Comparison of Phytochemical Composition and Biological Activities of <i>Rubus ulmifolius</i> Extracts Originating from Four Regions of Tunisia

Tabarki, Sonia; Aouadhi, Chedia; Mechergui, Kaouther; Hammi, Khaoula Mkadmini; Ksouri, Riadh; Raïes, Aly; Toumi, Lamjed

doi:10.1002/cbdv.201600168

Cited by 10 publications

(4 citation statements)

References 40 publications

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“…The top-performing preparation in our study was a black raspberry extract from the University of Minnesota (UMB-BRB-E), which achieved complete H. pylori growth inhibition at (>90%) at 0.5% (5 mg/mL). This inhibitory concentration is similar to or lower than those of Rubus extracts described in other studies [16,32,42], but several log folds higher than standard antibiotics such as amoxicillin and clarithromycin [43] or the metronidazole used in our study, which completely blocked H. pylori growth at 136 µg/mL.…”

Section: Discussionsupporting

confidence: 87%

A High-Throughput Metabolic Microarray Assay Reveals Antibacterial Effects of Black and Red Raspberries and Blackberries against Helicobacter pylori Infection

et al. 2021

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Helicobacter pylori infection is commonly treated with a combination of antibiotics and proton pump inhibitors. However, since H. pylori is becoming increasingly resistant to standard antibiotic regimens, novel treatment strategies are needed. Previous studies have demonstrated that black and red berries may have antibacterial properties. Therefore, we analyzed the antibacterial effects of black and red raspberries and blackberries on H. pylori. Freeze-dried powders and organic extracts from black and red raspberries and blackberries were prepared, and high-performance liquid chromatography was used to measure the concentrations of anthocyanins, which are considered the major active ingredients. To monitor antibiotic effects of the berry preparations on H. pylori, a high-throughput metabolic growth assay based on the Biolog system was developed and validated with the antibiotic metronidazole. Biocompatibility was analyzed using human gastric organoids. All berry preparations tested had significant bactericidal effects in vitro, with MIC90 values ranging from 0.49 to 4.17%. Antimicrobial activity was higher for extracts than powders and appeared to be independent of the anthocyanin concentration. Importantly, human gastric epithelial cell viability was not negatively impacted by black raspberry extract applied at the concentration required for complete bacterial growth inhibition. Our data suggest that black and red raspberry and blackberry extracts may have potential applications in the treatment and prevention of H. pylori infection but differ widely in their MICs. Moreover, we demonstrate that the Biolog metabolic assay is suitable for high-throughput antimicrobial susceptibility screening of H. pylori.

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Section: Discussionsupporting

confidence: 87%

A High-Throughput Metabolic Microarray Assay Reveals Antibacterial Effects of Black and Red Raspberries and Blackberries against Helicobacter pylori Infection

et al. 2021

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“…The top-performing preparation in our study was a black raspberry extract from the University of Minnesota (UMB-BRB-E), which achieved complete H. pylori growth inhibition at (>90%) at 0.5 % (5 mg/mL). This inhibitory concentration is similar to or lower than those of Rubus extracts described in other studies [16, 38, 46], but several log folds higher than standard antibiotics such as amoxicillin and clarithromycin [47].…”

Section: Discussionsupporting

confidence: 85%

A high-throughput metabolic microarray assay reveals antibacterial effects of black and red raspberries and blackberries againstHelicobacter pyloriinfection

Goodman

Lyon

Scotto

et al. 2020

Preprint

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Helicobacter pylori is an important bacterial pathogen that causes chronic infection of the human stomach, leading to gastritis, peptic ulcer disease and gastric cancer. Treatment with appropriate antibiotics can eliminate H. pylori infection and reduce the risk for severe disease outcomes. However, since H. pylori is becoming increasingly resistant to standard antibiotic regimens, novel treatment strategies are needed. Previous studies have demonstrated that black and red berries may have antibacterial properties. Therefore, we analyzed organic extracts and powders from black and red raspberries and blackberries and determined their antibacterial effects on multiple H. pylori strains. We used high-performance liquid chromatography to measure berry anthocyanins, which are considered the major active ingredients. To monitor antibiotic effects of the berry preparations on H. pylori, we developed a high-throughput metabolic growth assay based on the OmniLog™ system. All berry preparations tested had significant bactericidal effects in vitro, with MIC90 values ranging from 0.49 to 4.17%. We next used human gastric epithelial organoids to evaluate biocompatibility of the berry preparations and showed that black raspberry extract, which had the strongest antimicrobial activity, was non-toxic at the concentration required for complete bacterial growth inhibition. To determine whether dietary black raspberry application could eliminate H. pylori infection in vivo, mice were infected with H. pylori and then were placed on a diet containing 10% black raspberry powder. However, this treatment did not significantly impact bacterial infection rates or gastric pathology. In summary, our data indicate that black and red raspberry and blackberry products have potential applications in the treatment and prevention of H. pylori infection, because of their antibacterial effects and good biocompatibility. However, delivery and formulation of berry compounds needs to be optimized to achieve significant antibacterial effects in vivo.

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“…Previous investigations [ 51 , 52 ] have indicated that the phenols and flavonoids present in the plants may have an influence on the microorganism growth. Generalić et al [ 51 ] reported that leaf extracts of Salvia officinalis L. contained high amounts of phenols and flavonoids exhibiting high antibacterial activity, especially against E. coli , S. aureus , and B. cereus .…”

Section: Discussionmentioning

confidence: 99%

Bioactivities of Traditional Medicinal Plants in Alexandria

Elansary

Szopa

Kubica

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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In traditional folklore, medicinal herbs play a vital role in the prevention and treatment of microbial diseases. In the present study, the phenolic profiles of the medicinal plants Asparagus aethiopicus L., Citrullus colocynthis L., Senna alexandrina L., Kalanchoe delagoensis L., Gasteria pillansii L., Cymbopogon citratus, Brassica juncea, and Curcuma longa L. were determined by high-performance liquid chromatography with a diode-array detector method. The results revealed rich sources of important compounds such as robinin in the fruits and leaves of A. aethiopicus; caffeic acid in the tubers of A. aethiopicus and quercitrin in the leaves of G. pillansii. Further, relatively high antioxidant, antibacterial, and antifungal activities were observed in C. colocynthis fruit coat, S. alexandrina pods, and A. aethiopicus leaves, respectively. The relatively higher the bioactivities of plants extracts associated with the phenols in these plants, in particular, the more abundant the phenols. Therefore, it was concluded that the fruit coat of C. colocynthis, pods of S. alexandrina, and leaves of A. aethiopicus might be excellent sources of natural products. These plant extracts also have a wide spectrum of antimicrobial activities that could be used in the pharmaceutical industries and to control diseases.

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Comparison of Phytochemical Composition and Biological Activities of Rubus ulmifolius Extracts Originating from Four Regions of Tunisia

Cited by 10 publications

References 40 publications

A High-Throughput Metabolic Microarray Assay Reveals Antibacterial Effects of Black and Red Raspberries and Blackberries against Helicobacter pylori Infection

A High-Throughput Metabolic Microarray Assay Reveals Antibacterial Effects of Black and Red Raspberries and Blackberries against Helicobacter pylori Infection

A high-throughput metabolic microarray assay reveals antibacterial effects of black and red raspberries and blackberries againstHelicobacter pyloriinfection

Bioactivities of Traditional Medicinal Plants in Alexandria

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