Comparison of PCR and other diagnostic techniques for detection of Helicobacter pylori infection in dyspeptic patients

Weiss, Judith B.; Mecca, J; Silva, E da; Gassner, Dieter

doi:10.1128/jcm.32.7.1663-1668.1994

Cited by 70 publications

(24 citation statements)

References 34 publications

(39 reference statements)

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“…The rapid-urease test and histological examinations of biopsy specimens detected H. pylori infection in slightly fewer patients than did culture but they still yielded good specificity, although they gave some false-positive results (two and one, respectively). Overall, our results are in good agreement with other recent data for comparisons of different routine tests for H. pylori in which Giemsa staining of histological sections (26) or the agreement among all the tests was taken as the gold standard (13). In both studies PCR assays detected H. pylori infection in a higher percentage of patients.…”

Section: Discussionsupporting

confidence: 91%

Diagnosis of Helicobacter pylori infection by PCR: comparison with other invasive techniques and detection of cagA gene in gastric biopsy specimens

et al. 1995

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A PCR assay for the detection of Helicobacter pylori in gastric biopsy specimens with specific primers for ureC gene amplification (herein referred to as ureC PCR) was compared with other routine invasive methods (culture, the rapid-urease test, and Giemsa staining of histological sections) with samples from a group of 104 consecutive dyspeptic patients. Bacteria were found in 40 (38.5%), 38 (36.5%), 36 (34.6%), and 35 (33.7%) of the patients by ureC PCR, culture, the rapid-urease test, and Giemsa stain, respectively. Sixty-three patients had negative cultures, negative histological examinations, and negative rapid-urease test results, and 61 of these patients were also negative by ureC PCR. ureC PCR detected H. pylori in two culture-negative patients. In parallel, a PCR-based assay to detect the H. pylori cytotoxin-associated antigen (cagA) gene, a putative virulence gene, was also developed. To assess the likelihood of detection of H. pylori genes directly from gastric biopsy samples and from the corresponding H. pylori isolates, specimens from 31 patients were subjected to PCR with ureC-and cagA-targeting primers. All 31 biopsy specimens and the corresponding H. pylori isolates were positive in the ureC PCR. H. pylori strains that were cagA positive also gave positive cagA PCR fragments with biopsy specimens from the same patients. All ureC PCR-positive patients were examined; biopsy specimens from 10 of 11 (91.7%) duodenal ulcer patients harbored H. pylori cagA-positive strains, whereas 19 of 26 (73%) of those from patients with chronic gastritis only were found to be cagA positive. These findings indicate first that ureC PCR is at least as sensitive as culture for diagnosing H. pylori infection and second that the presence of the H. pylori cagA gene can also be detected directly in biopsy specimens by PCR amplification.

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Section: Discussionsupporting

confidence: 91%

Diagnosis of Helicobacter pylori infection by PCR: comparison with other invasive techniques and detection of cagA gene in gastric biopsy specimens

et al. 1995

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“…Initial evidence of H. pylori presence in environmental water samples has come from PCR amplification of samples obtained from Colombia, where infection rates are over 90% (Handwerker et al 1995). In addition to this a number of PCR assays have been utilized over the years for the detection of H. pylori in water (Engstrand et al 1992;Weiss et al 1994;Hulten et al 1996;Sasaki et al 1999;Benson et al 2004;Gomes & De Martinis 2004;Shahamat et al 2004). In this study real time qPCR was shown to be a specific, sensitive and rapid method to quantify H. pylori in sewage.…”

Section: Transmission Of H Pylori In Watermentioning

confidence: 84%

Transmission of Helicobacter pylori and the role of water and biofilms

Percival

Thomas

2009

Journal of Water and Health

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Documented evidence relating to the survival of Helicobacter pylori outside the gastric niche is extremely limited. To date the primary transmission routes of H. pylori have yet to be confirmed and when this is achieved preventive infection control measures can be implemented to reduce and ultimately prevent human infection from this pathogen. There is mounting evidence which suggests that the prevalence of H. pylori infection has a strong correlation with access to clean water, suggesting a transmission route to the host. However, there are no established culture methods for the detection of viable H. pylori in the environment, in particular drinking water supplies, preventing the development of true epidemiological and risk assessments. The aim of this review is to highlight the available data to date that suggests drinking water and possible survival in biofilms as a probable transmission mode for H. pylori.

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“…We demonstrated that the icd primers were more specific for H. pylori than the species-specific protein antigen primers when tested against a variety of DNA samples. Others have used primers based on the 16S rRNA, which have also displayed good specificity when tested against many microorganisms, including several Helicobacter species (16,25), although no studies have been performed to assess the detection limit of these primers for H. pylori in feces.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a PCR Primer Based on the Isocitrate Dehydrogenase Gene for Detection of Helicobacter pylori in Feces

et al. 2000

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In order to improve detection and identification ofHelicobacter pylori in highly contaminated samples, we evaluated new specific primers based on the DNA base sequence within the isocitrate dehydrogenase (icd) gene to amplify a 1,200-bp DNA segment. The specificity of the icd primer was tested against DNA derived from various bacteria, including 7Helicobacter species and a panel of 1 gram-variable, 2 gram-positive, and 16 gram-negative bacteria, as well as DNA from houseflies and feces from H. pylori-negative patients. The primers permitted the detection of all clinical H. pyloriisolates tested, but no reactions were observed with negative controls. Several procedures for DNA extraction from feces were evaluated using PCR with icd primers. The lower limits of detection ofH. pylori DNA from two different sources containing the same number of H. pylori organisms, a pure culture and feces spiked with H. pylori, were established for each extraction method tested. The results were 8.0 × 103CFU/ml for cultures of pure H. pylori, and 8.0 × 106 CFU/ml for H. pylori from feces, using the phenol-chloroform method; 8.0 × 102 and 7.0 × 103 CFU/ml, respectively, for a glass matrix and chaotropic solution protocol; 8.0 × 102 and 7.0 × 103 CFU/ml, respectively, for the QIAamp tissue kit; and 5.0 × 102 and 5.0 × 103 CFU/ml, respectively, for the XTRAX DNA extraction kit. We conclude that the use of the icd gene as a primer for PCR represents a specific and sensitive assay for detection of H. pylori in highly contaminated samples.

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Comparison of PCR and other diagnostic techniques for detection of Helicobacter pylori infection in dyspeptic patients

Cited by 70 publications

References 34 publications

Diagnosis of Helicobacter pylori infection by PCR: comparison with other invasive techniques and detection of cagA gene in gastric biopsy specimens

Diagnosis of Helicobacter pylori infection by PCR: comparison with other invasive techniques and detection of cagA gene in gastric biopsy specimens

Transmission of Helicobacter pylori and the role of water and biofilms

Evaluation of a PCR Primer Based on the Isocitrate Dehydrogenase Gene for Detection of Helicobacter pylori in Feces

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