Comparison of ozone, hydrogen peroxide and removal of infected eggs for prevention of fungal infection in sturgeon hatchery

The direct exposure of fish eggs to ozonated water has generated interest as a means of ensuring pathogen-free eggs without the use of harsh chemicals. However, there are numerous knowledge gaps, including safe contact times, exposure levels, and potential long-term effects on aquaculture species in both freshwater and seawater. The effect of different ozone (O3) doses (0.5-1.0, 1.5-2.0, and 2.5-3.0 mg of O3/L for 90 s) on recently fertilized eggs of Atlantic Cod Gadus morhua and eyed eggs of Atlantic Salmon Salmo salar and Rainbow Trout Oncorhynchus mykiss was evaluated in comparison with the effects of two commercial disinfectants: Perosan (0.004 mg/L) and Ovadine (100 mg/L). The impact of ozone application was evaluated based on hatching success, larval nucleic acid concentration, larval growth, and survival. Overall, results indicated that ozonation of Atlantic Cod eggs at a dose less than 3.0 mg/L for 90 s produced no negative effect on the larvae up to 30 d posthatch. Furthermore, ozonation of Atlantic Salmon and Rainbow Trout eggs generated no negative effect on the larvae, based on monitoring until 85% yolk sac re-absorption (16 d posthatch).

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The Impact of Egg Ozonation on Hatching Success, Larval Growth, and Survival of Atlantic Cod, Atlantic Salmon, and Rainbow Trout

Fry

Casanova

Hamoutene

et al. 2015

J Aqua Anim Hlth

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Effects of different levels of ozone on ammonia, nitrite, nitrate, and dissolved organic carbon in sterilization of seawater

Rahmadi

Kim

2014

Desalination and Water Treatment

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Ozone is applied to the recirculation aquaculture system to reduce bacteria and parasites. Besides the sterilization effects, it is known that ozone has oxidizing effects on some water quality parameters. Therefore, oxidizing effects of ozone on ammonia (NH 4 -N), nitrite (NO 2 -N), nitrate (NO 3 -N), and dissolved organic carbon were tested in this study. During the test, ozone effects on pH, dissolved oxygen (DO) and bromination were also monitored. Ozone concentrations were originally set to 0.05, 0.1, 0.15, 0.2, and 0.25 ppm, but actual treatment concentrations were maintained at 0.04, 0.11, 0.15, 0.19, and 0.23 ppm. The 5 ppm of NH 4 -N was oxidized within 12 h in all concentrations of ozone treatments, with the average oxidizing rate of 0.65 ± 0.28 mg NH 4 -N/L per h. The 5 ppm of NO 2 -N was oxidized within 1.5 h in all concentrations of ozone treatments at a rate of 4.5 mg NO 2 -N/L per h. One of 5 ppm NO 3 -N was oxidized by all concentration of ozone treatment after 24 h. In addition, ozone also oxidized dissolved organic carbon and maintained the concentration at about 2.9 ± 0.77 ppm from the 15 ppm of initial concentration by 12 h. DO was increased from 5.9 to 9.4 ppm within 30 min in all ozone treatment and stabilized thereafter. Bromate concentrations increased sharply within the first 6 h of ozonation at the rate of 7.3 ± 2.4 mg/L per h in almost all ozone treatments; the rate decreased to 2.5 ± 0.15 mg/L per h thereafter. However, bromate concentration was not increased in the ammonia experiment until all ammonia was oxidized. Therefore, further studies are needed to determine the relationship between NH 4 -N concentration and bromate formation in seawater.

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Direct application of ozone in aquaculture systems

Powell

Scolding

2016

Reviews in Aquaculture

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Ozone (O3) is a powerful oxidant that has been used in both the aquaculture and water treatment industries to improve water quality and reduce pathogens during pretreatment, treatment of effluent, as a continual treatment during RAS operations, and for bivalve depuration. As ozone can be toxic to aquatic organisms, the technology has also been investigated to destroy invasive or nuisance species, and other research has also highlighted negative effects of residual ozone on water courses. Ozone and ozone‐produced oxidants used in aquaculture operations have therefore typically been removed from water prior to entry into tanks holding stock animals. However, a growing body of research has identified direct application of ozone, here defined as exposure of residual ozone and ozone‐produced oxidants to cultured species of finfish, shellfish and live feeds across various life stages. This approach appears to be increasingly employed as a beneficial technology due to proven enhancement of hygiene and water quality, provided dosages or concentrations are appropriate to maintain animal health and welfare. This review paper concentrates on the observed benefits and drawbacks of direct ozonation, influencing factors and future considerations for standardisation and uptake of the technology.

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Comparison of ozone, hydrogen peroxide and removal of infected eggs for prevention of fungal infection in sturgeon hatchery

Cited by 3 publications

References 18 publications

The Impact of Egg Ozonation on Hatching Success, Larval Growth, and Survival of Atlantic Cod, Atlantic Salmon, and Rainbow Trout

The Impact of Egg Ozonation on Hatching Success, Larval Growth, and Survival of Atlantic Cod, Atlantic Salmon, and Rainbow Trout

Effects of different levels of ozone on ammonia, nitrite, nitrate, and dissolved organic carbon in sterilization of seawater

Direct application of ozone in aquaculture systems

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