Comparison of PCR and Conventional Cultural Method for Detection of Salmonella 
 from Poultry Blood and Faeces

Manoj, Jinu; Agarwal, Rajesh; Sailo, Blessa; Wani, M.A.; Kumar, Ashok; Dhama, Kuldeep; Singh, M. K.

doi:10.3923/ajava.2014.690.701

Cited by 10 publications

(5 citation statements)

References 21 publications

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“…A prevalence of 12.28% was documented in Tarai region of Uttarakhand from broilers and the serovars were S. gallinarum, S. enteritidis and S. typhimurium. Jinu et al (2014) reported total Salmonella prevalence rate of 5.88% from chicken in Bareilly, Uttar Pradesh and the major serotypes were S. typhimurium and S. enteritidis. Ahmad et al (2014) studied MIC levels of various antibiotics among Indian isolates of S. typhimurium and found a resistance of 93% and 57% for sulfisoxazole and tetracycline, respectively.…”

Section: Indian Scenariomentioning

confidence: 99%

“…al., 2014). The predominant serovars of Salmonella, having public health importance are mainly S. enteritidis and S. typhimurium ( Jinu et al, 2014). Changes in the environment and poultry raising practices along with increased international trading of poultry and its products made changes in predominance of Salmonella serotypes.…”

Section: Public Health Significance Of Salmonellosismentioning

confidence: 99%

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The Role of Poultry in Food Borne Salmonellosis and its Public Health Importance

Manoj¹

2015

Adv. Anim. Vet. Sci.

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| Salmonella organisms are most frequently isolated bacterial agents of food borne outbreaks. Due to its economic importance and risks to human health, salmonellosis poses significant role in public health. Poultry serves as reservoir of Salmonella and poultry meat and eggs have been implicated in food associated outbreaks of salmonellosis. The presence of Salmonella in poultry makes the food chain unsafe from farm to the table. In this review, we explore various public health aspects of food borne salmonellosis originated from poultry. In India, poultry industry is one of the fastest growing segments and the factors linked to salmonellosis in poultry industry are reviewed. The sources and modes of transmission of this organism and the disease symptoms are briefly explained. Various reports of salmonellosis in poultry, with special emphasis on Indian conditions are also discussed. The emergence of multidrug resistant Salmonella is considered as a major global threat to public health, therefore, the antibiotic resistance in Salmonella serovars associated with poultry is also presented.

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Section: Indian Scenariomentioning

confidence: 99%

Section: Public Health Significance Of Salmonellosismentioning

confidence: 99%

The Role of Poultry in Food Borne Salmonellosis and its Public Health Importance

Manoj¹

2015

Adv. Anim. Vet. Sci.

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“…In this study, bacteria culture and phenotypic and biochemical characterization were used as the basis for identification and confirmation of non-typhoidal Salmonella. Culture and phenotypic and biochemical characterization have been confirmed as very sensitive and specific for the identification of NTS, and they compare favourably with PCR and ELISA [ 2 , 29 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

Risk Factors for Persistent Infection of Non-Typhoidal Salmonella in Poultry Farms, North Central Nigeria

et al. 2022

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Salmonellosis is a bacterial zoonosis causing an array of health conditions. Non-typhoidal salmonellosis (NTS) has a discrete adaptation to certain animals; in poultry, pullorum and fowl typhoid are its primary disease manifestations. The diseases are prevalent in Nigerian poultry and have been well-studied in Nigeria, but less so in North Central Nigeria (NCN). Using field sampling, laboratory methods and a semi-structured questionnaire for 1000 poultry farms in NCN, we explored the incidence and risk factors for the persistence of NTS infection in poultry. Approximately 41.6% of the farms had experienced NTS over the last 18 months. Farm experience of NTS moderately predicted awareness of salmonellosis. Increasing stock in smallholder farms, self-mixing of concentrate on the farm, usage of stream water, pen odour, non-adherence and partial adherence of farms to recommended poultry vaccination against pullorum and fowl typhoid and lack of and non-adherence to biosecurity were identified risk factors that increased the odds of NTS infection in poultry. Antibiotic use practice may have reduced the isolation rate of NTS, yet NTS continues to challenge poultry farms in Nigeria. Identified risk practices must be mitigated intentionally and biosecurity and hygiene must be improved to reduce the burden of NTS.

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“…In this study, bacteria culture and phenotypic and biochemical characterization was used as the basis for identification and confirmation, with additional test using the polymerase chain reaction (PCR) were used to for identification; it has been confirmed that these methods (culture and phenotypic and biochemical characterization) were very sensitive and specific for the identification of NTS and they compare favorably with PCR and ELISA [2,29,30]. We obtained samples from broiler, layers and mixed farms but did not consider the hatcheries and parent/grandparent farms because these latter farms need special permission to access and may have to be considered separately.…”

Section: Discussionmentioning

confidence: 99%

Risk Factors for Persistence Infection of Non-typhoidal Salmonella in Poultry Farms, North-Central Nigeria

Sanni¹,

Onyango²,

Usman³

et al. 2022

Preprint

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Salmonellosis is a bacterial zoonosis with array of health conditions. Non-typhoidal salmonellosis (NTS) have a discrete adaptation to certain animals; in poultry, pullorum and fowl typhoid are its primary disease manifestations. The diseases are prevalent in the Nigerian poultry and have been well studied in Nigeria, but less so in the north central Nigeria (NCN). Using field sampling, laboratory methods and semi structured questionnaire in 1000 poultry farms from NCN, we explore the incidence and risk factors for the persistence of NTS infection in poultry. Approximately 41.6% of the farms have experienced NTS but only 6.3% have current infection with NTS. Farm experience of NTS moderately predicted awareness of salmonellosis. Increasing stock in smallholder farms, self-mixing of concentrate on the farm, usage of stream water, pen odour, non-adherence and partial adherence of farms to recommended poultry vaccination against pullorum and fowl typhoid, and lack of and non-adherence to biosecurity were identified risk factors that increased the odds of NTS infection in poultry. Antibiotic use practice may have reduced the isolation rate of NTS, yet NTS continues to challenge poultry farms in Nigeria. Identified risk practices must be mitigated intentionally and biosecurity and hygiene must improve to reduce the burden of NTS.

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Comparison of PCR and Conventional Cultural Method for Detection of Salmonella from Poultry Blood and Faeces

Cited by 10 publications

References 21 publications

The Role of Poultry in Food Borne Salmonellosis and its Public Health Importance

The Role of Poultry in Food Borne Salmonellosis and its Public Health Importance

Risk Factors for Persistent Infection of Non-Typhoidal Salmonella in Poultry Farms, North Central Nigeria

Risk Factors for Persistence Infection of Non-typhoidal Salmonella in Poultry Farms, North-Central Nigeria

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