Comparison of PI-PLC based assays and PCR along with in vivo pathogenicity tests for rapid detection of pathogenic Listeria monocytogenes

Aurora, Ritu; Prakash, Alka; Prakash, Soam; Rawool, Deepak B.; Barbuddhe, Sukhadeo B.

doi:10.1016/j.foodcont.2007.07.002

Cited by 25 publications

(18 citation statements)

References 33 publications

(34 reference statements)

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“…However, it is advisable to perform an alternative in vitro test such as detecting PI-PLC activity on ALOA medium. This method besides detecting PI-PLC activity also allows discrimination between the pathogenic species of Listeria from the non-pathogenic listeriae (Aurora et al, 2008). In the present investigation almost similar observation were seen for PI-PLC activity on ALOA medium as that observed for hemolytic titre for the recovered L. monocytogenes isolates.…”

Section: Discussionsupporting

confidence: 88%

“…Also detection of hly and plcA gene by PCR in L. monocytogenes isolates is not sufficient to elucidate the true pathogenic potential, because both the gene are regulated by a key regulator gene i.e., prfA (Shakuntala et al, 2006;Kaur et al, 2007;Rawool et al, 2007b;Aurora et al, 2008). In addition, other genes such as actA, internalins and several other virulent associated genes do play an essential role in pathogenesis of this bug.…”

Section: Discussionmentioning

confidence: 99%

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Isolation and Identification of pathogenic Listeria monocytogenes from diarrhoeal cases in human infants and young animals

Negi¹

2014

Adv. Anim. Vet. Sci.

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Studies addressing isolation of Listeria monocytogenes from febrile gastroenteritis cases are very sparse. In the present study sporadic febrile diarrheal cases in human infants (less than 5 years of age) and young animals (0 -6 months) were initially analyzed for isolation of L. monocytogenes. The isolates recovered were further characterized for their virulence potential using in vitro and in vivo pathogenicity tests and serogrouping by multiplex serotyping PCR. A total of 315 diarrhoeal samples (165 human infants and 150 young animals) were collected and analyzed for isolation of L. monocytogenes. On microbiological and biochemical analysis, four L. monocytogenes isolates were identified, two each from human infants and piglet respectively. The overall isolation rate of L. monocytogenes was very low in human infants (1.2%) and piglets (1.3%); however all the isolates were found to be highly pathogenic when assessed using in vitro and in vivo assays. Further, serotyping revealed that all the four L. monocytogenes isolates, belonged to serogroup 4b, 4d, 4e, which is a matter of great concern as serovar 4b in particular, is generally associated with listerial foodborne outbreaks worldwide. To conclude, isolation of pathogenic L. monocytogenes from sporadic febrile diarrheal cases (human infants and piglets) appears to be the first report in India. Thus, from future human and animal health perspective, due consideration should be imposed for either isolation or detection of L. monocytogenes from febrile gastroenteritis cases. All copyrights reserved to Nexus® academic publishers

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Isolation and Identification of pathogenic Listeria monocytogenes from diarrhoeal cases in human infants and young animals

Negi¹

2014

Adv. Anim. Vet. Sci.

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“…In contrast with this, Aurora et al (2) was found 93%, Gouws and Liedemann (23) 84% correlation between chromogenic agar and the PCR results.…”

Section: Discussionmentioning

confidence: 75%

“…The last isolate was resistant to streptomycin, meropenem, trimethoprim/ sulfamethoxazole and was intermediate to gentamicin. Resistance to streptomycin, meropenem, trimethoprim/ sulfamethoxazole was most common with 4 isolates showing resistance, then gentamicin (3 resistant isolates and 1 intermediate), penicillin G and rifampicin [3], and finally, erythromycin and tetracycline [2]. All isolates were sensitive to ampicillin (Table 4).…”

Section: Resultsmentioning

confidence: 99%

Isolation of Listeria monocytogenes in lamb meat and determination of the antibiotic resistance

SARIMEHMETOĞLU

2017

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:In this study the occurrence of Listeria monocytogenes and other Listeria species were investigated from a total of 120 retail raw lamb meat samples and L. monocytogenes isolates were verified by using PCR based on hylA gene which is responsible for virulence. All L. monocytogenes isolates were analyzed by antimicrobial susceptibility tests. L. monocytogenes was isolated from 19 (15.8%) of the samples examined. The other species isolated were L. innocua, L. weshimeri and L. grayi 26 (21.6%), nine (7.5%) 2 (1.6%) strains respectively. According to the PCR analysis, hlyA gene was detected from the 58 of the isolates which were isolated from L. monocytogenes positive 19 lamb meat samples. The antibiotic resistance profiles of the 58 isolates to nine antibiotics were detected by disc diffusion method. Five (8.6%) isolates were found to be resistant to antibiotics. Among the five isolates, four (6.9%) displayed multiple resistance, only one isolate was resistant to tetracycline.Keywords: Antimicrobial resistance, food safety, lamb meat, Listeria, PCR. Kuzu etlerinden Listeria monocytogenes izolasyonu ve antibiyotik dirençliliklerinin belirlenmesi

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“…Previous workers had reported the isolation of the organism from fresh raw milk (23,24,25) This supports the presence of this organism in food (raw milk) across the six zones in Nigeria..The presence of this organism in fresh raw milk in this study agreed with the reports of (26,27) who reported that the organism could be as high as 5% in some samples and attributed the contamination of fresh raw milk to mastitis caused by the organism or of faecal origin.…”

Section: Discussion/conclusionmentioning

confidence: 99%

Detection of Plasmid in Listeria monocytogenes Isolated from Fresh Raw Milk in Nigeria

Uwanibe¹

2014

AVS

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Abstract:Aims: To determine the presence of plasmids in Listeria monocytogenes strains isolated from fresh raw milk from healthy cattle in Nigeria and also determine its role in antibiotics resistance since this organism is a food borne pathogen of public health importance. Methods and results: Isolation of Listeria monocytogenes was done using selective enrichment and isolation protocol, as recommended by United States Department of Agriculture (USDA) and Association of Analytical Chemists/ International Dairy Federation (AOAC/ IDF). Identification was by phenotypic characterization and confirmed using β-haemolytic activity, phosphatidyl inositol phospholipase-C (PIPLC) assay and polymerase chain reaction (PCR) . Strains identified as Listeria monocytogenes from the six zones were determined and results expressed as rate of prevalence in graph. Twenty-three strains of Listeria monocytogenes isolated from 305 samples of fresh raw milk were screened for the presence of plasmid DNA using the method of Ehrenfeld and Clewel and agarose gel electrophoresis. One isolate was found to harbour one plasmid with molecular weight of 14.62kb. The plasmid was cured using different concentrations of acridine orange. Antibiotic sensitivity test was carried out on both the wild strain (with plasmid) and cured strain using disc diffusion method of Bauer-Kirby and it was found that the wild strain was resistant to seven of the twelve antibiotics used while the cured strain was resistant to only one. Conclusions: There is a possibility of the presence of strains of Listeria monocytogenes that can harbour plasmids. This could confer antibiotic resistance to the strains and thus make antibiotic therapy unsuccessful. The increase in the number of antibiotics which the cured strain of Listeria monocytogenes was susceptible to in this study showed that plasmid may play a significant role in antibiotics resistance. The Fulani herdsmen who take fresh raw milk without boiling or pasteurization may serve as carriers of resistant strains of Listeria monocytogenes and help in its spread in the environment through faecal pollution.

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Comparison of PI-PLC based assays and PCR along with in vivo pathogenicity tests for rapid detection of pathogenic Listeria monocytogenes

Cited by 25 publications

References 33 publications

Isolation and Identification of pathogenic Listeria monocytogenes from diarrhoeal cases in human infants and young animals

Isolation and Identification of pathogenic Listeria monocytogenes from diarrhoeal cases in human infants and young animals

Isolation of Listeria monocytogenes in lamb meat and determination of the antibiotic resistance

Detection of Plasmid in Listeria monocytogenes Isolated from Fresh Raw Milk in Nigeria

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