Comparison of phenolic compound accumulation profiles in eight evergreen woody core eudicots indicating the diverse ecological adaptability of Camellia sinensis

Jiang, Xiaolan; Hou, Hua; Zhang, Shuxiang; Liu, Yajun; Wang, Haiyan; Deng, Wei‐Wei; Zhou, Shasha; Wu, Yahui; Shen, Fangfang; Gao, Liping; Xia, Tao

doi:10.1016/j.scienta.2017.03.018

Cited by 14 publications

(8 citation statements)

References 42 publications

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“…These results highlight the functional importance of the phenolic compounds in the plant response to environmental changes due to their antioxidant activity [13,76]. For example, a high concentration of these compounds could represent an ecological response to different forms of biotic and abiotic stress [77].…”

Section: Biochemical Compounds Affecting the Growth Of Resproutsmentioning

confidence: 78%

How Do Plants Respond Biochemically to Fire? The Role of Photosynthetic Pigments and Secondary Metabolites in the Post-Fire Resprouting Response

Santacruz‐García

Bravo

Corro

et al. 2021

Forests

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Resprouting is one of the main regeneration strategies in woody plants that allows post-fire vegetation recovery. However, the stress produced by fires promotes the biosynthesis of compounds which could affect the post-fire resprouting, and this approach has been poorly evaluated in fire ecology. In this study, we evaluate the changes in the concentration of chlorophylls, carotenoids, phenolic compounds, and tannins as a result of experimental burns (EB). We asked whether this biochemical response to fire could influence the resprouting responses. For that, we conducted three EB in three successive years in three different experimental units. Specifically, we selected six woody species from the Chaco region, and we analyzed their biochemical responses to EB. We used spectrophotometric methods to quantify the metabolites, and morphological variables to estimate the resprouting responses. Applying a multivariate analysis, we built an index to estimate the biochemical response to fire to EB per each species. Our results demonstrate that photosynthetic pigment concentration did not vary significantly in burnt plants that resprout in response to EB, whereas concentrations of secondary metabolites (phenolic compounds and tannins) increased up to two years after EB. Our main results showed that phenolic compounds could play a significant role in the resprouting responses, while photosynthetic pigments seem to have a minor but significant role. Such results were reaffirmed by the significant correlation between the biochemical response to fire and both resprouting capacity and resprouting growth. However, we observed that the biochemical response effect on resprouting was lower in tree species than in shrubby species. Our study contributes to the understanding of the biochemical responses that are involved in the post-fire vegetation recovery.

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Section: Biochemical Compounds Affecting the Growth Of Resproutsmentioning

confidence: 78%

How Do Plants Respond Biochemically to Fire? The Role of Photosynthetic Pigments and Secondary Metabolites in the Post-Fire Resprouting Response

Santacruz‐García

Bravo

Corro

et al. 2021

Forests

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“…The rPA concentration in the supernatant and precipitate (dissolved by 0.5 mL 10 mM Na 2 EDTA solution) was stained with 50% (v/v) H 2 SO 4 −water solution containing 1% vanillin (w/v) according to the procedure reported by Jiang et al 43 HPLC was also used to identify EC and rPA in the supernatant and precipitate as the same study of Jiang et al 43 The gradient of the 1% acetic acid mobile phase was changed from 95% to 90% (v/v) for 5 min, to 75% for 15 min, to 60% for 20 min, and to 95% for 22 min and was maintained for 3 to 25 min.…”

Section: Methodsmentioning

confidence: 99%

Proanthocyanidin–Aluminum Complexes Improve Aluminum Resistance and Detoxification of Camellia sinensis

Jiang

et al. 2020

J. Agric. Food Chem.

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Aluminum (Al) influences crop yield in acidic soil. The tea plant (Camellia sinensis) has high Al tolerance with abundant monomeric catechins in its leaves, especially epigallocatechin gallate (EGCG), and polymeric proanthocyanidins in its roots (rPA). The role of these polyphenols in the Al resistance of tea plants is unclear. In this study, we observed that these polyphenols could form complexes with Al in vitro, and complexation capacity was positively influenced by high solution pH (pH 5.8), polyphenol type (rPA and EGCG), and high Al concentration. In the 27Al nuclear magnetic resonance (NMR) experiment, rPA-Al and EGCG-Al complex signals could be detected both in vitro and in vivo. The rPA-Al and EGCG-Al complexes were detected in roots and old leaves, respectively, of both greenhouse seedlings and tea garden plants. Furthermore, in seedlings, Al accumulated in roots and old leaves and mostly existed in the apoplast in binding form. These results indicate that the formation of complexes with tea polyphenols in vivo plays a vital role in Al resistance in the tea plant.

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“…Acetone was added to the supernatant to a total volume of 6 mL. The vanillin−H 2 SO 4 method 28 was used to measure total flavonoids with some modifications: 0.05 mL of flavonoid supernatant was added to 2 mL of 1% (w/v) vanillin−50% sulfuric acid solution, and after 15 min of reaction at room temperature, the relative content of total flavonoids was obtained by measuring the absorbance at 500 nm. The standard curve was drawn based on the EC standard.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Nitrogen-Regulated Theanine and Flavonoid Biosynthesis in Tea Plant Roots: Protein-Level Regulation Revealed by Multiomics Analyses

Yan

Cheng

Yang

et al. 2021

J. Agric. Food Chem.

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Theanine and flavonoids (especially proanthocyanidins) are the most important and abundant secondary metabolites synthesized in the roots of tea plants. Nitrogen promotes theanine and represses flavonoid biosynthesis in tea plant roots, but the underlying mechanism is still elusive. Here, we analyzed theanine and flavonoid metabolism in tea plant roots under nitrogen deficiency and explored the regulatory mechanism using proteome and ubiquitylome profiling together with transcriptome data. Differentially expressed proteins responsive to nitrogen deficiency were identified and found to be enriched in flavonoid, nitrogen, and amino acid metabolism pathways. The proteins responding to nitrogen deficiency at the transcriptional level, translational level, and both transcriptional and translational levels were classified. Nitrogen-deficiency-responsive and ubiquitinated proteins were further identified. Our results showed that most genes encoding enzymes in the theanine synthesis pathway, such as CsAlaDC, CsGDH, and CsGOGATs, were repressed by nitrogen deficiency at transcriptional and/or protein level(s). While a large number of enzymes in flavonoid metabolism were upregulated at the transcriptional and/or translational level(s). Importantly, the ubiquitylomic analysis identified important proteins, especially the hub enzymes in theanine and flavonoid biosynthesis, such as CsAlaDC, CsTSI, CsGS, CsPAL, and CsCHS, modified by ubiquitination. This study provided novel insights into the regulation of theanine and flavonoid biosynthesis and will contribute to future studies on the post-translational regulation of secondary metabolism in tea plants.

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Comparison of phenolic compound accumulation profiles in eight evergreen woody core eudicots indicating the diverse ecological adaptability of Camellia sinensis

Cited by 14 publications

References 42 publications

How Do Plants Respond Biochemically to Fire? The Role of Photosynthetic Pigments and Secondary Metabolites in the Post-Fire Resprouting Response

How Do Plants Respond Biochemically to Fire? The Role of Photosynthetic Pigments and Secondary Metabolites in the Post-Fire Resprouting Response

Proanthocyanidin–Aluminum Complexes Improve Aluminum Resistance and Detoxification of Camellia sinensis

Nitrogen-Regulated Theanine and Flavonoid Biosynthesis in Tea Plant Roots: Protein-Level Regulation Revealed by Multiomics Analyses

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