Comparison of Phytochemical Profile and Bioproperties of Methanolic Extracts from Different Parts of Tunisian Rumex roseus

Abstract

The genus Rumex (Polygonaceae) is distributed worldwide and the different species belonging to it are used in traditional medicine. The present study aimed at the evaluation of the phytochemical profile and the biochemical properties of methanolic extracts from different parts (roots, stems, and leaves) of Rumex roseus, a wild local Tunisian plant traditionally used as food. The phytochemical analysis on the extracts was performed using standard colorimetric procedures, HPLC‐DAD, and HPLC‐DAD‐ESI‐MS; then, several in vitro cell‐free assays have been used to estimate their antioxidant/free radical scavenging capability (TAC‐PM, DPPH, TEAC, FRAP, ORAC, SOD‐like activity, and HOCl‐induced albumin degradation). Additionally, anti‐inflammatory effect of these extracts was evaluated in an in vitro model of acute intestinal inflammation in differentiated Caco‐2 cells. The results showed that the methanolic extracts from stems and, especially, leaves contain substantial amounts of flavones (apigenin and luteolin, together with their derivatives), while the extract from roots is characterized by the presence of tannins and quinic acid derivatives. All the extracts appeared endowed with excellent antioxidant/free radical scavenging properties. In particular, the extract from roots was characterized by a remarkable activity, probably due to its different and peculiar polyphenolic composition. Furthermore, both Rumex roseus roots and stems extracts demonstrated an anti‐inflammatory effect in intestinal epithelial cells, reducing TNF‐α‐induced gene expression of IL‐6 and IL‐8. In conclusion, R. roseus methanolic extracts have shown to be potential sources of bioactive compounds to be used in the prevention and treatment of pathologies related to oxidative stress and inflammation.

The genus Rumex (Polygonaceae): an ethnobotanical, phytochemical and pharmacological review

“…The higher dose of extract (150 mg/kg) showed inhibition (41.7%) of edema in rats compared with the positive control, indomethacin (10 mg/kg, 36.6%) [21]. Methanolic extracts of the roots and stems of R. roseus exhibited anti-inflammatory functions in intestinal epithelial cells, reducing TNF-α-induced gene expression of IL-6 and IL-8 [154].…”

“…The roots of R. thyrsiflorus [164], ethanol extracts of R. obtusifolius and R. crispus showed antioxidant ability on DPPH, ABTS + and FRAP assays [165]. Moreover, R. tingitanus leaves, R. dentatus, R. rothschildianus leaves, R. roseus and R. vesicarius also showed antioxidant activity on DPPH assay [13,78,105,154,166,167]. Phenolics isolated from R. tunetanus flowers and stems displayed antioxidant properties on DPPH and FRAP assays [103].…”

Phenolic Profiles, Antioxidant and Anti-Inflammatory Activities of Hydrodistillation Wastewaters from Five Lamiaceae Species

“…The free radical scavenging ability of the samples (lyophilized wastewaters) was tested by the DPPH radical scavenging assay according to Chelly et al [ 51 ]. Samples were solubilized in MeOH and 37.5 μL of the prepared concentrations (range: 0.125–1 mg/mL) were separately mixed with 1.5 mL of a DPPH methanolic solution (100 μM).…”

Trachystemon orientalis L. Ekstraktlarının Kozmetik Endüstrisinde Kullanım Potansiyeli: Cilt Koruyucu, Güneş Koruyucu ve Yaşlanma Karşıtı İçerikleri

“…Tyrosinase inhibitory activity of many plant extracts was carried out to find new sources of anti-tyrosinase compounds [41]. Due to the increasing use of herbal resources in cosmetic products, tyrosinase inhibitory activities of different parts of the herbal material have been reported by several studies [42][43][44]. In particular, medicinal plants from traditional medicine systems have the potential to be used for the treatment of hyperpigmentation and as tyrosinase inhibitors [45].…”