Comparison of Performance of Serum and Plasma in Panbio Dengue and Japanese Encephalitis Virus Enzyme-Linked Immunosorbent Assays

Blacksell, Stuart D.; Lee, Sue J.; Chanthongthip, Anisone; Taojaikong, Thaksinaporn; Thongpaseuth, Soulignasack; Hübscher, Tanja; Newton, Paul N.

doi:10.4269/ajtmh.2012.12-0043

Cited by 12 publications

(10 citation statements)

References 11 publications

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“…Our data are in line with a recent report from blood donors in Cameroon, where 5% were found to be seropositive for ZIKV IgG . The use of plasma rather than serum samples for analysis in our Rwandan donors is unlikely to have influenced the results, given the previous results from a comparative serological study of these two sample materials on similar flaviviruses . Therefore, based on its low seroprevalence, ZIKV infection could well spread and induce CZS in Rwanda, Cameroon and possibly other countries in Central Africa.…”

Section: Discussionsupporting

confidence: 91%

Seroprevalence of Zika virus and Rubella virus IgG among blood donors in Rwanda and in Sweden

Seruyange

Gahutu

Muvunyi

et al. 2018

Journal of Medical Virology

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Seroprevalence studies provide information on the susceptibility to infection of certain populations, including women of childbearing age. Such data from Central Africa are scarce regarding two viruses that cause congenital infections: Zika virus (ZIKV), an emerging mosquito-borne infection, and Rubella virus (RuV), a vaccine-preventable infection. We report on the seroprevalence of both ZIKV and RuV from Rwanda, a country without any known cases of ZIKV, but bordering Uganda where this virus was isolated in 1947. Anti-ZIKV-specific and anti-RuV-specific immunoglobulin G (IgG) antibodies were analyzed by enzyme-linked immunosorbent assay (ELISA) in serum samples from 874 Rwandan and 215 Swedish blood donors. Samples positive for IgG antibodies against ZIKV were examined for viral RNA using real-time reverse transcription polymerase chain reaction (RT-qPCR). The seroprevalence of ZIKV IgG in Rwanda was 1.4% (12/874), of which the predominance of positive findings came from the Southeastern region. All anti-ZIKV IgG-positive samples were PCR-negative. Among 297 female blood donors of childbearing age, 295 (99.3%) were seronegative and thus susceptible to ZIKV. All Swedish blood donors were IgG-negative to ZIKV. In contrast, blood donors from both countries showed high seroprevalence of IgG to RuV: 91.2% for Rwandan and 92.1% for Swedish donors. Only 10.5% (31/294) of female donors of childbearing age from Rwanda were seronegative for RuV. In Rwanda, seroprevalence for ZIKV IgG antibodies was low, but high for RuV. Hence, women of childbearing age were susceptible to ZIKV. These data may be of value for decision-making regarding prophylactic measures.

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Section: Discussionsupporting

confidence: 91%

Seroprevalence of Zika virus and Rubella virus IgG among blood donors in Rwanda and in Sweden

Seruyange

Gahutu

Muvunyi

et al. 2018

Journal of Medical Virology

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“…We examined the case serum samples at first presentation because timing of EV infection in relation to T1D disease course is very important [ 26 ]. Although serum and plasma samples provide comparable Immunoglobulin G (IgG) measures be enzyme-linked immunosorbent assay [ 27 , 28 ] and very similar for viral PCR measures [ 29 ], some studies have had lower viral detection levels in serum than plasma [ 30 , 31 ]. Thus, the excess proportion of cases with detectable EV to controls could possibly be even a little higher than reported.…”

Section: Methodsmentioning

confidence: 99%

Higher parental occupational social contact is associated with a reduced risk of incident pediatric type 1 diabetes: Mediation through molecular enteroviral indices

et al. 2018

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We aimed to examine the association between parental occupational social contact and hygiene factors on type 1 diabetes (T1D) risk and possible mediation of these effects through child enteroviral infection. We interviewed 333 incident T1D cases and 660 controls from 2008–2011 in Melbourne, Australia. Enteroviral indices (ribonucleic acid by reverse transcription polymerase chain reaction and Coxsackie B virus antibody levels) in peripheral blood were measured in nested case control samples. Parent occupational social contact was assessed by the number of well or sick children, adults or animals contacted daily through work. Higher parental occupational social contact was strongly associated with reduced T1D risk with evidence of dose response (contact with the well or sick score, Adjusted odds ratio (AOR) per category: 0.73 (95% Confidence Interval (CI): 0.66, 0.81); P<0.001 or AOR 0.63 (95% CI: 0.53, 0.75); P<0.001) respectively). Nine of the ten parental social contact indices, were significant mediated through one or more enteroviral indices. The strength of association between enterovirus presence and T1D onset increased with child age (1.2 fold increase per year; P = 0.05). Lower child hand hygiene enhanced the adverse effect of low parental occupational contact with the sick; Synergy Index 5.16 (95% CI: 3.61, 7.36). The interaction between hand washing and parental occupational contact is more consistent with protection against parental enteroviral shedding than the sharing of a protective infectious agent or microbiome.

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“…Second generation rapid assays are also now available for point-of-care diagnosis (Chaiyaratana et al, 2009;Hang et al, 2009;Zainah et al, 2009). All of these tests have now been subjected to a large number of field evaluations that have proven the value of the assay in the early diagnosis of dengue infection (Anders et al, 2012;Arya et al, 2011a, b;Bessoff et al, 2008;Blacksell et al, 2012a;Blacksell et al, 2012b;Blacksell et al, 2008;Chaiyaratana et al, 2009;Dussart et al, 2008;Fry et al, 2011;Gowri Sankar et al, 2012;Gupta et al, 2012;Hsieh and Chen, 2009;Kumarasamy et al, 2007a;Kumarasamy et al, 2007b;Lapphra et al, 2008;Lemes et al, 2005;Ludert et al, 2008;McBride, 2009;Muller et al, 2012a;Phuong et al, 2009;Shu et al, 2009;Simmons et al, 2007;Tricou et al, 2011;Xu et al, 2006;Zainah et al, 2009). The commercial development and application of NS1 detection as a diagnostic tool has revolutionised dengue diagnosis as it now provides a simple and relatively low cost assay that has high sensitivity and specificity (Castro-Jorge et al, 2010;Lima Mda et al, 2010;Wang and Sekaran, 2010b).…”

Section: Ns1 As a Diagnostic Biomarkermentioning

confidence: 99%

The flavivirus NS1 protein: Molecular and structural biology, immunology, role in pathogenesis and application as a diagnostic biomarker

2013

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The flavivirus nonstructural glycoprotein NS1 is an enigmatic protein whose structure and mechanistic function have remained somewhat elusive ever since it was first reported in 1970 as a viral antigen circulating in the sera of dengue-infected patients. All flavivirus NS1 genes share a high degree of homology, encoding a 352-amino-acid polypeptide that has a molecular weight of 46-55 kDa, depending on its glycosylation status. NS1 exists in multiple oligomeric forms and is found in different cellular locations: a cell membrane-bound form in association with virus-induced intracellular vesicular compartments, on the cell surface and as a soluble secreted hexameric lipoparticle. Intracellular NS1 co-localizes with dsRNA and other components of the viral replication complex and plays an essential cofactor role in replication. Although this makes NS1 an ideal target for inhibitor design, the precise nature of its cofactor function has yet to be elucidated. A plethora of potential interacting partners have been identified, particularly for the secreted form of NS1, with many being implicated in immune evasion strategies. Secreted and cell-surface-associated NS1 are highly immunogenic and both the proteins themselves and the antibodies they elicit have been implicated in the seemingly contradictory roles of protection and pathogenesis in the infected host. Finally, NS1 is also an important biomarker for early diagnosis of disease. In this article, we provide an overview of these somewhat disparate areas of research, drawing together the wealth of data generated over more than 40 years of study of this fascinating protein.

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Comparison of Performance of Serum and Plasma in Panbio Dengue and Japanese Encephalitis Virus Enzyme-Linked Immunosorbent Assays

Cited by 12 publications

References 11 publications

Seroprevalence of Zika virus and Rubella virus IgG among blood donors in Rwanda and in Sweden

Seroprevalence of Zika virus and Rubella virus IgG among blood donors in Rwanda and in Sweden

Higher parental occupational social contact is associated with a reduced risk of incident pediatric type 1 diabetes: Mediation through molecular enteroviral indices

The flavivirus NS1 protein: Molecular and structural biology, immunology, role in pathogenesis and application as a diagnostic biomarker

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