Comparison of Phenotypic with Genotypic Procedures for Confirmation of Coagulase-Negative
            <i>Staphylococcus</i>
            Catheter-Related Bloodstream Infections

Aldea-Mansilla, Carmen; Viedma, Darío García de; Cercenado, Emilia; Martín‐Rabadán, Pablo; Marı́n, Mercedes; Bouza, Emilio

doi:10.1128/jcm.00839-06

Cited by 21 publications

(13 citation statements)

References 30 publications

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“…In fact, previous reviews of CNS endocarditis have discussed the spectrum of disease from the perspective of phenotypic identifications with limited attention to specific species (17,22). We found S. epidermidis as the most common CNS species, an observation corroborated by previous reports on non-endocarditis patients with invasive CNS disease (1,3,10,16,19). The rank order of non-S. epidermidis infections differs in our report from that in others.…”

Section: Discussionsupporting

confidence: 90%

“…The rank order of non-S. epidermidis infections differs in our report from that in others. We report S. lugdunensis as the second most common pathogen, whereas non-endocarditis studies have observed S. hominis, S. haemolyticus, and S. capitis as the next most commonly encountered CNS pathogens (1,19,21). The clinical implications of more accurate identification of CNS by gene sequencing are not fully known.…”

Section: Discussionmentioning

confidence: 84%

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Genotypic Diversity of Coagulase-Negative Staphylococci Causing Endocarditis: a Global Perspective

et al. 2008

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Coagulase-negative staphylococci (CNS) are important causes of infective endocarditis (IE), but their microbiological profiles are poorly described. We performed DNA target sequencing and susceptibility testing for 91 patients with definite CNS IE who were identified from the International Collaboration on Endocarditis-Microbiology, a large, multicenter, multinational consortium. A hierarchy of gene sequences demonstrated great genetic diversity within CNS from patients with definite endocarditis that represented diverse geographic regions. In particular, rpoB sequence data demonstrated unique genetic signatures with the potential to serve as an important tool for global surveillance.Coagulase-negative staphylococci (CNS) are increasingly important causes of community-and health care-associated infective endocarditis (7,12,17). Although more than 40 species make up this heterogeneous group of microorganisms, identification of CNS to the species level often is not performed because of laboratory uncertainty about its clinical relevance or the absence of reliable identification systems (10). There is, however, growing evidence that identification of CNS to the species level may alter diagnostic and therapeutic clinical decision making where specific species have unique virulence factors (e.g., Staphylococcus lugdunensis) (2) or unusual antibacterial resistance patterns (e.g., glycopeptide resistance with S. epidermidis and S. haemolyticus) (4-6). In recent years, partial 16S rRNA gene sequencing has emerged as an accurate and reliable method to identify CNS, but this molecular target is limited by having less than 1% sequence divergence among some CNS species. Alternative gene targets such as tuf (elongation factor Tu) (10) and rpoB (RNA polymerase ␤ subunit) (9, 14) have been evaluated, but to our knowledge, no studies have applied gene sequencing of these targets from patients with definite CNS endocarditis.Given the limited frequency of CNS endocarditis in a single institution, the International Collaboration on EndocarditisMicrobiology (ICE-Micro), a large, multicenter, multinational consortium, provided a unique opportunity to improve our understanding of the spectrum of CNS microorganisms implicated in prosthetic and native valve endocarditis. We performed gene sequencing with multiple DNA targets to identify CNS from patients with definite endocarditis to the species level. We also evaluated the potential for a hierarchy of sequence data to provide greater specificity for species identification, serve as an epidemiologic tool to assess clonality, and predict antimicrobial resistance. MATERIALS AND METHODSCNS isolates from patients with definite endocarditis were submitted by ICEMicro investigators representing a collection from 18 medical centers in 12 countries. Conventional identification and susceptibility testing were performed at a central laboratory with a commercially available panel processed on the Microscan Walkaway instrument (PC-21; Dade Behring, Deerfield, IL) by a

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 84%

Genotypic Diversity of Coagulase-Negative Staphylococci Causing Endocarditis: a Global Perspective

et al. 2008

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“…S. epidermidis is the most clinically relevant species among the coagulase-negative staphylococci, accounting for Ͼ70% of catheter-related infections within the group (7,8). Therefore, we focused on S. epidermidis for our studies but maintained an emphasis on targets conserved across the entire genus.…”

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confidence: 99%

Antibodies to PhnD Inhibit Staphylococcal Biofilms

et al. 2014

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Biofilm formation on central lines or peripheral catheters is a serious threat to patient well-being. Contaminated vascular devices can act as a nidus for bloodstream infection and systemic pathogen dissemination. Staphylococcal biofilms are the most common cause of central-line-associated bloodstream infections, and antibiotic resistance makes them difficult to treat. As an alternative to antibiotic intervention, we sought to identify anti-staphylococcal biofilm targets for the development of a vaccine or antibody prophylactic. A screening strategy was devised using a microfluidic system to test antibody-mediated biofilm inhibition under biologically relevant conditions of shear flow. Affinity-purified polyclonal antibodies to target antigen PhnD inhibited both Staphylococcus epidermidis and S. aureus biofilms. PhnD-specific antibodies blocked biofilm development at the initial attachment and aggregation stages, and deletion of phnD inhibited normal biofilm formation. We further adapted our microfluidic biofilm system to monitor the interaction of human neutrophils with staphylococcal biofilms and demonstrated that PhnD-specific antibodies also serve as opsonins to enhance neutrophil binding, motility, and biofilm engulfment. These data support the identification of PhnD as a lead target for biofilm intervention strategies performed either by vaccination or through passive administration of antibodies.

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“…There is therefore a risk of propagation of drug resistance strains to other hospitals and the community. The number of infections due to staphylococci, notably coagulase negative ones is in constant increase these last decades [19].…”

Section: Discussionmentioning

confidence: 99%

Comparative Study of Infectious Risks in Diagnostic Laboratories between Public and Private Hospitals in Benin

Bankolè¹,

Dougnon²

2015

J Med Microb Diagn

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Introduction: Laboratory associated infections are serious occupational hazards for laboratory workers who are exposed through various routes. The present study aimed to compare the bacteriological risks encountered at diagnostic laboratories in public and private hospitals in Southern Benin. Methodology:A scorecard of laboratory practices was developed based on WHO laboratory inspection checklist. The private laboratory was split into two sections B1 and B2 and the public in C1 to C5. A total of 125 swabs from hand, cell phones, work surfaces and door knobs were collected from all laboratories and submitted to bacteriological analyses. Results:Apart from some sections of the public laboratories where poor sanitation was noticed, the overall hygiene level are satisfactory in both hospitals even though the private laboratories were significantly safer (p<0.05) than the public ones (68.55% and 55.81% respectively). Bacteriological investigations showed that mobile phones were the most contaminated items in the private labs while work surfaces harboured more germs in the public laboratories.Although private laboratories were contaminated by over 11 bacteria species against 5 from the public labs, coagulase negative Staphylococcus were the most prevalent isolated organisms from both hospitals. All the isolated bacteria form public and private laboratories were multidrug resistant. Conclusions:Though the hygiene level in the private labs was better than in public labs, the risk of lobaratory associated infections is rampant in both areas with respect to isolated organisms. Serious safety instructions and monitoring must be set to avoid worse situations.

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Comparison of Phenotypic with Genotypic Procedures for Confirmation of Coagulase-Negative Staphylococcus Catheter-Related Bloodstream Infections

Cited by 21 publications

References 30 publications

Genotypic Diversity of Coagulase-Negative Staphylococci Causing Endocarditis: a Global Perspective

Genotypic Diversity of Coagulase-Negative Staphylococci Causing Endocarditis: a Global Perspective

Antibodies to PhnD Inhibit Staphylococcal Biofilms

Comparative Study of Infectious Risks in Diagnostic Laboratories between Public and Private Hospitals in Benin

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