Comparison of oxidative stress induced by clarithromycin in two freshwater microalgae Raphidocelis subcapitata and Chlorella vulgaris

Guo, Jiahua; Jie, Peng; Yuan, Lei; Kanerva, Mirella; Li, Qi; Song, Jinxi; Guo, Jipu; Sun, Haotian

doi:10.1016/j.aquatox.2019.105376

Cited by 62 publications

(29 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the contrary, in N. gaditana a significant change was observed in terms of the abundance of violaxanthin, where the increase in production under continuous mode reached 10% and 40% under the pulse exposure mode. This outcome indicates that the production of ROS occurs as a consequence of the oxidative stress in cells caused by the exposure to SMF, coinciding with Guo et al and Lozano et al [ 40 , 41 ], where the ROS formation increases the enzymatic activity with respect to the control. In addition, in the future it will be interesting to use pulse SMF exposure as an alternative to increase violaxanthin production as other stress conditions such as temperature, salinity, and irradiance may induce carotenoid production in algal species [ 42 ].…”

Section: Discussionsupporting

confidence: 80%

Response to Static Magnetic Field-Induced Stress in Scenedesmus obliquus and Nannochloropsis gaditana

et al. 2021

View full text Add to dashboard Cite

Magnetic fields in biological systems is a promising research field; however, their application for microalgae has not been fully exploited. This work aims to measure the enzymatic activity and non-enzymatic activity of two microalgae species in terms of superoxide dismutase (SOD), catalase (CAT), and carotenoids, respectively, in response to static magnetic fields-induced stress. Two magnet configurations (north and south) and two exposure modes (continuous and pulse) were applied. Two microalgae species were considered, the Scenedesmus obliquus and Nannochloropsis gaditana. The SOD activity increased by up to 60% in S. obliquus under continuous exposure. This trend was also found for CAT in the continuous mode. Conversely, under the pulse mode, its response was hampered as the SOD and CAT were reduced. For N. gaditana, SOD increased by up to 62% with the south configuration under continuous exposure. In terms of CAT, there was a higher activity of up to 19%. Under the pulsed exposure, SOD activity was up to 115%. The CAT in this microalga was increased by up to 29%. For N. gaditana, a significant increase of over 40% in violaxanthin production was obtained compared to the control, when the microalgae were exposed to SMF as a pulse. Depending on the exposure mode and species, this methodology can be used to produce oxidative stress and obtain an inhibitory or enhanced response in addition to the significant increase in the production of antioxidant pigments.

show abstract

Section: Discussionsupporting

confidence: 80%

Response to Static Magnetic Field-Induced Stress in Scenedesmus obliquus and Nannochloropsis gaditana

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Superoxide dismutase (SOD) is a key enzyme in the antioxidant system, which functions as a pioneer in defending against ROS to protect organisms [59]. Based on this, it is demonstrated that the enhanced of SOD after 80 µg L −1 Clarithromycin exposure in algae Chlorella vulgaris [19]. Previous studies also reported that elevated SOD activities attenuate the oxidative damage caused by several pollution exposures (e.g.…”

Section: Genes Involved In Antioxidant Defensementioning

confidence: 98%

“…Moreover, roxithromycin (ROX) can inhibit fatty acid synthesis to refrain the algae growth by Fourier transform infrared spectroscopy [16]. Extra ROS is likely to produce in algal cells at antibiotics exposure (e.g., roxithromycin and clarithromycin) [17][18][19]. However, the toxic molecular mechanism in green algae R. subcapitata remains largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Lincomycin-Induced Transcriptional Alterations in the Green Alga Raphidocelis subcapitata

et al. 2020

Self Cite

View full text Add to dashboard Cite

Lincomycin (LIN), as a waterborne contaminant, may pose a threat to algal health and may affect the provision of ecosystem services. In addition, the molecular mechanisms of lincomycin in algae are still unknown. Here, we attempted to use the transcriptome analysis to elucidate for the first time the potential impact of LIN at an environmentally relevant concentration on the algal growth, and verify the hypothesis that lincomycin can disrupt algal protein synthesis by combining with its subunits of ribosome at high-LIN level. In this study, 7-day growth inhibition tests and RNA-seq sequencing were conducted in Raphidocelis subcapitata (R. subcapitata) in response to a LIN at the concentrations of 0.5 µg L−1 (low), 5 µg L−1 (medium), and 400 µg L−1 (high) treatment groups. A negligible influence on algal growth and merely 21 (21 up- and 0 downregulated) differentially expressed genes (DEGs) was observed at low concentration of LIN, and medium groups showed a 13.4% inhibition and 92 (64 up- and 48 downregulated) DEGs, while high-LIN dosing caused 65.4% reduction in algal growth and 2514 (663 up- and 1851 downregulated) DEGs. In 0.5 and 5 µg L−1 groups, LIN upregulated the genes in the process of photosynthesis consisting of photosynthesis-antenna proteins, and porphyrin and chlorophyll metabolism pathways, suggesting that photosynthesis at low LIN exposure was more sensitive than algal growth. Whereas DEGs in the 400 µg L−1 group were mostly enriched in carbohydrate, carbon fixation in photosynthetic organisms, and nucleotide metabolism pathways. Furthermore, genes involved in detoxification processes were nearly downregulated in high-LIN group. In addition, genes encoding the antioxidant enzymes in the peroxisome pathway such as superoxide dismutase (sod2), peroxin-2 (pex2), 2,4-dienoyl-CoA reductase ((3E)-enoyl-CoA-producing) (decr2) were upregulated, which are responsible for deleting extra intracellular reactive oxygen species (ROS) caused by LIN to protect algal health, suggesting the occurrence of oxidative stress. Taken together, this is the first meticulous study unraveling the molecular mechanism of antibiotics in algae.

show abstract

“…The formation of malondialdehyde (MDA) as an FA peroxidation product characterizes pathological processes in a living cell. MDA can appear in water organisms under the impact of anionic surfactants, such as linear alkylbenzene sulphonates (LASs) [41,42,54], heavy metals [55], polycyclic aromatic hydrocarbons (PAH) [56], and antibiotics [57]. According to a previous investigation [58], anionic surfactants found in the The difference between the phytoplankton from the central pelagic stations and the offshore pelagic stations is possibly related to the distance from the shore.…”

Section: Fa Composition and Fa Peroxidation Product Analysismentioning

confidence: 99%

Fatty Acid Changes in Nearshore Phytoplankton under Anthropogenic Impact as a Biodiversity Risk Factor for the World’s Deepest Lake Baikal

et al. 2022

View full text Add to dashboard Cite

In this study, we present results on fatty acid analysis of phytoplankton of Lake Baikal, the world’s deepest lake, which differs from other lakes by its oceanic features. Since we used a large-mesh net, the net sample phytoplankton were primarily represented by the large elongated diatom Synedra acus. subsp. radians (Kützing) Skabichevskij. The similar algae composition of net samples of spring season phytoplankton collected at different sites of the lake allows us to compare results of the fatty acid analysis of these samples. The phytoplankton diversity of the sedimentation samples was contrary represented by 32 algae species. There are clear changes in the fatty acid composition of net phytoplankton exposed to anthropogenic impacts of varying intensity. The content of polyunsaturated fatty acids in phytoplankton collected from central stations (pelagic stations at a distance of ~10–30 km from the shoreline) without anthropogenic impact was higher by up to 15% than phytoplankton collected from nearshore stations (littoral stations at a distance of ~0.01–0.05 km from the shoreline) and offshore stations (pelagic stations at a distance of ~3 km from the shoreline). The interlaboratory precision of fatty acid determination of phytoplankton is estimated as ≤10%. We found high content of the lipid peroxidation marker (80–340 μg g−1 of dry weight) in phytoplankton from nearshore and offshore stations with intensive anthropogenic impact. In phytoplankton from central stations, we did not find any lipid peroxidation. Determination of unsaturated fatty acids, coupled with analysis of fatty acid peroxidation products, can be used to evaluate the level of anthropogenic impact in terms of ecological health and biodiversity conservation.

show abstract

Comparison of oxidative stress induced by clarithromycin in two freshwater microalgae Raphidocelis subcapitata and Chlorella vulgaris

Cited by 62 publications

References 60 publications

Response to Static Magnetic Field-Induced Stress in Scenedesmus obliquus and Nannochloropsis gaditana

Response to Static Magnetic Field-Induced Stress in Scenedesmus obliquus and Nannochloropsis gaditana

Lincomycin-Induced Transcriptional Alterations in the Green Alga Raphidocelis subcapitata

Fatty Acid Changes in Nearshore Phytoplankton under Anthropogenic Impact as a Biodiversity Risk Factor for the World’s Deepest Lake Baikal

Contact Info

Product

Resources

About