 33 (36%) of the specimens by both culture and IFA and for 29 (32%) of the specimens by EIA. Of 126 culture-negative specimens, 21 (17%) were positive for RSV when determined by IFA. Conversely, 14 (15%) of 95 RSV culture-positive specimens were negative by IFA, whereas DFA missed 19% of the culture-positive specimens. Compared with culture, the Kallestad EIA kit had a sensitivity and specificity of 73 and 92% respectively, but missed 9 (27%) of 33 culture-positive specimens. These data demonstrate that isolation by culture continues to be important for viral diagnosis of RSV infections and that for valid comparative studies between viral isolations and rapid detection methods, both sensitive host cells and appropriate test conditions are required.

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confidence: 99%

Detection of respiratory syncytial virus in clinical specimens by viral culture, direct and indirect immunofluorescence, and enzyme immunoassay

1988

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“…Immunofluorescence (IF) and enzyme-linked immunosorbent assay (ELISA) tests for the detection of respiratory viruses have been widely used [Bromberg et al, 1985;Chonmaitree et al, 1987;Hendry et al, 1982;Hornsleth and Friis, 1982;Kaul et al, 19781 and found to be useful diagnostic techniques [Grandien et al, 1985;Harmon and Pawlik, 19821, both affording speed and accuracy.…”

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Comparison of three techniques for detection of respiratory viruses in nasopharyngeal aspirates from children with lower acute respiratory infections

Salomón

Grandien

Ávila

et al. 1989

Journal of Medical Virology

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A comparison of immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), and isolation in tissue culture (TC) for detection of respiratory viruses was performed on 496 nasopharyngeal aspirates from children under 5 years of age with lower acute respiratory infections who were receiving attention at three hospitals in Buenos Aires, Argentina. All samples were tested by the three methods for respiratory syncytial virus (RSV), influenza A and B, adenovirus, and parainfluenza 1 and 3. Viral diagnosis was made in 167 samples (33.7%); of these, 124 (74.3%) were isolated in TC, whereas 120 (71.8%) were detected by ELISA and 127 (76%) by IF. RSV was detected in 121 samples, mainly by ELISA and IF. The sensitivity and specificity of each rapid technique as compared with isolation in TC were similar, reaching 98% and 92%, respectively. When ELISA was compared with IF, the sensitivity was 95%, and the specificity was 98%. Adenovirus was detected in 18 patients by TC. For this virus, rapid techniques sensitivity as compared with TC was low (almost 22%). Parainfluenza 3 was readily detected by IF and TC; influenza A, B and parainfluenza 1 were detected in few samples; and tissue culture proved more efficient than rapid techniques. The results indicate that both rapid techniques are good tools for the detection of most respiratory viruses except for adenovirus, for which TC cannot be omitted.

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“…Confirmation of a viral etiology for respiratory infections is important, particularly as more antiviral therapy becomes available (11,13). The success of detection of a respiratory virus depends on many variables, including the site of collection and the type of specimen used (1,4,6,8,10,12,(14)(15)(16)(17)(18)20). The most commonly recommended respiratory specimens for viral isolation in children are nasal washes and nasopharyngeal aspirates (NPAs) (1,8,12,17,18,20).…”

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“…In the remaining 33 patients, the order of collection was not noted. NPA specimens were collected by suction of nasopharyngeal secretions from one nostril into mucous traps, as described previously (1,4,8). Secretions remaining in the catheter were washed off by suctioning 1 ml of viral transport medium (minimal essential medium containing 25 mM N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid [HEPES; pH 7.4], 5.4 mM sodium bicarbonate, 10 ,ug of gentamicin per ml, and 0.6% fetal bovine serum) through the catheter.…”

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confidence: 99%

“…In seven instances, the culture of one or both specimens was contaminated and therefore was excluded from the analysis, leaving 119 paired specimens for comparison. There were 46 instances in which both NPS and NPA specimens yielded virus in cell culture, 4 instances in which only the NPS specimens were positive, and 9 instances in which only the NPA specimens were positive. The difference in the isolation rates from the NPS (42.0%) and NPA (46.2%) specimens was not statistically significant (P > 0.10).…”

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Nasopharyngeal swabs and nasopharyngeal aspirates equally effective for the diagnosis of viral respiratory disease in hospitalized children

et al. 1989

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Comparison of ortho respiratory syncytial virus enzyme-linked immunosorbent assay and HEp-2 cell culture

Abstract: Two hundred seventy nasopharyngeal aspirates were tested in duplicate with the Ortho Diagnostics, Inc. (Raritan, N.J.), respiratory syncytial virus antigen enzyme-linked immnunosorbent assay. The test was sensitive

Cited by 32 publications

References 7 publications

Detection of respiratory syncytial virus in clinical specimens by viral culture, direct and indirect immunofluorescence, and enzyme immunoassay

Detection of respiratory syncytial virus in clinical specimens by viral culture, direct and indirect immunofluorescence, and enzyme immunoassay

Comparison of three techniques for detection of respiratory viruses in nasopharyngeal aspirates from children with lower acute respiratory infections

Nasopharyngeal swabs and nasopharyngeal aspirates equally effective for the diagnosis of viral respiratory disease in hospitalized children

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