Comparison of Physical Maps of Ribosomal DNA Repeating Units in Pythium, Phytophthora and Apodachlya

Klassen, Glen R.; McNabb, Sally A.; Dick, M.W.

doi:10.1099/00221287-133-10-2953

Cited by 12 publications

(5 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The DNA of Pythium, isolated on a CsC1-bisbenzimide density gradient, showed three bands under UV light, as found by Klassen et al (1987). The top and bottom bands fluoresced blue while the middle band fluoresced green.…”

Section: Dna Banding Patternsmentioning

confidence: 92%

See 1 more Smart Citation

Comparative Studies on the DNA of Pythium Species and Some Possibly Related Taxa

Belkhiri

Dick

1988

Microbiology

Self Cite

View full text Add to dashboard Cite

DNA from 14 Pythium species, Verrucalvus jlavofaciens and Zoophagus insidians was characterized by CsC1-bisbenzimide density gradients in order to investigate its taxonomic potential. A few incomplete analyses were made for other species. All clearly assignable Pythium species produced three DNA bands in the gradient. Pythium undulatum along with Verrucalvus and Zoophagus produced only two bands. Another possible exception, which needs further investigation, is P. vexans. The DNA had a relatively constant banding pattern in CsCl gradients. The small number (eight) of DNA criteria that were available were subjected to cluster analysis to assess the relationships between replicates and species. This restricted database, similar in size to the number of criteria used in morphological taxonomy, provided an independent assessment of the values that have been attached to generic and subgeneric classifications. This approach enabled assessments to be made of relationships between species that have incomplete life-histories and which therefore lack features essential for traditional taxonomic decisions.

show abstract

Section: Dna Banding Patternsmentioning

confidence: 92%

“…diclinum and P . torulosum, are closely related on other DNA criteria (McNabb et al, 1987; Klassen et al, 1987). Use of species means (Fig.…”

Section: Dna Base Composition and Chr-dna Hyperchromicity (% A260)mentioning

confidence: 99%

Comparative Studies on the DNA of Pythium Species and Some Possibly Related Taxa

Belkhiri

Dick

1988

Microbiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Schroeter, and other organisms. Restriction fragment length polymorphisms (RFLPs) of nuclear DNA (e.g., internal transcribed spacer, ITS region) and mitochondrial DNA were used to identify individual species of Phytophthora (Förster et al 1990;Förster and Coffey 1993;Tooley et al 1996), Pythium Pringsheim (Klassen et al 1987;Belkhiri and Dick 1988;Martin and Kistler 1990;Chen and Hoy 1993;Rafin et al 1995) and Saprolegnia Nees (Molina et al 1995). Kageyama (1997) sequenced the ITS region and was able to design species-specific primers for detecting Pythium ultimum Trow from diseased seedlings.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic studies of Saprolegniomycetidae and related groups based on nuclear large subunit ribosomal DNA sequences

Riethmüller¹,

Weiß²,

Oberwinkler³

2000

Can. J. Bot.

View full text Add to dashboard Cite

To reveal phylogenetic relationships within the Peronosporomycetes (Oomycetes), we sequenced a part of the nuclear rDNA coding for the ribosomal large subunit of 46 Peronosporomycetes species and one representative of the Xanthophyta. The main emphasis of our study was put on the phylogenetic relationships within the Saprolegniomycetidae. We supplemented our data with a sequence of Phytophthora megasperma Drechsler from GenBank. Two sets of sequences were analysed using the neighbor-joining method, statistically supported by the bootstrap method, as well as the maximum parsimony method. Our results are well compatible with the tripartite subclassification of the Peronosporomycetes into Saprolegniomycetidae, Rhipidiomycetidae and Peronosporomycetidae, as well as with the placement of the orders Saprolegniales and Leptomitales in the Saprolegniomycetidae. Pachymetra chaunorhiza Croft & Dick, which has been placed in the Sclerosporales, was grouped within the Saprolegniales. Within the Peronosporomycetidae, the orders Peronosporales and Pythiales could not be separated. There are indications that Phytophthora de Bary and the Peronosporales form a common natural group. The genus Achlya Nees proved to be a heterogeneous group.

show abstract

“…Pure cultures of Phytophthora species were grown on 5% CV8 agar for 3 to 5 days and a single agar plug (5 mm diameter) was transferred to a petri plate containing 10% CV8 broth. Cultures were allowed to grow at room temperature for 3 to 5 days into thick mycelial mats and then collected by vacuum filtration and rinsed with distilled water through Fisherbrand plain filter paper (Fisher Scientific, Waltham, MA, USA) (Klassen et al 1987). The mycelium was stored in 2 ml microcentrifuge tubes at -20 C (-4 F) until processed.…”

Section: Methodsmentioning

confidence: 99%

Annuals and Herbaceous Perennials Tolerant or Resistant to Phytophthora Species in the Landscape1

Henson¹,

Sharpe²,

Meadows³

2020

Journal of Environmental Horticulture

View full text Add to dashboard Cite

Plants of one or two cultivars of 16 annuals and 14 herbaceous perennials were evaluated based on desirability and anecdotal evidence of resistance to Phytophthora root or crown rot. Six plant cultivars served as susceptible controls. Three landscape beds were established in North Carolina and each was infested with three species of Phytophthora: P. nicotianae, P. drechsleri, and P. tropicalis. Plants were regularly rated for disease incidence and symptomatic plants were assayed to determine the presence of Phytophthora species. Ten cultivars of annuals and seven cultivars of herbaceous perennials did not exhibit symptoms of Phytophthora root or crown rot or other disease throughout the season (June 4 to October 15, 2018). Phytophthora spp. were recovered from seven and six cultivars of the evaluated annuals and herbaceous perennials, respectively. Phytophthora nicotianae, P. drechsleri, or P. cryptogea were recovered from a susceptible host in each landscape bed. P. tropicalis was recovered from one plant cultivar evaluated. Phytophthora cryptogea was recovered from three plant cultivars, although this species was not intentionally introduced in the landscape beds. We identified 22 plant cultivars within 13 herbaceous plant species that grew vigorously in landscape beds infested with species of Phytophthora. Index words: bedding plants, disease resistance, herbaceous perennials, landscape plants, Phytophthora nicotianae, Phytophthora drechsleri, Phytophthora tropicalis. Species used in this study: yarrow (Achillea millefolium L. ‘Desert Eve Red'), fernleaf yarrow (Achillea filipendulina Lam. ‘Moonshine Yellow'), angelonia (Angelonia angustifolia Benth. ‘ArchAngel Pink', ‘Serenita White'), annual vinca (Catharanthus roseus (L.) G. Don ‘Cora Apricot', ‘Cora Strawberry', ‘Pacifica Raspberry'), celosia (Celosia argentea L. ‘New Look'), tickseed (Coreopsis auriculata L. ‘Nana', ‘Yellow Jethro Tull'), purple coneflower (Echinacea purpurea (L.) Moench ‘Cheyenne Spirit', ‘PowWow Wild Berry'), blanket flower (Gaillardia x grandiflora Hort. ‘Goblin', ‘Mesa Bi-color'), Barberton daisy (Gerbera jamesonii Bolus ex Hooker f. ‘Crazy Daisy'), verbena (Glandularia canadensis ‘Homestead Purple'), >dusty miller (Jacobaea maritima (L.) Pelser & Meijden ‘Silver Dust'), New Guinea impatiens (Impatienshawkeri W.Bull ‘Hamony', ‘Sunpatiens Compact Orchid', ‘Sunpatiens Lilac'), sweet potato vine (Ipomoea batatas (L.) Lam. ‘Ace of Spades', ‘Bright Idea Tri-color'), West Indian lantana (Lantana camara L. ‘Miss Huff'), lantana (Lantana x hybrida ‘New Gold'), shasta daisy (Leucanthemum x superbum (Bergmans ex J.W. Ingram) Bergmans ex Kent. ‘Becky', ‘Snow Lady'), bee balm (Monarda didyma L. ‘Petite Delight', ‘Jacob Cline'), ornamental grass (Panicum virgatum L. ‘Rotstrahlbusch', ‘Shenandoah'), geranium (Pelargonium x hortorum L.H. Bailey (pro. sp.) ‘Bullseye Cherry', Calliope Dark Red'), calibrachoa (Petunia x calibrachoa ‘Super Cal'), petunia (Petunia x hybrida (Hooker) Vilmorin ‘Easy Wave Red', ‘Easy Wave White', ‘Wave Purple', ‘Yellow Madness', Violet Picotee'), annual phlox (Phlox drummondii Hook. ‘Intensia Red Hot', ‘Phlox Star'), garden phlox (Phlox paniculata L. ‘Amethyst True Gal'), black-eyed susan (Rudbeckia hirta L. ‘Indian Summer', ‘Prairie Sun'), mealy blue sage (Salvia farinacea Benth. ‘Victoria Blue'), African marigold (Tagetes erecta L. ‘Inca Yellow', ‘Proud Yellow'), French marigold (Tagetes patula L. ‘Disco Mix', ‘Disco Yellow'), narrowleaf zinnia (Zinnia angustifolia Kunth. ‘Star Orange', ‘Star White'), Phytophthora nicotianae Breda de Haan, Phytophthora cryptogea Pethybr. and Laff, Phytophthora drechsleri Tucker, Phytophthora tropicalis Aragaki and J.Y. Uchida, zinnia (Zinnia elegans Jacq. ‘Magellan Orange').

show abstract

Comparison of Physical Maps of Ribosomal DNA Repeating Units in Pythium, Phytophthora and Apodachlya

Cited by 12 publications

References 15 publications

Comparative Studies on the DNA of Pythium Species and Some Possibly Related Taxa

Comparative Studies on the DNA of Pythium Species and Some Possibly Related Taxa

Phylogenetic studies of Saprolegniomycetidae and related groups based on nuclear large subunit ribosomal DNA sequences

Annuals and Herbaceous Perennials Tolerant or Resistant to Phytophthora Species in the Landscape1

Contact Info

Product

Resources

About