Comparison of Pain-Like behaviors in two surgical incision animal models in C57BL/6J mice

Genome-wide association studies with experimental validation identify a protective role for B lymphocytes against chronic post-surgical pain

“…Based on the results from genetic analyses implicating a contribution of lymphocytes to development of CPSP, we directly tested the role of the adaptive immune system in post-surgical pain using mouse assays. We used plantar incision and laparotomy, two well-characterised preclinical post-surgical pain models, 18 to assess pain outcomes. Although the partitioned heritability analysis did not provide the direction of the observed contribution of the adaptive immune system, we started from the hypothesis that B and T cells were needed for pain resolution in CPSP, testing Rag1 –/– mice which lack mature B and T lymphocytes.…”

Genome-wide association study suggests a critical contribution of the adaptive immune system to chronic post-surgical pain

“…[45] We utilized two well-characterizedpreclinical post-surgical pain assays: plantar incision and laparotomy. [46]…”

“…[45] We utilized two well-characterizedpreclinical post-surgical pain assays: plantar incision and laparotomy. [46] In the plantar incision assay, withdrawal thresholds to evoked mechanical stimulation (using von Frey filaments) aimed at the plantar hind paw before and at multiple time points up to the 84th day after hind-paw incision were measured in C57BL/6 (wildtype) and Rag1 null mutant (Rag1 -/-) mice lacking T-and B-cells. Repeated measures ANOVA revealed significant interactions of repeated measure with strain and sex (F13,221 = 2.1, p=0.015).…”